##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926773_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 571290 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.850345708834393 31.0 30.0 33.0 26.0 34.0 2 30.200183794570183 31.0 30.0 34.0 26.0 34.0 3 30.302606382047646 31.0 30.0 34.0 26.0 34.0 4 33.87731099791699 37.0 35.0 37.0 30.0 37.0 5 33.84272261023298 37.0 35.0 37.0 30.0 37.0 6 33.779688074358035 37.0 35.0 37.0 30.0 37.0 7 33.742824134852704 37.0 35.0 37.0 29.0 37.0 8 33.75543769364071 37.0 35.0 37.0 29.0 37.0 9 35.28270930700695 39.0 35.0 39.0 29.0 39.0 10 35.19474347529276 39.0 35.0 39.0 29.0 39.0 11 35.22465822962068 39.0 35.0 39.0 30.0 39.0 12 35.13663813474768 39.0 35.0 39.0 29.0 39.0 13 35.139690875037196 39.0 35.0 39.0 29.0 39.0 14 36.24599590400672 40.0 36.0 41.0 29.0 41.0 15 36.23024383412977 40.0 36.0 41.0 29.0 41.0 16 36.21204992210611 40.0 36.0 41.0 29.0 41.0 17 36.17991387911569 40.0 36.0 41.0 29.0 41.0 18 36.14466908225245 40.0 36.0 41.0 29.0 41.0 19 36.209424285389204 40.0 37.0 41.0 29.0 41.0 20 36.17482364473385 40.0 37.0 41.0 29.0 41.0 21 36.12274851651525 40.0 37.0 41.0 28.0 41.0 22 36.03960685466225 40.0 37.0 41.0 28.0 41.0 23 35.98032172801904 40.0 37.0 41.0 28.0 41.0 24 35.906747886362446 40.0 36.0 41.0 27.0 41.0 25 35.79600728176583 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10591646618410167 1101 2 0.17220860597169718 1101 3 0.4918112983559908 1101 4 0.3208805250922637 1101 5 0.5785645703493003 1101 6 0.44118236838279756 1101 7 0.5414103630914369 1101 8 0.46312877507003236 1101 9 0.4949825527289917 1101 10 0.584082565021184 1101 11 0.7062332865809537 1101 12 0.8531259500016546 1101 13 0.7612739931261885 1101 14 0.7136453532841998 1101 15 0.7184008237787083 1101 16 0.7465677335666925 1101 17 0.5998575103828259 1101 18 0.8321411819094706 1101 19 0.8353812120434796 1101 20 0.7434223882576063 1101 21 0.794514569741807 1101 22 0.85382657334236 1101 23 0.8217179847023317 1101 24 0.8313226373961058 1101 25 0.6808749618590255 1102 1 -0.29423441985839105 1102 2 -0.18324228998301706 1102 3 0.03377716727236546 1102 4 -0.20714352856590068 1102 5 -0.33957424480978915 1102 6 -0.19667598596812041 1102 7 -0.2826339335262986 1102 8 -0.18040983303375668 1102 9 -0.16327845809735209 1102 10 -0.2530692681891651 1102 11 -0.15576105243905403 1102 12 -0.08202884586164316 1102 13 -0.18188492181593574 1102 14 -0.15109136709202176 1102 15 -0.24472158114656395 1102 16 -0.42463285676117835 1102 17 -0.2944878424371993 1102 18 -0.20985635104866418 1102 19 -0.17807738240347248 1102 20 -0.23998152376591975 1102 21 -0.4212455875321126 1102 22 -0.40000752910595594 1102 23 -0.33154010752538454 1102 24 -0.4531787778488905 1102 25 -0.38772311957538363 1103 1 -0.21562157403119642 1103 2 -0.08915000324053324 1103 3 0.017392197670286436 1103 4 -0.13355864110919669 1103 5 -0.19018957705061013 1103 6 -0.10626783648559268 1103 7 -0.1368383018407826 1103 8 -0.2083089375664997 1103 9 0.004437307887570796 1103 10 -0.009763220167201325 1103 11 0.1102769356608917 1103 12 -0.14575491034024424 1103 13 -0.056314142413476986 1103 14 -0.061394409089537305 1103 15 0.027174088717252687 1103 16 -0.007785061385852998 1103 17 2.817301449908882E-4 1103 18 -0.05996585524069786 1103 19 -0.0991241018949438 1103 20 -0.06378934769916356 1103 21 0.1055708795828636 1103 22 0.05119936007911008 1103 23 -0.10614753947635336 1103 24 -0.173534428720032 1103 25 -0.23243253032016753 1104 1 -0.13239571177241416 1104 2 -0.06457975647646208 1104 3 0.12979776729319425 1104 4 -0.007582882389620238 1104 5 -0.0032397109962616355 1104 6 0.1538724525853894 1104 7 -0.03961891930142514 1104 8 -1.1646361826933571E-5 1104 9 -0.19330553742223344 