FastQCFastQC Report
Thu 26 May 2016
SRR1926771_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926771_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525059
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8290.15788701841126426No Hit
GTATCAACGCAGAGTACTTTTTTTT7890.15026882693183052No Hit
CTGTAGGACGTGGAATATGGCAAGA7440.14169836151746756No Hit
GTCCTAAAGTGTGTATTTCTCATTT7410.14112699715651003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACC456.5888E-414.8254544
GATTAAT400.00510913514.3179141
CTACCCT551.9035037E-413.86276154
AGGACTG1204.9112714E-1113.4927055
GTATTAA655.201623E-513.2165361
GTCATAA603.9233483E-412.7270351
GTATTAT603.9233483E-412.7270351
GTTTTAT905.0803646E-712.7270351
TTAAGAC603.948834E-412.7172763
TAGGACC1457.2759576E-1212.4884364
GTCTTAG853.7257087E-612.352711
TGACCTG550.003005784212.1217895
ACCTTTT951.0648127E-611.97220815
GTAGGAC3300.011.8501883
TGTAGGA3550.011.8216922
GGACTGC657.838247E-411.722176
CTGTAGG3700.011.6091191
TAGGACT752.0078124E-411.4367784
GTCCTAA3050.011.2665551
AGGACGT3300.011.2559475
Produced by FastQC (version 0.11.5)