FastQCFastQC Report
Thu 26 May 2016
SRR1926775_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926775_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834614
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT25230.30229543237951917No Hit
CTGTAGGACGTGGAATATGGCAAGA19640.23531836274014095No Hit
GTCCTAAAGTGTGTATTTCTCATTT17280.20704181813389183No Hit
GTCCTACAGTGTGCATTTCTCATTT16870.20212936758789093No Hit
CTTTAGGACGTGAAATATGGCGAGG13470.1613919728161761No Hit
CTGTAGGACCTGGAATATGGCGAGA9780.11717991790216795No Hit
GTACATGGGAAGCAGTGGTATCAAC8660.1037605408008972No Hit
GTATCAACGCAGAGTACTTTTTTTT8560.10256238213114087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTT704.188896E-715.0021421
CGCCTTG902.1645974E-914.8327072
TAGGACC4050.014.8130694
GTAGGAC11350.013.1898153
TGTAGGA11950.012.9265322
CTGTAGG11700.012.8922831
TGGCGAT604.15169E-412.64365518
GACGTGG5650.012.6300957
GTCCTAT1650.012.1504951
AGGACGT10600.012.1177435
GGATAGT550.003031148312.1095816
AGGACCT7100.012.0608515
GGACGTG10550.011.99486
ACTGTTC2700.011.9713618
TCCAACG1201.0255462E-811.85342618
TAGGACG11100.011.8390164
ACGTGGA5900.011.7624878
GGACCTG7300.011.7304166
GTCTAGG907.0519873E-611.6683321
GTCCTAG1655.456968E-1211.57191
Produced by FastQC (version 0.11.5)