FastQCFastQC Report
Thu 26 May 2016
SRR1926773_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926773_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571290
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10730.18782054648252203No Hit
GTCCTAAAGTGTGTATTTCTCATTT9890.17311698086786045No Hit
CTGTAGGACGTGGAATATGGCAAGA8630.15106163244586812No Hit
GTATCAACGCAGAGTACTTTTTTTT8460.1480859108333771No Hit
GTCCTACAGTGTGCATTTCTCATTT7100.12428013793344887No Hit
CTTTAGGACGTGAAATATGGCGAGG6850.1199040767386091No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAGA400.005196450314.28171354
TCCAACG602.6068285E-514.22419218
CTAAAGC551.8987783E-413.8672233
TAGGACC2300.013.660774
GTCCTAT801.9002164E-613.1180951
GTTTTAT751.4116582E-512.7205791
AGACTGC550.003023673812.11249356
CCAACGA550.003106242312.06901219
GGACCTG3250.012.006016
GTCCTAC6200.012.0024811
GATATAC1209.3477865E-911.9255421
TCTATAC802.7570686E-511.9171433
GTGTAGG1052.5627378E-711.8119651
ATAAGAC657.77512E-411.7338033
AGGACCT3650.011.7332385
GTCCTAA2200.011.70871451
AATGTCC1302.557499E-911.7039248
TCCTACA6450.011.6769522
ATAAGGC752.0024128E-411.4404583
AGACTGT752.0354129E-411.4203526
Produced by FastQC (version 0.11.5)