FastQCFastQC Report
Thu 26 May 2016
SRR1926774_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926774_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13636
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT4693.4394250513347027No Hit
GGTATCAACGCAGAGTACTTTTTTT4072.984746259900264No Hit
TATCAACGCAGAGTACTTTTTTTTT3052.236726312701672No Hit
GTACATGGGAAGCAGTGGTATCAAC2782.038721032560868No Hit
CCCATGTACTCTGCGTTGATACCAC2752.0167204458785566No Hit
GAGTAAGCAGTGGTATCAACGCAGA2151.5767087122323262No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1921.4080375476679377No Hit
GCTTACTCTGCGTTGATACCACTGC1681.2320328542094456No Hit
GAGTACATGGGAAGCAGTGGTATCA1501.1000293341155765No Hit
CATGTACTCTGCGTTGATACCACTG1160.8506893517160459No Hit
CTGCTTACTCTGCGTTGATACCACT990.7260193605162805No Hit
GCGTTGATACCACTGCTTACTCTGC900.6600176004693459No Hit
GAGTACTTTTTTTTTTTTTTTTTTT890.652684071575242No Hit
ACGCAGAGTACATGGGAAGCAGTGG890.652684071575242No Hit
GCGTTGATACCACTGCTTCCCATGT880.6453505426811381No Hit
GTACTTTTTTTTTTTTTTTTTTTTT750.5500146670577882No Hit
GGTATCAACGCAGAGTACATGGGAA740.5426811381636844No Hit
GCAGAGTACTTTTTTTTTTTTTTTT720.5280140803754767No Hit
ACGCAGAGTAAGCAGTGGTATCAAC660.48401290701085364No Hit
TATCAACGCAGAGTACATGGGAAGC640.46934584922264594No Hit
ACTCTGCGTTGATACCACTGCTTCC620.45467879143443823No Hit
ATCAACGCAGAGTACTTTTTTTTTT620.45467879143443823No Hit
GTATCAACGCAGAGTACATGGGAAG600.44001173364623053No Hit
ACTCTGCGTTGATACCACTGCTTAC600.44001173364623053No Hit
GTGGTATCAACGCAGAGTACTTTTT580.42534467585802294No Hit
GGTATCAACGCAGAGTAAGCAGTGG510.374009973599296No Hit
GTGGTATCAACGCAGAGTAAGCAGT490.3593429158110883No Hit
GCTTCCCATGTACTCTGCGTTGATA490.3593429158110883No Hit
GTGGTATCAACGCAGAGTACATGGG460.33734232912877676No Hit
TATCAACGCAGAGTAAGCAGTGGTA430.31534174244646523No Hit
CCACTGCTTACTCTGCGTTGATACC420.3080082135523614No Hit
AAGCAGTGGTATCAACGCAGAGTAA410.30067468465825753No Hit
GTACTCTGCGTTGATACCACTGCTT400.2933411557641537No Hit
AACGCAGAGTACTTTTTTTTTTTTT400.2933411557641537No Hit
GTATCAACGCAGAGTAAGCAGTGGT390.2860076268700498No Hit
GTAAGCAGTGGTATCAACGCAGAGT380.27867409797594606No Hit
ATACCACTGCTTCCCATGTACTCTG380.27867409797594606No Hit
CTGCTTCCCATGTACTCTGCGTTGA380.27867409797594606No Hit
TGCTTACTCTGCGTTGATACCACTG370.2713405690818422No Hit
CAGTGGTATCAACGCAGAGTACATG360.26400704018773835No Hit
GTTGATACCACTGCTTACTCTGCGT330.24200645350542682No Hit
GCAGTGGTATCAACGCAGAGTACAT310.22733939571721912No Hit
AAGCAGTGGTATCAACGCAGAGTAC300.22000586682311526No Hit
CATGGGAAGCAGTGGTATCAACGCA300.22000586682311526No Hit
GATACCACTGCTTCCCATGTACTCT290.21267233792901147No Hit
GGGAAGCAGTGGTATCAACGCAGAG290.21267233792901147No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA280.20533880903490762No Hit
CAACGCAGAGTACTTTTTTTTTTTT280.20533880903490762No Hit
GCAGAGTACATGGGAAGCAGTGGTA270.19800528014080376No Hit
GATACCACTGCTTACTCTGCGTTGA260.1906717512466999No Hit
ACATGGGAAGCAGTGGTATCAACGC260.1906717512466999No Hit
GTTGATACCACTGCTTCCCATGTAC260.1906717512466999No Hit
ACTGCTTACTCTGCGTTGATACCAC240.1760046934584922No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA230.16867116456438838No Hit
CTTCCCATGTACTCTGCGTTGATAC220.16133763567028453No Hit
TACCACTGCTTACTCTGCGTTGATA210.1540041067761807No Hit
TACCACTGCTTCCCATGTACTCTGC210.1540041067761807No Hit
CCACTGCTTCCCATGTACTCTGCGT190.13933704898797303No Hit
TGATACCACTGCTTCCCATGTACTC190.13933704898797303No Hit
ATGGGAAGCAGTGGTATCAACGCAG180.13200352009386918No Hit
TCAACGCAGAGTACTTTTTTTTTTT180.13200352009386918No Hit
GCAGTGGTATCAACGCAGAGTAAGC160.11733646230566148No Hit
CCATGTACTCTGCGTTGATACCACT160.11733646230566148No Hit
AACGCAGAGTACATGGGAAGCAGTG150.11000293341155763No Hit
AAAAAGTACTCTGCGTTGATACCAC150.11000293341155763No Hit
ATCAACGCAGAGTACATGGGAAGCA150.11000293341155763No Hit
CAGTGGTATCAACGCAGAGTAAGCA140.10266940451745381No Hit
ATACCACTGCTTACTCTGCGTTGAT140.10266940451745381No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACA1300.0031702547.3076921
GTATCAA2300.00373653795.3695651
Produced by FastQC (version 0.11.5)