##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926775_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 834614 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.718009762596843 31.0 30.0 33.0 25.0 34.0 2 30.038297943720092 31.0 30.0 34.0 26.0 34.0 3 30.12192702255174 31.0 30.0 34.0 26.0 34.0 4 33.7428116470608 35.0 35.0 37.0 28.0 37.0 5 33.7257486694448 37.0 35.0 37.0 28.0 37.0 6 33.67322259152135 37.0 35.0 37.0 29.0 37.0 7 33.63880069109792 37.0 35.0 37.0 28.0 37.0 8 33.66949152542373 37.0 35.0 37.0 29.0 37.0 9 35.17009300107595 39.0 35.0 39.0 29.0 39.0 10 35.060897612549034 39.0 35.0 39.0 28.0 39.0 11 35.11117474664935 39.0 35.0 39.0 29.0 39.0 12 35.004680007764065 39.0 35.0 39.0 28.0 39.0 13 35.02230971443086 39.0 35.0 39.0 28.0 39.0 14 36.08824678234489 40.0 36.0 41.0 28.0 41.0 15 36.09013148593242 40.0 36.0 41.0 28.0 41.0 16 36.06948122125917 40.0 36.0 41.0 27.0 41.0 17 36.05256561715955 40.0 36.0 41.0 27.0 41.0 18 36.02601442103775 40.0 36.0 41.0 27.0 41.0 19 36.08948927288543 40.0 37.0 41.0 27.0 41.0 20 36.06628093945225 40.0 37.0 41.0 27.0 41.0 21 36.001201753145764 40.0 37.0 41.0 27.0 41.0 22 35.913918290371356 40.0 36.0 41.0 27.0 41.0 23 35.85174703515637 40.0 36.0 41.0 27.0 41.0 24 35.7936794733853 40.0 36.0 41.0 27.0 41.0 25 35.67247254419408 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.011069223740562961 1101 2 0.006504167455279486 1101 3 0.3677148476012526 1101 4 0.28475913298966304 1101 5 0.18158764255981197 1101 6 0.05828289400389508 1101 7 0.015214422527549232 1101 8 -0.06510064146457495 1101 9 0.04651834284823053 1101 10 0.18199549695295048 1101 11 0.18941270272273414 1101 12 0.26483505557080633 1101 13 0.3259478789082877 1101 14 0.33116209412099806 1101 15 0.35625558883280206 1101 16 0.46620787180034995 1101 17 0.267094331865394 1101 18 0.31128456734244736 1101 19 0.28365868100701164 1101 20 0.24676965825565844 1101 21 0.28389196117166904 1101 22 0.11009366286812394 1101 23 0.2619118425474909 1101 24 0.1969540585213707 1101 25 0.141688864973041 1102 1 -0.1293685995502436 1102 2 -0.05570624020324644 1102 3 0.13517282832033573 1102 4 0.15742094297334575 1102 5 -0.004855818583919813 1102 6 0.08039033549656693 1102 7 0.2601130546927024 1102 8 0.09684091009422247 1102 9 0.20791343344836122 1102 10 0.20858432209553968 1102 11 0.33588712101436613 1102 12 0.27100764167037283 1102 13 0.3509760600076035 1102 14 0.34624024409491483 1102 15 0.2059196028308179 1102 16 0.4503456984066787 1102 17 0.4336209062214351 1102 18 0.36519913234404555 1102 19 0.4227854875416668 1102 20 0.27551606201089385 1102 21 0.43851349321399624 1102 22 0.4806702397478446 1102 23 0.4439822700589744 1102 24 0.38013288652413024 1102 25 0.5456715693491034 1103 1 -0.377130530854501 1103 2 -0.41005371075673125 1103 3 -0.22575784511955987 1103 4 -0.4589242512952225 1103 5 -0.44840161630162356 1103 6 -0.3339008620896422 1103 7 -0.5157316239072145 1103 8 -0.5235588749604005 1103 9 -0.5375385025620005 1103 10 -0.3538022391455371 1103 11 -0.36448302560908274 1103 12 -0.49055451726237465 1103 13 -0.4713255243801555 1103 14 -0.46511981945415215 1103 15 -0.4175922174962068 1103 16 -0.411227474807923 1103 17 -0.4635377428268299 1103 18 -0.4511974619065029 1103 19 -0.40214483878146723 1103 20 -0.34531494886654457 1103 21 -0.31041027794260145 1103 22 -0.3177804179382875 1103 23 -0.29723217133336277 1103 24 -0.368360326341886 1103 25 -0.1735817290945505 1104 1 -0.36948292360536783 1104 2 -0.2994348011921133 1104 3 -0.18688825546962917 1104 4 -0.3192870911782393 1104 5 -0.5265022306134313 1104 6 -0.5770418983736292 1104 7 -0.39636994623572974 1104 8 -0.4039353804074679 1104 9 -0.486552538501158 1104 10 -0.4131574033238792 1104 11 -0.5244384528814621 1104 12 -0.5044838139328292 1104 13 -0.5554562284759896 1104 14 -0.5615469768891117 1104 15 -0.462528934440229 1104 16 -0.45036973716121054 1104 17 -0.5683328232237699 1104 18 -0.6569810606753208 1104 19 -0.5657574008831574 1104 20 -0.734985315027032 1104 21 -0.6386175347402627 1104 22 -0.6429231400675661 1104 23 -0.6297555752082502 1104 24 -0.6371958159394566 1104 25 -0.5767216159385669 1105 1 -0.11823659614974602 1105 2 -0.08675572620577299 1105 3 -0.041139302483117035 1105 4 0.08385493355638118 1105 5 -0.001336996874741203 1105 6 -0.12414889306351284 1105 7 -0.2993680219947237 1105 8 -0.3547392800713425 1105 9 -0.2703054974523198 1105 10 0.033439987385627035 1105 11 -0.07614812379195968 1105 12 -0.0226570645221571 1105 13 0.05394622333185595 1105 14 -0.08001304676218979 1105 15 -0.14358967844727744 1105 16 -0.008913908581064334 1105 17 -0.14240995034671045 1105 18 -0.24089347079949164 1105 19 -0.15363763533543562 1105 20 -0.07957947734242055 1105 21 -0.13723956164026418 1105 22 -0.08368136976583429 1105 23 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0.4977762078330912 2216 1 -0.035507810983578025 2216 2 0.024336835577880578 2216 3 -1.9874173159652813 2216 4 -1.2140133649393974 2216 5 -0.5796504024736961 2216 6 -0.26165083594565175 2216 7 -0.37839352987850106 2216 8 -0.18653749664944996 2216 9 -0.24132863358072143 2216 10 -0.17758579681917297 2216 11 -0.45619314109080733 2216 12 -0.3608325398806542 2216 13 -0.40632111725106057 2216 14 -0.622569361560906 2216 15 -0.5109964632795538 2216 16 -0.27344119815973755 2216 17 -0.3596938298937715 2216 18 -0.4611501039430408 2216 19 -0.4422273043834508 2216 20 -0.47863135085647457 2216 21 -0.30542349932987634 2216 22 -0.4740527336978815 2216 23 -0.5270602186016902 2216 24 -0.8518447968141203 2216 25 -0.5562221223000989 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22118.0 3 19.0 4 315.0 5 2515.0 6 3408.0 7 2451.0 8 1882.0 9 1528.0 10 1348.0 11 1256.0 12 1183.0 13 1100.0 14 1109.0 15 1064.0 16 1134.0 17 1140.0 18 1323.0 19 1545.0 20 2016.0 21 2517.0 22 3295.0 23 4037.0 24 4712.0 25 5463.0 26 6614.0 27 7208.0 28 8370.0 29 10334.0 30 13162.0 31 16895.0 32 21905.0 33 28680.0 34 39200.0 35 54772.0 36 80321.0 37 139983.0 38 338692.