1104 10 -0.18765406101039872 1104 11 -0.08108186569586451 1104 12 0.013058504146854943 1104 13 0.086019500226854 1104 14 0.025561485829967978 1104 15 0.0645582951875312 1104 16 0.21250967148299793 1104 17 0.06323880986755626 1104 18 -0.014775579013821982 1104 19 0.18220371633739063 1104 20 0.16845171254230706 1104 21 0.18866961142418148 1104 22 0.09738046718845084 1104 23 0.11816032059106618 1104 24 0.30726101450640897 1104 25 -0.007523094359818572 1105 1 0.11883091751126429 1105 2 -0.09656735304494646 1105 3 0.16691028703862543 1105 4 0.1006868338348994 1105 5 -0.07016592530846566 1105 6 -0.23135771182865028 1105 7 -0.5538356288874198 1105 8 -0.39537991450350063 1105 9 -0.3602426720365415 1105 10 -0.38758447582950595 1105 11 -0.40377171387827104 1105 12 -0.3407770614994021 1105 13 -0.4264677382573083 1105 14 -0.3157093833752924 1105 15 -0.29207845493056084 1105 16 -0.44796543784700305 1105 17 -0.3109059781008128 1105 18 -0.20380854816777827 1105 19 -0.39402761121579744 1105 20 -0.21075069906673605 1105 21 -0.47590949981560016 1105 22 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1108 22 0.7880235888530365 1108 23 0.8632307141891289 1108 24 0.8638519714587076 1108 25 0.9692259592687549 1109 1 0.14539271879102245 1109 2 0.14702419511046116 1109 3 0.16437543121399756 1109 4 0.4050314646116604 1109 5 0.21269907213176964 1109 6 0.3934910999031871 1109 7 0.3273525022574262 1109 8 0.32680985215274205 1109 9 0.2649300033387334 1109 10 0.14850950441730504 1109 11 0.0899260241833062 1109 12 0.12695553176398988 1109 13 0.17617274694025298 1109 14 0.29623470779481664 1109 15 0.16770453151574571 1109 16 0.32648595582544715 1109 17 0.4149085701031794 1109 18 0.3601700917793096 1109 19 0.11985679686733874 1109 20 0.34108180862190807 1109 21 0.4792503331205822 1109 22 0.516597410742321 1109 23 0.5021080213790299 1109 24 0.20292464896416362 1109 25 0.2814430911418242 1110 1 0.49306041073760554 1110 2 0.5448148717463042 1110 3 0.6093196113242207 1110 4 0.6188353345950901 1110 5 0.4985797940983474 1110 6 0.7091754375707282 1110 7 0.789447300714123 1110 8 0.6816646910990372 1110 9 0.7587991065529636 1110 10 0.46349945537012616 1110 11 0.5363983440706406 1110 12 0.5497526877124841 1110 13 0.8117627555956375 1110 14 0.5844137519861761 1110 15 0.7146599880750131 1110 16 0.689598862127248 1110 17 0.6884407001588784 1110 18 0.5396849501879899 1110 19 0.5613437135458241 1110 20 0.607595498309287 1110 21 0.6264774318066273 1110 22 0.6823039981250218 1110 23 0.6246856142043526 1110 24 0.6873730760804548 1110 25 0.6433163704564393 1111 1 0.42899625550725773 1111 2 0.1415725975526776 1111 3 0.45728048737098703 1111 4 0.5457367673504905 1111 5 0.5827463848690826 1111 6 0.6588947824050919 1111 7 0.6615299010525391 1111 8 0.49393850125056105 1111 9 0.4130192399061059 1111 10 0.39895356070090315 1111 11 0.49881427934019484 1111 12 0.5183114000061906 1111 13 0.4974445487209067 1111 14 0.4933661963944047 1111 15 0.40586620610884694 1111 16 0.682473432516332 1111 17 0.7919427781095933 1111 18 0.6224638437691112 1111 19 0.6885217270537041 1111 20 0.8347236601737436 1111 21 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2214 8 0.17332574153433455 2214 9 0.29160328318266693 2214 10 0.042577531757032716 2214 11 0.3952085954623854 2214 12 0.36038490610849294 2214 13 0.50738994246381 2214 14 0.22782383535258788 2214 15 0.34454033926642325 2214 16 0.39250330002711564 2214 17 0.3324762624436488 2214 18 0.5085333304053137 2214 19 0.2673109209093951 2214 20 0.3914406011094016 2214 21 0.39300992439781623 2214 22 0.5436092410693405 2214 23 0.31077136251717974 2214 24 0.3165840955880128 2214 25 0.4814258084947838 2215 1 -0.23466509856764262 