FastQCFastQC Report
Thu 26 May 2016
SRR1926772_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926772_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329495
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13380.406075964733911No Hit
GGTATCAACGCAGAGTACTTTTTTT8370.2540250990151596No Hit
TATCAACGCAGAGTACTTTTTTTTT6760.20516244556063068No Hit
CCCATGTACTCTGCGTTGATACCAC6730.20425196133476986No Hit
GTACATGGGAAGCAGTGGTATCAAC6720.20394846659281626No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4300.13050273904004614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACA704.296635E-714.9614734
CTAGGAC400.005191000614.2814063
GTATAAG500.001463816713.3374381
AGACTGA500.001470557513.3293136
GTGCTAT603.9812227E-412.7023211
GTCCACT550.00308593112.07709911
AATGTCC550.00308593112.0770998
TAAAGAG802.7867614E-511.9011735
ACACAGT855.1756568E-511.2011046
AAGACTG600.005763713311.107765
CTTACAC600.005763713311.107763
GCTCACA600.005909073611.07067311
TGCACCA700.001500241110.84474110
AGACAAT750.002590325710.1556666
CATGACT800.00441847379.5209384
AGGACAG850.00727616538.9608835
ACTGTGT1000.00292201488.5402348
AGTGGTA2606.002665E-118.39424714
GAAGCAG2752.1827873E-118.2814399
GGGAAGC2658.913048E-118.2396167
Produced by FastQC (version 0.11.5)