##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926775_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 834614 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.38367556738804 30.0 26.0 31.0 10.0 31.0 2 27.032764846983156 31.0 28.0 31.0 10.0 34.0 3 26.655857677920572 31.0 27.0 31.0 10.0 34.0 4 30.343570800393955 35.0 31.0 37.0 10.0 37.0 5 29.9260472505853 35.0 30.0 37.0 10.0 37.0 6 29.725803784743604 35.0 30.0 37.0 8.0 37.0 7 29.762580066953106 35.0 30.0 37.0 2.0 37.0 8 29.476923464020494 35.0 29.0 37.0 2.0 37.0 9 31.663174832916773 37.0 32.0 39.0 2.0 39.0 10 31.843328772342662 37.0 32.0 39.0 2.0 39.0 11 32.04189002341202 37.0 32.0 39.0 2.0 39.0 12 31.894397889323688 37.0 32.0 39.0 2.0 39.0 13 32.0121109878339 37.0 32.0 39.0 2.0 39.0 14 32.95624444353917 38.0 32.0 40.0 2.0 41.0 15 32.534750195898944 38.0 32.0 40.0 2.0 41.0 16 32.59527278478434 38.0 32.0 40.0 2.0 41.0 17 32.81438964599204 38.0 32.0 40.0 2.0 41.0 18 32.865384477135535 38.0 32.0 40.0 2.0 41.0 19 32.24059864799776 38.0 31.0 40.0 2.0 41.0 20 32.51168444334746 39.0 32.0 40.0 2.0 41.0 21 32.48728514019655 39.0 32.0 40.0 2.0 41.0 22 32.51453366466414 39.0 32.0 40.0 2.0 41.0 23 32.49927870848081 39.0 32.0 40.0 2.0 41.0 24 31.22186064456144 38.0 30.0 40.0 2.0 41.0 25 31.760325132336625 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.3483449877108988 1101 2 -1.3198830399590094 1101 3 -2.181889871141184 1101 4 -1.504675096649084 1101 5 -1.8516534740206119 1101 6 -3.482261398792904 1101 7 -1.991617436378565 1101 8 -1.5144185285318308 1101 9 -1.6043321198153428 1101 10 -9.385738302374925 1101 11 -4.969671873174818 1101 12 -2.7527161348434674 1101 13 -2.0569782917048087 1101 14 -1.810348990745755 1101 15 -3.2342696352074256 1101 16 -1.7595930361762733 1101 17 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1.692035348075688 2215 8 0.660870842034381 2215 9 1.1130499250797072 2215 10 1.026276439281908 2215 11 0.9416398720800316 2215 12 0.6724652953303156 2215 13 0.9544421222852009 2215 14 1.1476128149615406 2215 15 1.1482746116133598 2215 16 1.3992544342894249 2215 17 0.9869228383491233 2215 18 0.32008024164097293 2215 19 1.4864253578530864 2215 20 -2.214207206089803 2215 21 -0.7046515586182736 2215 22 0.3029759068037521 2215 23 0.46178813750773884 2215 24 0.7520930257751672 2215 25 1.0167466031994188 2216 1 0.5171358466892819 2216 2 -0.5029932139178896 2216 3 0.44900100919619135 2216 4 0.20859548095465286 2216 5 0.5490574228842746 2216 6 -0.8092468645659281 2216 7 -8.80756140806318 2216 8 -9.896582848134212 2216 9 -5.025571670986363 2216 10 -2.777334055067147 2216 11 -1.7381002186827637 2216 12 -1.4827048388121398 2216 13 -1.248860210523997 2216 14 -1.2055956207630771 2216 15 -2.0898852689147844 2216 16 -1.228098194697246 2216 17 -1.6187826276991117 2216 18 -1.241665058895542 2216 19 -0.7208330316472988 2216 20 -1.5822753492980013 2216 21 -4.042147443213093 2216 22 -2.6395385333299473 2216 23 -2.6172624088863756 2216 24 -2.628258383734643 2216 25 -2.1418254236343373 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 73791.0 3 17.0 4 574.0 5 2751.0 6 3219.0 7 2667.0 8 2138.0 9 2024.0 10 1888.0 11 1837.0 12 1715.0 13 1786.0 14 1757.0 15 1879.0 16 1941.0 17 2177.0 18 2535.0 19 3079.0 20 3996.0 21 5158.0 22 6291.0 23 7647.0 24 8498.0 25 9713.0 26 10923.0 27 11767.0 28 13398.0 29 15730.0 30 18559.0 31 22398.0 32 28258.0 33 37161.0 34 50022.0 35 68343.0 36 97443.0 37 149800.0 38 161734.