##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926773_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 571290 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.806625356648986 31.0 28.0 31.0 16.0 33.0 2 28.417633776190726 31.0 28.0 31.0 22.0 34.0 3 28.35712335241296 31.0 28.0 33.0 16.0 34.0 4 32.14444677834375 35.0 33.0 37.0 22.0 37.0 5 31.89492726986294 35.0 32.0 37.0 19.0 37.0 6 31.613411752349943 35.0 32.0 37.0 17.0 37.0 7 31.73894169336064 35.0 32.0 37.0 17.0 37.0 8 31.566657914544276 35.0 32.0 37.0 17.0 37.0 9 33.59492201858951 37.0 34.0 39.0 19.0 39.0 10 33.66888970575364 38.0 34.0 39.0 21.0 39.0 11 33.85570725901031 38.0 34.0 39.0 23.0 39.0 12 33.71181886607502 38.0 34.0 39.0 22.0 39.0 13 33.81264681685309 38.0 35.0 39.0 23.0 39.0 14 34.879660067566384 39.0 35.0 41.0 24.0 41.0 15 34.44549528260603 39.0 34.0 41.0 17.0 41.0 16 34.5459503929703 39.0 34.0 41.0 18.0 41.0 17 34.78086786045616 39.0 35.0 41.0 22.0 41.0 18 34.83371317544505 39.0 35.0 41.0 22.0 41.0 19 34.180642055698506 39.0 34.0 41.0 16.0 41.0 20 34.463174569833186 39.0 34.0 41.0 18.0 41.0 21 34.47107598592659 39.0 34.0 41.0 18.0 41.0 22 34.508232246319736 39.0 34.0 41.0 18.0 41.0 23 34.51787883561764 39.0 35.0 41.0 17.0 41.0 24 33.204031227572685 39.0 33.0 41.0 2.0 41.0 25 33.79883596772217 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.6454356608356235 1101 2 -0.7447399479905954 1101 3 -1.5921405082743938 1101 4 -0.8393801044784261 1101 5 -0.8851455346934003 1101 6 -2.391454457717959 1101 7 -0.8983544482408305 1101 8 -0.4826033074487519 1101 9 -0.47639998023543484 1101 10 -9.569853878442256 1101 11 -4.458556280253305 1101 12 -1.977121371685712 1101 13 -1.2272732310524077 1101 14 -1.1839800983894122 1101 15 -2.4320957633125104 1101 16 -0.9350561958141981 1101 17 -0.8522832408988492 1101 18 -0.7753400957600292 1101 19 -1.4783289313829187 1101 20 -0.7966944095461628 1101 21 -0.8927805533677216 1101 22 -0.7109336069410759 1101 23 -0.527628073159029 1101 24 -0.7910202157650161 1101 25 -0.6966171088711874 1102 1 -0.5597266065717186 1102 2 -0.2615205134277794 1102 3 -0.1855948624931436 1102 4 0.20729544647589648 1102 5 -0.1537846417123525 1102 6 -1.025910992625498 1102 7 -0.10450663388573034 1102 8 0.6406661838339964 1102 9 0.6359192593870162 1102 10 0.33974528232296564 1102 11 0.1335819511860592 1102 12 0.18988442145723639 1102 13 0.07315519719963248 1102 14 -0.2655962310810267 1102 15 0.3608117666719579 1102 16 0.24840388546602554 1102 17 0.01772760747719815 1102 18 -0.13656210750050235 1102 19 0.18272264521102244 1102 20 0.21561464251502827 1102 21 -0.013989128540913498 1102 22 0.1244670670900021 1102 23 -0.47459217990264335 1102 24 -3.3703895781888846 1102 25 -2.065778534909491 1103 1 -0.12285932234459196 1103 2 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2214 25 -0.21312796093783248 2215 1 0.6680413246351016 2215 2 -0.16620212856310346 2215 3 0.19796757535753073 2215 4 0.2541644086530468 2215 5 0.6563308207816583 2215 6 0.9764925438964092 2215 7 0.2613100790655416 2215 8 -0.9827411190012967 2215 9 -0.4453037877717634 2215 10 -0.39949419029011324 2215 11 -0.6210705535926735 2215 12 -0.5627367987046483 2215 13 -0.32038462216159047 2215 14 -0.30367927545757567 2215 15 -0.33204949800558836 2215 16 0.05186647221746199 2215 17 -0.6983034401349784 2215 18 -1.3807413242515807 2215 19 -0.12855336733992573 2215 20 -3.9473273583196438 2215 21 -2.179728814973423 2215 22 -1.3064911531079488 2215 23 -1.2940178042296253 2215 24 -1.2863739977077664 2215 25 -1.1605301694688919 