FastQCFastQC Report
Thu 26 May 2016
SRR1926772_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926772_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329495
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT6560.19909255072155874No Hit
GTACATGGGAAGCAGTGGTATCAAC5990.18179335043020378No Hit
CCCATGTACTCTGCGTTGATACCAC5350.16236968694517367No Hit
GGTATCAACGCAGAGTACTTTTTTT3850.11684547565213432No Hit
TATCAACGCAGAGTACTTTTTTTTT3710.11259654926478399No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGTC307.5247133E-419.0669923
TAGGGGA350.002127947316.3281864
TAGGACG508.489855E-515.2396414
GAATTAG400.005114570714.313351
GGACGTG400.005203376514.2762796
AAAGCTG400.005203376514.2762795
GTCCAAG500.001445985313.3591271
TGGAGTT500.001474539613.3245285
AGGACGT500.001474539613.3245285
GTCTATA550.002959572212.144661
GTCTTAG802.7236942E-511.9277921
GTACTGG600.00566857211.1326061
GTAGGAC803.6334532E-410.7251833
TACAACT803.6643384E-410.7153724
ACAACTG803.690253E-410.707215
CTATTCT750.002581664610.159764
CTGTCCA750.002671216110.1181099
GTATACA1053.8833747E-59.9966251
GTATCAA4700.09.5422331
GTATATG900.00106449169.5422331
Produced by FastQC (version 0.11.5)