2215 2 -0.2552582208188703 2215 3 -0.19473140353793283 2215 4 -0.5010177306072308 2215 5 -0.32047862372873936 2215 6 -0.509732109705169 2215 7 -0.6406977599152199 2215 8 -0.5118338189371343 2215 9 -0.7604954564846906 2215 10 -0.5120754457674934 2215 11 -0.7662142465922557 2215 12 -0.4810867522860178 2215 13 -0.6344814110554111 2215 14 -0.4220005891499454 2215 15 -0.47525886043596444 2215 16 -0.7239504378708759 2215 17 -0.638522022632884 2215 18 -0.5771788165155414 2215 19 -0.7802328368599376 2215 20 -0.9916791985439843 2215 21 -0.9338145636204018 2215 22 -1.0567977129906723 2215 23 -0.8582688247633499 2215 24 -0.7418208441174627 2215 25 -0.8207886204538468 2216 1 -0.7976612086341 2216 2 -0.43750854497999825 2216 3 -2.522150889569101 2216 4 -1.8570315774309307 2216 5 -0.8499509707510029 2216 6 -0.6471919783182329 2216 7 -0.5557304631080271 2216 8 -0.45375395329055834 2216 9 -0.6068873397771171 2216 10 -0.6065618357847669 2216 11 -0.6840124867920636 2216 12 -0.586527635343991 2216 13 -0.6055246118056488 2216 14 -0.6157963126153874 2216 15 -0.5687093702421322 2216 16 -0.7171645851716093 2216 17 -0.7660704554155018 2216 18 -0.5183262272934783 2216 19 -0.7088181067868717 2216 20 -0.7972291719890876 2216 21 -0.4833573887743512 2216 22 -0.5896432496148947 2216 23 -0.7588039896668732 2216 24 -0.7865562760053422 2216 25 -0.7558355819516365 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14020.0 3 8.0 4 231.0 5 1536.0 6 2142.0 7 1567.0 8 1191.0 9 1054.0 10 929.0 11 840.0 12 785.0 13 724.0 14 680.0 15 753.0 16 788.0 17 763.0 18 849.0 19 1003.0 20 1345.0 21 1678.0 22 2176.0 23 2674.0 24 3131.0 25 3655.0 26 4547.0 27 4939.0 28 5713.0 29 6859.0 30 8789.0 31 11431.0 32 14936.0 33 19491.0 34 26875.0 35 37750.0 36 55611.0 37 97366.0 38 232461.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.86816826763829 19.55551349390369 10.134229246518084 28.442088991939933 2 13.99532157594554 21.531789628617236 40.17434576532534 24.29854303011188 3 17.949997286457318 25.694448091679227 29.682305425859884 26.673249196003564 4 11.661327872008963 17.900015753820302 38.783630030282346 31.655026343888395 5 12.20646593298102 39.25282396620868 36.05154121248935 12.489168888320954 6 30.661350102759577 39.037397459994025 18.155597322981258 12.145655114265137 7 27.36425979064503 32.3862930094208 22.528156475245236 17.721290724688934 8 24.508744995544788 38.52647131420889 20.22806486229105 16.736718827955276 9 26.186799990196803 15.549276838887897 20.082556954845757 38.18136621606955 10 13.793725175793456 30.1853599105147 35.08114419352072 20.93977072017113 11 33.79015416609047 23.055769018690032 24.946828715819397 18.207248099400104 12 22.71716793339938 25.59506096771144 32.33634291755793 19.351428181331244 13 28.631068555974686 22.805954996192792 25.91141024182326 22.65156620600926 14 20.16874091967302 22.98587407446306 28.132297082042395 28.71308792382153 15 22.18785501355725 32.04937675154609 23.508921178894447 22.25384705600221 16 21.17670947052975 29.14834549299127 28.4100138634104 21.264931173068575 17 20.699119536487597 29.47049659542439 28.40886414955627 21.421519718531744 18 20.591473607743946 28.43799504625537 30.4681551239749 20.502376222025784 19 22.68917712545292 27.591766003255792 28.064380612298486 21.654676258992804 20 22.86358448687518 28.125765818752146 28.627267698727778 20.383381995644896 21 22.01461770755832 27.4692109063854 27.86467679110683 22.65149459494945 22 22.294281363230585 28.937667384340703 27.772234766930982 20.995816485497734 23 21.297001332080022 28.62624215149303 28.426342626385686 21.65041389004126 24 22.266799698926988 27.703618127395895 29.02641390537205 21.003168268305064 25 21.832927505991133 