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.75125438042374 18.70401906129831 12.449290365349558 26.095436192928396 2 13.763550521623843 20.083387825377493 42.734040830038815 23.419020822959848 3 19.771882735343418 24.15616889700527 29.115067714063912 26.9568806535874 4 11.388027936812446 16.7315128443392 38.875693174665024 33.00476604418333 5 12.077311476274312 37.93904776058227 36.51885027523068 13.464790487912742 6 30.785575864056746 37.72942940315697 19.306885009353948 12.17810972343233 7 26.983396441356405 30.8978563300091 23.726613881041274 18.392133347593223 8 24.518684255429598 38.55116210324947 21.060733142833797 15.869420498487138 9 25.869751232572018 15.590328294388772 20.53451800861422 38.00540246442499 10 13.69729194559679 30.20068585346325 35.68032180695179 20.421700393988175 11 34.052122393071436 22.800041703666686 24.977021039979196 18.17081486328268 12 22.83506841041214 25.10375109802996 32.76916863702874 19.292011854529157 13 28.90971122884428 22.676630711579968 25.7306759883854 22.682982071190352 14 19.50666788104232 23.376651391099266 28.133328859317775 28.98335186854064 15 22.37192469471641 32.87511833858617 23.092861337136146 21.66009562956128 16 20.658888948809672 29.00669315165351 29.686674216685542 20.64774368285127 17 20.601650452160616 29.701068263909363 28.87680091271011 20.82048037121991 18 20.394430942828244 28.606489404027474 31.083037336362807 19.91604231678147 19 23.337416136741158 27.384904493560597 27.37723425623866 21.90044511345959 20 22.59757348570552 28.655710122140626 28.86794623511591 19.878770157037945 21 21.68656362272399 27.807004719185684 27.405190864576866 23.101240793513455 22 21.80078543035088 29.75275841365599 27.901352632342135 20.545103523650994 23 21.004297523830527 29.284521004657048 28.37276525165561 21.33841621985681 24 22.966534126179504 27.891383909632793 28.916835038623688 20.225246925564015 25 21.617469417494345 28.457788472600377 28.916526057445257 21.00821605246002 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 329.0 1 329.0 2 441.0 3 553.0 4 553.0 5 553.0 6 715.5 7 878.0 8 878.0 9 878.0 10 1309.5 11 1741.0 12 1741.0 13 1741.0 14 2969.5 15 4198.0 16 4198.0 17 4198.0 18 7261.5 19 10325.0 20 10325.0 21 10325.0 22 16394.0 23 22463.0 24 22463.0 25 22463.0 26 32829.0 27 43195.0 28 43195.0 29 43195.0 30 57411.0 31 71627.0 32 71627.0 33 71627.0 34 80720.5 35 89814.0 36 89814.0 37 89814.0 38 96018.5 39 102223.0 40 102223.0 41 102223.0 42 105524.0 43 108825.0 44 108825.0 45 108825.0 46 110241.5 47 111658.0 48 111658.0 49 111658.0 50 104030.0 51 96402.0 52 96402.0 53 96402.0 54 83090.0 55 69778.0 56 69778.0 57 69778.0 58 58304.5 59 46831.0 60 46831.0 61 46831.0 62 37287.0 63 27743.0 64 27743.0 65 27743.0 66 21246.0 67 14749.0 68 14749.0 69 14749.0 70 10795.0 71 6841.0 72 6841.0 73 6841.0 74 4835.0 75 2829.0 76 2829.0 77 2829.0 78 1919.0 79 1009.0 80 1009.0 81 1009.0 82 704.5 83 400.0 84 400.0 85 400.0 86 267.5 87 135.0 88 135.0 89 135.0 90 92.0 91 49.0 92 49.0 93 49.0 94 31.5 95 14.0 96 14.0 97 14.0 98 9.