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.567147438012164 19.611776038848788 10.214241756448907 28.606834766690138 2 13.581068842209563 21.21382242263064 41.0760876215016 24.1290211136582 3 18.747056617203857 24.718423868692476 29.230592247564108 27.303927266539564 4 11.504000651798316 17.494913816446882 38.78032240053486 32.22076313121994 5 12.27392657811051 39.16573006108506 36.059834118963686 12.50050924184075 6 31.420487748569727 38.78276887855344 17.78747994969086 12.009263423185972 7 27.853557869305106 31.78245800736309 21.878115892007976 18.485868231323824 8 24.112015146249984 39.797251147352405 19.862916851401387 16.227816854996224 9 25.920396102246528 15.70329238423452 19.98593223507307 38.390379278445884 10 13.819732512802815 30.60993407478235 34.987215156809036 20.5831182556058 11 34.12542551190235 22.922345650462567 24.839231089058845 18.112997748576234 12 23.020692299397325 25.284144690334408 32.49134326212243 19.20381974814584 13 29.096329170495817 22.71476275660281 25.706949498386667 22.481958574514714 14 19.6602261644305 23.65632495980178 27.733898544716478 28.94955033105124 15 22.032176162512744 33.25429281098691 23.021161212710865 21.692369813789476 16 20.804137990529686 29.30405651515442 29.251816421601518 20.639989072714375 17 20.523428850771378 30.00723689570723 28.645885752900753 20.823448500620646 18 20.273685042936272 28.70709517870045 31.08353732106891 19.935682457294373 19 22.739134498103315 27.673990611228668 27.72047916761521 21.86639572305281 20 22.57829133951668 28.8056534392834 28.75113676257208 19.864918458627834 21 21.5341180756358 27.75857131045094 27.622921058024037 23.084389555889224 22 21.85489339982315 29.878003484245415 27.73473725578531 20.532365860146125 23 20.983352783442406 29.36543120532366 28.259291121404623 21.391924889829312 24 22.28266274309171 27.85482612899631 29.33263680292543 20.52987432498655 25 21.533827162860696 28.73733173889183 28.824323061327682 20.90451803691979 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 317.0 1 317.0 2 304.0 3 291.0 4 291.0 5 291.0 6 495.0 7 699.0 8 699.0 9 699.0 10 1140.0 11 1581.0 12 1581.0 13 1581.0 14 2811.5 15 4042.0 16 4042.0 17 4042.0 18 6989.0 19 9936.0 20 9936.0 21 9936.0 22 16044.5 23 22153.0 24 22153.0 25 22153.0 26 32561.0 27 42969.0 28 42969.0 29 42969.0 30 56989.0 31 71009.0 32 71009.0 33 71009.0 34 80496.5 35 89984.0 36 89984.0 37 89984.0 38 95793.0 39 101602.0 40 101602.0 41 101602.0 42 105452.5 43 109303.0 44 109303.0 45 109303.0 46 111057.5 47 112812.0 48 112812.0 49 112812.0 50 105770.0 51 98728.0 52 98728.0 53 98728.0 54 84562.5 55 70397.0 56 70397.0 57 70397.0 58 58738.0 59 47079.0 60 47079.0 61 47079.0 62 37425.0 63 27771.0 64 27771.0 65 27771.0 66 20944.0 67 14117.0 68 14117.0 69 14117.0 70 10214.5 71 6312.0 72 6312.0 73 6312.0 74 4348.5 75 2385.0 76 2385.0 77 2385.0 78 1578.5 79 772.0 80 772.0 81 772.0 82 515.0 83 258.0 84 258.0 85 258.0 86 166.5 87 75.0 88 75.0 89 75.0 90 46.0 91 17.0 92 17.0 93 17.0 94 11.0 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12257163191607139 2 0.11777899723704612 3 0.013778824702197661 4 0.0 5 0.004792634679025274 6 0.35417570277996774 7 0.021806487789564996 8 0.010064532825953076 9 0.009824901092001811 10 0.0038341077432202187 11 0.0037142918762445875 12 4.7926346790252733E-4 13 8.38711068829423E-4 14 0.0 15 0.002516133206488269 16 7.188952018537912E-4 17 2.3963173395126367E-4 18 5.990793348781592E-4 19 0.0 20 8.38711068829423E-4 21 0.013778824702197661 22 0.0 23 0.0 24 1.1981586697563183E-4 25 0.005751161614830329 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 834614.