28.10886792551916 28.5344177179958 21.523786850493906 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 207.0 1 207.0 2 216.0 3 225.0 4 225.0 5 225.0 6 421.0 7 617.0 8 617.0 9 617.0 10 945.0 11 1273.0 12 1273.0 13 1273.0 14 2111.5 15 2950.0 16 2950.0 17 2950.0 18 5039.5 19 7129.0 20 7129.0 21 7129.0 22 11367.0 23 15605.0 24 15605.0 25 15605.0 26 22327.0 27 29049.0 28 29049.0 29 29049.0 30 38281.5 31 47514.0 32 47514.0 33 47514.0 34 53848.5 35 60183.0 36 60183.0 37 60183.0 38 63964.0 39 67745.0 40 67745.0 41 67745.0 42 70599.0 43 73453.0 44 73453.0 45 73453.0 46 74831.0 47 76209.0 48 76209.0 49 76209.0 50 71997.5 51 67786.0 52 67786.0 53 67786.0 54 58594.0 55 49402.0 56 49402.0 57 49402.0 58 41618.5 59 33835.0 60 33835.0 61 33835.0 62 27020.5 63 20206.0 64 20206.0 65 20206.0 66 15373.5 67 10541.0 68 10541.0 69 10541.0 70 7632.0 71 4723.0 72 4723.0 73 4723.0 74 3266.5 75 1810.0 76 1810.0 77 1810.0 78 1195.0 79 580.0 80 580.0 81 580.0 82 387.5 83 195.0 84 195.0 85 195.0 86 118.5 87 42.0 88 42.0 89 42.0 90 25.5 91 9.0 92 9.0 93 9.0 94 5.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12235467100771936 2 0.10239983195925012 3 0.014178438271280786 4 0.0 5 0.003325806508078209 6 0.35008489558717987 7 0.018729541913914124 8 0.008577079941885907 9 0.008402037494092317 10 0.004025976299252569 11 0.004025976299252569 12 7.001697911743598E-4 13 8.752122389679497E-4 14 0.0 15 0.0015753820301423094 16 3.500848955871799E-4 17 0.0 18 8.752122389679497E-4 19 0.0 20 0.0010502546867615396 21 0.011377759106583346 22 0.0 23 5.251273433807698E-4 24 0.0 25 0.005076230986014108 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 571290.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.81139832484537 #Duplication Level Percentage of deduplicated Percentage of total 1 91.70394671697855 76.85836006257084 2 6.4064495482817465 10.738669898781342 3 1.0227155893920068 2.571456709066874 4 0.3108910051854196 1.0422483948482708 5 0.1599920773535208 0.6704579861947702 6 0.09021677087835593 0.4536716231800319 7 0.05996011736538318 0.3517738896080225 8 0.03632947161589148 0.24358590532245153 9 0.024783492717166834 0.1869425261999436 >10 0.15540518033842088 2.392505300736548 >50 0.013398898599270879 0.7534491426406521 >100 0.013817561387240712 2.1659402242386405 >500 0.0014654989349455788 0.9536523913195636 >1k 6.280709721195337E-4 0.6172859452920376 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1431 0.2504857427926272 No Hit CTGTAGGACGTGGAATATGGCAAGA 1057 0.18501986731782458 No Hit GTCCTACAGTGGACATTTCTAAATT 1030 0.18029372122739765 No Hit CTTTAGGACGTGAAATATGGCGAGG 965 0.1689159621208143 No Hit GTCCTAAAGTGTGTATTTCTCATTT 962 0.16839083477743352 No Hit GGTATCAACGCAGAGTACTTTTTTT 894 0.1564879483274694 No Hit TATCAACGCAGAGTACTTTTTTTTT 841 0.14721069859440916 No Hit GTCCTACAGTGTGCATTTCTCATTT 668 0.1169283551261181 No Hit CTGTAGGACCTGGAATATGGCGAGA 573 0.10029932258572703 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCCGT 40 1.2986928E-5 18.973034 10 ATGTCGA 25 0.006083304 18.969713 17 CCGTTTC 40 1.3006555E-5 18.969711 13 TCCAACG 65 1.0186341E-8 17.510504 18 GTTATAC 40 2.6955537E-4 16.674397 3 CTTAATC 40 0.005170867 14.29234 3 TAACACT 80 1.2378041E-7 14.29234 4 TAGGGCC 40 0.005170867 14.29234 4 TAGGACC 300 0.0 14.29234 4 CTAGGAC 100 6.2755134E-10 14.29234 3 TCCCGTT 60 2.6014937E-5 14.227285 11 CCAACGA 60 2.6014937E-5 14.227285 19 TATGTCG 40 0.0053298054 14.2272835 16 GTCTTAC 75 9.2140544E-7 13.989495 1 CGTTTCC 55 1.983395E-4 13.796155 14 TAGGACT 80 1.9277504E-6 13.101312 4 ATAGGAC 75 1.4303772E-5 12.704303 3 CCCGTTT 60 4.140331E-4 12.646476 12 ATTACAC 85 3.802652E-6 12.330647 3 ATAATAC 70 1.0588195E-4 12.250578 3 >>END_MODULE