FastQCFastQC Report
Thu 26 May 2016
SRR1926775_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926775_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834614
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT25040.3000189309069822No Hit
CTGTAGGACGTGGAATATGGCAAGA23740.28444286820014997No Hit
GTCCTAAAGTGTGTATTTCTCATTT17820.21351187495057597No Hit
GTCCTACAGTGTGCATTTCTCATTT16850.20188973585393966No Hit
CTTTAGGACGTGAAATATGGCGAGG16710.20021231371628082No Hit
GTATCAACGCAGAGTACTTTTTTTT15590.18679293661501004No Hit
CTGTAGGACCTGGAATATGGCGAGA12940.1550417318664676No Hit
GTACATGGGAAGCAGTGGTATCAAC10390.12448868578768149No Hit
GGTATCAACGCAGAGTACTTTTTTT9490.11370525775987464No Hit
CTGAAGGACCTGGAATATGGCGAGA9420.11286654669104519No Hit
CCCATGTACTCTGCGTTGATACCAC8790.10531814707158041No Hit
TATCAACGCAGAGTACTTTTTTTTT8660.1037605408008972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGA5600.012.70688157
GTCCTAT1301.8553692E-1012.4706551
AGGACGA701.0634082E-412.2463115
GGACGTG11250.012.191156
GGACGAG550.003008186112.1218816
GTCCTAC13700.012.1119021
AGGACGT11500.012.0924935
CTAAATT7400.012.05279819
ACTGTTC3550.012.0246328
GATATAC3750.011.6979641
TTAGGAC7350.011.6631543
ACGTGAA6400.011.5611838
TCCTACA14450.011.5471672
GGCGAGG4850.011.54255719
CGTGAAA6500.011.5299499
CTTTAGG7700.011.5179691
TAGGACC5800.011.4955794
GGACCTT752.022958E-411.4292026
ATGCCGG1001.9544404E-611.38604719
GCTGACC1001.9600866E-611.3833198
Produced by FastQC (version 0.11.5)