2216 1 0.4514430786936927 2216 2 -0.5875505910106504 2216 3 -0.011324485446003507 2216 4 -0.19641118667903612 2216 5 0.08347949471144744 2216 6 -1.4476944217161964 2216 7 -10.37363903601046 2216 8 -11.146728372650802 2216 9 -5.7360703391279735 2216 10 -2.9868107581534247 2216 11 -2.1160550273511767 2216 12 -1.7348292767310731 2216 13 -1.2550416333720662 2216 14 -1.6570196900967389 2216 15 -2.4257583797812714 2216 16 -1.5269309976510996 2216 17 -1.6878884181972822 2216 18 -1.4836427457758816 2216 19 -0.7537884140621856 2216 20 -1.8366376965476903 2216 21 -4.449932067589444 2216 22 -3.146208427187979 2216 23 -2.842137191218203 2216 24 -3.36388798089445 2216 25 -2.755966315707429 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29013.0 3 6.0 4 231.0 5 1393.0 6 1730.0 7 1441.0 8 1245.0 9 1114.0 10 1124.0 11 1018.0 12 991.0 13 940.0 14 1008.0 15 1026.0 16 1067.0 17 1203.0 18 1327.0 19 1611.0 20 2088.0 21 2733.0 22 3445.0 23 4190.0 24 4888.0 25 5586.0 26 6330.0 27 7175.0 28 7774.0 29 9458.0 30 11387.0 31 13764.0 32 17973.0 33 23802.0 34 32465.0 35 46251.0 36 67978.0 37 112625.0 38 143890.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.2099913172366 18.55497767915874 12.283215955235532 25.95181504836913 2 14.27264490054874 20.475094684190324 42.19961758277966 23.052642832481276 3 18.531410313708786 25.125271443368895 30.18134783134494 26.16197041157738 4 11.331750939363948 17.021187145822566 39.65561034570345 31.991451569110037 5 12.376281827938728 38.48679084735021 36.855715678876386 12.281211645834682 6 30.916568475198847 38.1980275332961 18.85678869254486 12.028615298960192 7 26.877332294222754 32.16731786603197 23.443528201671704 17.511821638073574 8 24.04623831053947 37.790305821561965 21.888251565615434 16.27520430228313 9 26.116506384163284 15.448222010963288 20.6192804707456 37.81599113412783 10 13.798314412942563 29.717358604881657 35.94629095551038 20.538036026665406 11 33.89769679484037 22.90197547636221 25.2018543287512 17.99847340004622 12 22.529744606054663 25.442054916350955 32.750579485850736 19.277620991743643 13 28.67204240836963 22.77594151342418 26.068087281690733 22.483928796515453 14 19.861764221863524 22.86405064443503 28.42709533296685 28.847089800734597 15 22.499080898443655 32.07069203970519 23.385619999649865 22.04460706220129 16 20.95469029027627 28.970202037886484 28.880387968766414 21.194719703070835 17 20.722804240955757 29.38661605904845 28.633279177579517 21.25730052241628 18 20.635245758142336 28.401539183350486 30.51669082582299 20.446524232684187 19 23.094794969526873 27.635036828623903 27.77842947410441 21.491738727744814 20 22.668401039222964 28.256150253153034 28.663716132465915 20.41173257515809 21 22.262759666254677 27.68174449505789 27.610667805761064 22.444828032926363 22 22.169059011164276 28.981069578446668 28.02483206319361 20.82503934719545 23 21.23013788805087 28.652387655202837 28.459106283745456 21.658368173000834 24 22.61032758644837 27.633891682189326 28.822250388650016 20.933530342712288 25 21.79437576920451 28.02329103109731 28.71831052490603 21.46402267479215 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 244.0 1 244.0 2 323.0 3 402.0 4 402.0 5 402.0 6 565.0 7 728.0 8 728.0 9 728.0 10 1100.5 11 1473.0 12 1473.0 13 1473.0 14 2319.0 15 3165.0 16 3165.0 17 3165.0 18 5469.0 19 7773.0 20 7773.0 21 7773.0 22 11860.0 23 15947.0 24 15947.0 25 15947.0 26 22908.5 27 29870.0 28 29870.0 29 29870.0 30 39165.5 31 48461.0 32 48461.0 33 48461.0 34 54301.0 35 60141.0 36 60141.0 37 60141.0 38 64661.0 39 69181.0 40 