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23148425499692074 2 0.13886658982475733 3 0.14174217063217248 4 0.07057154564864715 5 0.026718938335565906 6 0.02480188446395579 7 0.29271016302146863 8 0.2894751346131266 9 0.021566856055613735 10 0.038940156767080354 11 0.018571459381222937 12 0.019769618050979255 13 0.01761293244541788 14 0.019889433917954887 15 0.017253484844490987 16 0.0214470401886381 17 0.021087592587711205 18 0.017493116578442252 19 0.026359490734639005 20 0.019889433917954887 21 0.017732748312393513 22 0.020009249784930516 23 0.021566856055613735 24 0.018212011780296043 25 0.017972380046344775 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 834614.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.4583242411085 #Duplication Level Percentage of deduplicated Percentage of total 1 94.09726124025194 80.41394261269743 2 4.484826672019944 7.665315438053037 3 0.7196641877119672 1.845038864946098 4 0.2366189447951826 0.8088423402358267 5 0.11080125504102777 0.4734444789808954 6 0.06822539434911266 0.3498256723058377 7 0.04744124257132605 0.2837974363043023 8 0.03397663759834367 0.2322869210001513 9 0.02626596544863174 0.20201808526334317 >10 0.14253169137553595 2.302581997277104 >50 0.017106227408583793 1.0170421512197214 >100 0.013317903214383371 2.526279521453252 >500 0.0012616959946987279 0.771529355146496 >1k 7.009422192770711E-4 1.1080551251164508 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2523 0.30229543237951917 No Hit CTGTAGGACGTGGAATATGGCAAGA 1964 0.23531836274014095 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1728 0.20704181813389183 No Hit GTCCTACAGTGTGCATTTCTCATTT 1687 0.20212936758789093 No Hit CTTTAGGACGTGAAATATGGCGAGG 1347 0.1613919728161761 No Hit CTGTAGGACCTGGAATATGGCGAGA 978 0.11717991790216795 No Hit GTACATGGGAAGCAGTGGTATCAAC 866 0.1037605408008972 No Hit GTATCAACGCAGAGTACTTTTTTTT 856 0.10256238213114087 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 2.396317339512637E-4 0.0 12 0.0 0.0 0.0 2.396317339512637E-4 0.0 13 0.0 0.0 0.0 2.396317339512637E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCCTT 70 4.188896E-7 15.002142 1 CGCCTTG 90 2.1645974E-9 14.832707 2 TAGGACC 405 0.0 14.813069 4 GTAGGAC 1135 0.0 13.189815 3 TGTAGGA 1195 0.0 12.926532 2 CTGTAGG 1170 0.0 12.892283 1 TGGCGAT 60 4.15169E-4 12.643655 18 GACGTGG 565 0.0 12.630095 7 GTCCTAT 165 0.0 12.150495 1 AGGACGT 1060 0.0 12.117743 5 GGATAGT 55 0.0030311483 12.109581 6 AGGACCT 710 0.0 12.060851 5 GGACGTG 1055 0.0 11.9948 6 ACTGTTC 270 0.0 11.971361 8 TCCAACG 120 1.0255462E-8 11.853426 18 TAGGACG 1110 0.0 11.839016 4 ACGTGGA 590 0.0 11.762487 8 GGACCTG 730 0.0 11.730416 6 GTCTAGG 90 7.0519873E-6 11.668332 1 GTCCTAG 165 5.456968E-12 11.5719 1 >>END_MODULE