FastQCFastQC Report
Thu 26 May 2016
SRR1926774_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926774_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13636
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA7825.7348195951892045No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA2711.9873863303021415No Hit
GTACATGGGAAGCAGTGGTATCAAC2431.7820475212672338No Hit
GAGTAAGCAGTGGTATCAACGCAGA2361.7307128190085068No Hit
CCCATGTACTCTGCGTTGATACCAC2021.4813728366089762No Hit
GCTTACTCTGCGTTGATACCACTGC1581.158697565268407No Hit
GAGTACATGGGAAGCAGTGGTATCA940.6893517160457613No Hit
CTGCTTACTCTGCGTTGATACCACT920.6746846582575535No Hit
CATGTACTCTGCGTTGATACCACTG910.6673511293634496No Hit
GCGTTGATACCACTGCTTACTCTGC850.6233499559988267No Hit
GGTATCAACGCAGAGTACATGGGAA720.5280140803754767No Hit
GTATCAACGCAGAGTACTTTTTTTT720.5280140803754767No Hit
GAACAAAAAAAAAAAAAAAAAAAAA720.5280140803754767No Hit
GCGTTGATACCACTGCTTCCCATGT700.5133470225872689No Hit
TATCAACGCAGAGTACATGGGAAGC660.48401290701085364No Hit
AAACAAAAAAAAAAAAAAAAAAAAA610.4473452625403344No Hit
GTATCAACGCAGAGTACATGGGAAG600.44001173364623053No Hit
ACTCTGCGTTGATACCACTGCTTCC580.42534467585802294No Hit
GTGGTATCAACGCAGAGTACATGGG580.42534467585802294No Hit
ACGCAGAGTACATGGGAAGCAGTGG570.41801114696391906No Hit
GGTATCAACGCAGAGTACTTTTTTT480.3520093869169844No Hit
GTGGTATCAACGCAGAGTAAGCAGT460.33734232912877676No Hit
ACGCAGAGTAAGCAGTGGTATCAAC460.33734232912877676No Hit
ACACAAAAAAAAAAAAAAAAAAAAA410.30067468465825753No Hit
GATACCACTGCTTCCCATGTACTCT410.30067468465825753No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA410.30067468465825753No Hit
GGTATCAACGCAGAGTAAGCAGTGG390.2860076268700498No Hit
GTATCAACGCAGAGTAAGCAGTGGT380.27867409797594606No Hit
TATCAACGCAGAGTAAGCAGTGGTA360.26400704018773835No Hit
CAGTGGTATCAACGCAGAGTACATG350.25667351129363447No Hit
AAGCAGTGGTATCAACGCAGAGTAA350.25667351129363447No Hit
GTACTCTGCGTTGATACCACTGCTT340.24933998239953065No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA330.24200645350542682No Hit
ATACCACTGCTTCCCATGTACTCTG330.24200645350542682No Hit
GCTTCCCATGTACTCTGCGTTGATA330.24200645350542682No Hit
ACTCTGCGTTGATACCACTGCTTAC310.22733939571721912No Hit
GTAAGCAGTGGTATCAACGCAGAGT300.22000586682311526No Hit
CATGGGAAGCAGTGGTATCAACGCA300.22000586682311526No Hit
GCAGTGGTATCAACGCAGAGTACAT290.21267233792901147No Hit
GATACCACTGCTTACTCTGCGTTGA290.21267233792901147No Hit
CCACTGCTTACTCTGCGTTGATACC290.21267233792901147No Hit
TATCAACGCAGAGTACTTTTTTTTT290.21267233792901147No Hit
CTGCTTCCCATGTACTCTGCGTTGA280.20533880903490762No Hit
CCACTGCTTCCCATGTACTCTGCGT270.19800528014080376No Hit
ACATGGGAAGCAGTGGTATCAACGC270.19800528014080376No Hit
TGCTTACTCTGCGTTGATACCACTG270.19800528014080376No Hit
GGGAAGCAGTGGTATCAACGCAGAG270.19800528014080376No Hit
GTTGATACCACTGCTTCCCATGTAC260.1906717512466999No Hit
GCAGAGTACATGGGAAGCAGTGGTA250.18333822235259606No Hit
CCATGTACTCTGCGTTGATACCACT240.1760046934584922No Hit
TACCACTGCTTACTCTGCGTTGATA230.16867116456438838No Hit
GAAAACAAAAAAAAAAAAAAAAAAA230.16867116456438838No Hit
AAGCAGTGGTATCAACGCAGAGTAC230.16867116456438838No Hit
GCAGTGGTATCAACGCAGAGTAAGC220.16133763567028453No Hit
ATGGGAAGCAGTGGTATCAACGCAG220.16133763567028453No Hit
GTTGATACCACTGCTTACTCTGCGT220.16133763567028453No Hit
ATACCACTGCTTACTCTGCGTTGAT210.1540041067761807No Hit
CTTCCCATGTACTCTGCGTTGATAC210.1540041067761807No Hit
ACGCAGAGTACTTTTTTTTTTTTTT210.1540041067761807No Hit
CAGTGGTATCAACGCAGAGTAAGCA200.14667057788207685No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA170.12466999119976532No Hit
ACACAAAAAACAAAAAAAAAAAAAA170.12466999119976532No Hit
AAAAAAAACAAAAAAAAAAAAAAAA160.11733646230566148No Hit
ACTGCTTACTCTGCGTTGATACCAC150.11000293341155763No Hit
TTCCCATGTACTCTGCGTTGATACC150.11000293341155763No Hit
GAAAAAAACAAAAAAAAAAAAAAAA140.10266940451745381No Hit
AAAAACAAAAAAAAAAAAAAAAAAA140.10266940451745381No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)