69181.0 41 69181.0 42 71696.0 43 74211.0 44 74211.0 45 74211.0 46 74442.0 47 74673.0 48 74673.0 49 74673.0 50 70329.5 51 65986.0 52 65986.0 53 65986.0 54 57180.0 55 48374.0 56 48374.0 57 48374.0 58 40726.0 59 33078.0 60 33078.0 61 33078.0 62 26553.0 63 20028.0 64 20028.0 65 20028.0 66 15134.5 67 10241.0 68 10241.0 69 10241.0 70 7380.0 71 4519.0 72 4519.0 73 4519.0 74 3167.5 75 1816.0 76 1816.0 77 1816.0 78 1252.0 79 688.0 80 688.0 81 688.0 82 446.0 83 204.0 84 204.0 85 204.0 86 128.5 87 53.0 88 53.0 89 53.0 90 36.5 91 20.0 92 20.0 93 20.0 94 14.0 95 8.0 96 8.0 97 8.0 98 7.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20917572511333998 2 0.12410509548565528 3 0.12760594444152706 4 0.07439304031227573 5 0.023280645556547463 6 0.022230390869785923 7 0.2751667279315234 8 0.2706156242888901 9 0.019429711705088483 10 0.03728404138003466 11 0.015053650510248737 12 0.016453990092597456 13 0.014353480719074375 14 0.016103905197010274 15 0.014003395823487195 16 0.019254669257294896 17 0.017154159883771815 18 0.013653310927900014 19 0.022930560660960286 20 0.016453990092597456 21 0.013303226032312836 22 0.016278947644803865 23 0.017854329674946174 24 0.014353480719074375 25 0.014528523166867964 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 571290.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.32958448777369 #Duplication Level Percentage of deduplicated Percentage of total 1 93.26609975572957 80.51623638707385 2 5.182858624467217 8.948680630182583 3 0.8017068196585733 2.0763304986641744 4 0.2752134792621735 0.9503626124055184 5 0.1279326632336386 0.5522186829687151 6 0.07265062770912348 0.37631391017427407 7 0.05410795406224813 0.32697820341741995 8 0.034218555086828896 0.2363258913934347 9 0.02896102499437457 0.22501739286939462 >10 0.13108173938794074 2.110909457780226 >50 0.01238143375205207 0.7459400844815588 >100 0.011366509027847726 1.9399278396368853 >500 0.0012178402529608944 0.8067408340346647 >1k 2.0297337549348237E-4 0.18801757491728827 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1073 0.18782054648252203 No Hit GTCCTAAAGTGTGTATTTCTCATTT 989 0.17311698086786045 No Hit CTGTAGGACGTGGAATATGGCAAGA 863 0.15106163244586812 No Hit GTATCAACGCAGAGTACTTTTTTTT 846 0.1480859108333771 No Hit GTCCTACAGTGTGCATTTCTCATTT 710 0.12428013793344887 No Hit CTTTAGGACGTGAAATATGGCGAGG 685 0.1199040767386091 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.7504244779358995E-4 0.0 0.0 0.0 5 0.0 1.7504244779358995E-4 0.0 0.0 0.0 6 0.0 1.7504244779358995E-4 0.0 0.0 0.0 7 0.0 1.7504244779358995E-4 0.0 0.0 0.0 8 0.0 1.7504244779358995E-4 0.0 0.0 0.0 9 0.0 1.7504244779358995E-4 0.0 0.0 0.0 10 0.0 1.7504244779358995E-4 0.0 0.0 0.0 11 0.0 1.7504244779358995E-4 0.0 0.0 0.0 12 0.0 1.7504244779358995E-4 0.0 0.0 0.0 13 0.0 1.7504244779358995E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTAGA 40 0.0051964503 14.2817135 4 TCCAACG 60 2.6068285E-5 14.224192 18 CTAAAGC 55 1.8987783E-4 13.867223 3 TAGGACC 230 0.0 13.66077 4 GTCCTAT 80 1.9002164E-6 13.118095 1 GTTTTAT 75 1.4116582E-5 12.720579 1 AGACTGC 55 0.0030236738 12.1124935 6 CCAACGA 55 0.0031062423 12.069012 19 GGACCTG 325 0.0 12.00601 6 GTCCTAC 620 0.0 12.002481 1 GATATAC 120 9.3477865E-9 11.925542 1 TCTATAC 80 2.7570686E-5 11.917143 3 GTGTAGG 105 2.5627378E-7 11.811965 1 ATAAGAC 65 7.77512E-4 11.733803 3 AGGACCT 365 0.0 11.733238 5 GTCCTAA 220 0.0 11.7087145 1 AATGTCC 130 2.557499E-9 11.703924 8 TCCTACA 645 0.0 11.676952 2 ATAAGGC 75 2.0024128E-4 11.440458 3 AGACTGT 75 2.0354129E-4 11.420352 6 >>END_MODULE