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.78824606279468 #Duplication Level Percentage of deduplicated Percentage of total 1 91.89280093055636 75.15751013907752 2 6.247716862162508 10.219796081064372 3 0.9644528276812482 2.3664271555905616 4 0.31170605568049176 1.0197556632503695 5 0.14540914456540593 0.5946379447747951 6 0.08970750039616435 0.4402211469647844 7 0.06219945495848442 0.3561029028981366 8 0.0448054853361108 0.2931649647706215 9 0.031228416137462772 0.22987056448819376 >10 0.1713605609262888 2.627235698348529 >50 0.019675076432616182 1.1223114148893507 >100 0.015267591297572233 2.5430448884593546 >500 0.0024956638311750207 1.360012684725226 >1k 0.0011744300382000097 1.669908750698194 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2504 0.3000189309069822 No Hit CTGTAGGACGTGGAATATGGCAAGA 2374 0.28444286820014997 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1782 0.21351187495057597 No Hit GTCCTACAGTGTGCATTTCTCATTT 1685 0.20188973585393966 No Hit CTTTAGGACGTGAAATATGGCGAGG 1671 0.20021231371628082 No Hit GTATCAACGCAGAGTACTTTTTTTT 1559 0.18679293661501004 No Hit CTGTAGGACCTGGAATATGGCGAGA 1294 0.1550417318664676 No Hit GTACATGGGAAGCAGTGGTATCAAC 1039 0.12448868578768149 No Hit GGTATCAACGCAGAGTACTTTTTTT 949 0.11370525775987464 No Hit CTGAAGGACCTGGAATATGGCGAGA 942 0.11286654669104519 No Hit CCCATGTACTCTGCGTTGATACCAC 879 0.10531814707158041 No Hit TATCAACGCAGAGTACTTTTTTTTT 866 0.1037605408008972 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.1981586697563185E-4 0.0 7 0.0 0.0 0.0 1.1981586697563185E-4 0.0 8 1.1981586697563185E-4 0.0 0.0 1.1981586697563185E-4 0.0 9 1.1981586697563185E-4 0.0 0.0 2.396317339512637E-4 0.0 10 1.1981586697563185E-4 0.0 0.0 2.396317339512637E-4 0.0 11 1.1981586697563185E-4 0.0 0.0 2.396317339512637E-4 0.0 12 1.1981586697563185E-4 0.0 0.0 2.396317339512637E-4 0.0 13 1.1981586697563185E-4 0.0 0.0 2.396317339512637E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGTGA 560 0.0 12.7068815 7 GTCCTAT 130 1.8553692E-10 12.470655 1 AGGACGA 70 1.0634082E-4 12.246311 5 GGACGTG 1125 0.0 12.19115 6 GGACGAG 55 0.0030081861 12.121881 6 GTCCTAC 1370 0.0 12.111902 1 AGGACGT 1150 0.0 12.092493 5 CTAAATT 740 0.0 12.052798 19 ACTGTTC 355 0.0 12.024632 8 GATATAC 375 0.0 11.697964 1 TTAGGAC 735 0.0 11.663154 3 ACGTGAA 640 0.0 11.561183 8 TCCTACA 1445 0.0 11.547167 2 GGCGAGG 485 0.0 11.542557 19 CGTGAAA 650 0.0 11.529949 9 CTTTAGG 770 0.0 11.517969 1 TAGGACC 580 0.0 11.495579 4 GGACCTT 75 2.022958E-4 11.429202 6 ATGCCGG 100 1.9544404E-6 11.386047 19 GCTGACC 100 1.9600866E-6 11.383319 8 >>END_MODULE