FastQCFastQC Report
Thu 26 May 2016
SRR1926773_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926773_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571290
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14310.2504857427926272No Hit
CTGTAGGACGTGGAATATGGCAAGA10570.18501986731782458No Hit
GTCCTACAGTGGACATTTCTAAATT10300.18029372122739765No Hit
CTTTAGGACGTGAAATATGGCGAGG9650.1689159621208143No Hit
GTCCTAAAGTGTGTATTTCTCATTT9620.16839083477743352No Hit
GGTATCAACGCAGAGTACTTTTTTT8940.1564879483274694No Hit
TATCAACGCAGAGTACTTTTTTTTT8410.14721069859440916No Hit
GTCCTACAGTGTGCATTTCTCATTT6680.1169283551261181No Hit
CTGTAGGACCTGGAATATGGCGAGA5730.10029932258572703No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCGT401.2986928E-518.97303410
ATGTCGA250.00608330418.96971317
CCGTTTC401.3006555E-518.96971113
TCCAACG651.0186341E-817.51050418
GTTATAC402.6955537E-416.6743973
CTTAATC400.00517086714.292343
TAACACT801.2378041E-714.292344
TAGGGCC400.00517086714.292344
TAGGACC3000.014.292344
CTAGGAC1006.2755134E-1014.292343
TCCCGTT602.6014937E-514.22728511
CCAACGA602.6014937E-514.22728519
TATGTCG400.005329805414.227283516
GTCTTAC759.2140544E-713.9894951
CGTTTCC551.983395E-413.79615514
TAGGACT801.9277504E-613.1013124
ATAGGAC751.4303772E-512.7043033
CCCGTTT604.140331E-412.64647612
ATTACAC853.802652E-612.3306473
ATAATAC701.0588195E-412.2505783
Produced by FastQC (version 0.11.5)