##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926771_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 525059 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.053494940568584 31.0 28.0 31.0 22.0 33.0 2 28.647696735033588 31.0 30.0 31.0 23.0 34.0 3 28.5736155365397 31.0 28.0 33.0 16.0 34.0 4 32.35098150874473 35.0 33.0 37.0 24.0 37.0 5 32.19573800277683 35.0 33.0 37.0 22.0 37.0 6 31.840473165872787 35.0 32.0 37.0 17.0 37.0 7 31.92641398395228 35.0 32.0 37.0 17.0 37.0 8 31.74020062507261 35.0 32.0 37.0 17.0 37.0 9 33.7883685452492 38.0 34.0 39.0 22.0 39.0 10 33.887791657699424 38.0 34.0 39.0 23.0 39.0 11 34.0566469672932 38.0 35.0 39.0 25.0 39.0 12 33.89973126829556 38.0 34.0 39.0 23.0 39.0 13 34.02557426879646 38.0 35.0 39.0 25.0 39.0 14 35.11673354803936 39.0 36.0 41.0 25.0 41.0 15 34.66190847123847 39.0 34.0 41.0 19.0 41.0 16 34.76867552027486 39.0 34.0 41.0 22.0 41.0 17 35.02568092347717 39.0 35.0 41.0 25.0 41.0 18 35.08072045236821 39.0 36.0 41.0 25.0 41.0 19 34.430709691672746 39.0 34.0 41.0 18.0 41.0 20 34.69184225010903 39.0 34.0 41.0 19.0 41.0 21 34.683062284428985 39.0 35.0 41.0 19.0 41.0 22 34.743897352488005 39.0 35.0 41.0 19.0 41.0 23 34.753886706065416 39.0 35.0 41.0 19.0 41.0 24 33.44244170655107 39.0 33.0 41.0 2.0 41.0 25 34.036662546494775 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.23167755971002535 1101 2 -0.2186750396832089 1101 3 -0.847315138089872 1101 4 -0.20186003730495372 1101 5 -0.2873720023641333 1101 6 -1.629064464021674 1101 7 -0.16363558313775073 1101 8 0.07255064259448929 1101 9 -0.08451103687772132 1101 10 -9.43272884730452 1101 11 -4.0454049959000855 1101 12 -1.716658837853437 1101 13 -0.7341097785339556 1101 14 -0.6078425604805773 1101 15 -1.9036186531403843 1101 16 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-6.151297124353462 2216 10 -3.497365360216893 2216 11 -2.2415536748073848 2216 12 -1.7954019338379155 2216 13 -1.6685925371546446 2216 14 -1.878420258644482 2216 15 -2.7012899473633993 2216 16 -1.767464337722167 2216 17 -2.079990572101387 2216 18 -1.767359374295559 2216 19 -0.971361184723591 2216 20 -1.8990992959351658 2216 21 -4.382138163124072 2216 22 -2.803261092613724 2216 23 -2.4840284722256456 2216 24 -3.1265114772546276 2216 25 -2.3290205603738166 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24738.0 3 7.0 4 240.0 5 1158.0 6 1626.0 7 1340.0 8 1027.0 9 991.0 10 881.0 11 858.0 12 856.0 13 822.0 14 875.0 15 881.0 16 945.0 17 1010.0 18 1170.0 19 1460.0 20 1907.0 21 2527.0 22 2991.0 23 3665.0 24 4300.0 25 4996.0 26 5786.0 27 6440.0 28 7126.0 29 8620.0 30 10395.0 31 12637.0 32 16227.0 33 21708.0 34 29493.0 35 41601.0 36 61203.0 37 101582.0 38 140970.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.38973345930316 18.608606029373874 12.244457804880376 25.757202706442595 2 14.541359720969439 20.419651898311745 41.548131437278194 23.490856943440626 3 18.677890208133892 24.825601574448772 29.998817637792875 26.497690579624468 4 11.605750942418155 16.858517211280812 38.88975708671772 32.64597475958332 5 12.704544848422506 38.11249157056154 36.51670838524343 12.66625519577252 6 31.64801712938093 37.67435126660647 18.441325393469043 12.236306210543562 7 27.228239268680444 32.02064479638009 23.011381007704536 17.739734927234927 8 24.439192429456032 37.33781431685699 21.68545100564199 16.537542248044986 9 26.072305497297926 15.484401876689391 20.45823832736153 37.98505429865116 10 13.976577101802157 29.87172086093535 35.30715166202743 20.84455037523506 11 34.068475250391444 23.026869697396844 24.76351389963008 18.141141152581632 12 22.444944368143645 25.39045162666748 32.43239124595929 19.732212759229594 13 28.46380521887542 22.58680823423413 26.177237565693108 22.772148981197343 14 20.18008716721781 22.570328096188717 28.09371237865927 29.1558723579342 15 22.790011048039926 31.537201417196847 23.28679187778582 22.385995656977407 16 21.437973487554313 29.034156823078284 28.218286691773294 21.309582997594106 17 20.981328172324854 29.340218911083088 28.345099264327704 21.333353652264357 18 20.9471544792879 28.50968507551673 29.885729633150028 20.657430812045344 19 23.133266405176965 27.760453079488933 27.718734105356603 21.3875464099775 20 22.810870737129243 28.294242136202328 28.29881383472167 20.596073291946762 21 22.387920393764382 27.60007924051019 27.70979686999985 22.302203495725585 22 22.24427635050317 28.630152903684202 27.90211268812944 21.223458057683185 23 21.57301615723685 28.220494279629538 28.269068853982926 21.937420709150686 24 22.664949769323666 27.60172807241446 28.585959792600836 21.14736236566104 25 22.15032544034927 27.74386120640557 28.351121278674846 21.75469207457032 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 231.0 1 231.0 2 303.5 3 376.0 4 376.0 5 376.0 6 536.0 7 696.0 8 696.0 9 696.0 10 989.5 11 1283.0 12 1283.0 13 1283.0 14 2094.5 15 2906.0 16 2906.0 17 2906.0 18 4983.5 19 7061.0 20 7061.0 21 7061.0 22 10718.5 23 14376.0 24 14376.0 25 14376.0 26 20478.5 27 26581.0 28 26581.0 29 26581.0 30 34710.5 31 42840.0 32 42840.0 33 42840.0 34 48582.0 35 54324.0 36 54324.0 37 54324.0 38 58094.5 39 61865.0 40 61865.0 41 61865.0 42 63992.0 43 66119.0 44 66119.0 45 66119.0 46 66922.0 47 67725.0 48 67725.0 49 67725.0 50 64050.5 51 60376.0 52 60376.0 53 60376.0 54 53156.5 55 45937.0 56 45937.0 57 45937.0 58 39126.0 59 32315.0 60 32315.0 61 32315.0 62 26294.0 63 20273.0 64 20273.0 65 20273.0 66 15689.0 67 11105.0 68 11105.0 69 11105.0 70 8226.5 71 5348.0 72 5348.0 73 5348.0 74 3757.0 75 2166.0 76 2166.0 77 2166.0 78 1475.5 79 785.0 80 785.0 81 785.0 82 521.0 83 257.0 84 257.0 85 257.0 86 169.0 87 81.0 88 81.0 89 81.0 90 53.0 91 25.0 92 25.0 93 25.0 94 15.0 95 5.0 96 5.0 97 5.0 98 4.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2140711805720881 2 0.12798561685448684 3 0.13046152908530279 4 0.065706901510116 5 0.021521390929400315 6 0.021521390929400315 7 0.3296772362724951 8 0.3165358559704719 9 0.0175218404026976 10 0.03580549995333858 11 0.015426837745853322 12 0.017140930828725913 13 0.01523638295886748 14 0.017331385615711757 15 0.015045928171881636 16 0.018664569124612663 17 0.01847411433762682 18 0.014474563810924107 19 0.022473664864329532 20 0.017140930828725913 21 0.01428410902393826 22 0.016760021254754228 23 0.017712295189683445 24 0.015426837745853322 25 0.014855473384895793 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 525059.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.5074359898481 #Duplication Level Percentage of deduplicated Percentage of total 1 91.95577155600928 78.62902250222558 2 6.20268985012152 10.607522106482927 3 1.0156733374139586 2.6054286865655834 4 0.3250298865399809 1.1116988887242003 5 0.1490798926791054 0.6373719690316014 6 0.08518876502773078 0.437056372359774 7 0.05098821725244711 0.30519102060650627 8 0.03577829820967894 0.24474484351918552 9 0.024492551550635108 0.1884865755549588 >10 0.13189729797518482 2.063836684857032 >50 0.011593705192434495 0.711291902191183 >100 0.010924819988209879 1.8667815433628192 >500 8.918220398514909E-4 0.5915669045186647 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 829 0.15788701841126426 No Hit GTATCAACGCAGAGTACTTTTTTTT 789 0.15026882693183052 No Hit CTGTAGGACGTGGAATATGGCAAGA 744 0.14169836151746756 No Hit GTCCTAAAGTGTGTATTTCTCATTT 741 0.14112699715651003 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.904547869858435E-4 0.0 0.0 0.0 3 0.0 1.904547869858435E-4 0.0 0.0 0.0 4 0.0 1.904547869858435E-4 0.0 1.904547869858435E-4 0.0 5 0.0 1.904547869858435E-4 0.0 1.904547869858435E-4 0.0 6 0.0 1.904547869858435E-4 0.0 1.904547869858435E-4 0.0 7 0.0 1.904547869858435E-4 0.0 1.904547869858435E-4 0.0 8 0.0 1.904547869858435E-4 0.0 1.904547869858435E-4 0.0 9 0.0 1.904547869858435E-4 0.0 3.80909573971687E-4 0.0 10 0.0 1.904547869858435E-4 0.0 3.80909573971687E-4 0.0 11 0.0 1.904547869858435E-4 0.0 9.522739349292175E-4 0.0 12 0.0 1.904547869858435E-4 0.0 9.522739349292175E-4 0.0 13 0.0 1.904547869858435E-4 0.0 9.522739349292175E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATACC 45 6.5888E-4 14.825454 4 GATTAAT 40 0.005109135 14.317914 1 CTACCCT 55 1.9035037E-4 13.8627615 4 AGGACTG 120 4.9112714E-11 13.492705 5 GTATTAA 65 5.201623E-5 13.216536 1 GTCATAA 60 3.9233483E-4 12.727035 1 GTATTAT 60 3.9233483E-4 12.727035 1 GTTTTAT 90 5.0803646E-7 12.727035 1 TTAAGAC 60 3.948834E-4 12.717276 3 TAGGACC 145 7.2759576E-12 12.488436 4 GTCTTAG 85 3.7257087E-6 12.35271 1 TGACCTG 55 0.0030057842 12.121789 5 ACCTTTT 95 1.0648127E-6 11.972208 15 GTAGGAC 330 0.0 11.850188 3 TGTAGGA 355 0.0 11.821692 2 GGACTGC 65 7.838247E-4 11.72217 6 CTGTAGG 370 0.0 11.609119 1 TAGGACT 75 2.0078124E-4 11.436778 4 GTCCTAA 305 0.0 11.266555 1 AGGACGT 330 0.0 11.255947 5 >>END_MODULE