FastQCFastQC Report
Thu 26 May 2016
SRR1926771_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926771_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525059
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13580.25863760072677544No Hit
CTGTAGGACGTGGAATATGGCAAGA8630.16436248116878294No Hit
GGTATCAACGCAGAGTACTTTTTTT8610.16398157159481125No Hit
GTCCTACAGTGGACATTTCTAAATT8480.16150565936399527No Hit
TATCAACGCAGAGTACTTTTTTTTT7580.14436472853526935No Hit
GTCCTAAAGTGTGTATTTCTCATTT7100.13522289875994886No Hit
CTTTAGGACGTGAAATATGGCGAGG6330.12055788016203893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGCG351.0021188E-418.9758119
TCCAACG456.8222353E-414.75755818
TAGGACC1550.014.7485394
CACTGTT1251.4260877E-912.1456767
ACTGTTC1353.765308E-1011.9477338
TTAGACA657.832509E-411.7231984
GATATAC2000.011.4399611
GTAGGAC3700.011.3282273
ACATATC600.005748633811.1136773
TACCAAT600.005752728311.1126156
GTAGACT600.005752728311.1126154
AAGCTTC600.005752728311.1126156
TGTAGGA3950.011.0999482
TAGACAG700.0014561310.8858265
TTACACA700.0014561310.8858264
GTCCTAC4050.010.8279471
AAATTTT1158.928564E-710.72443716
CTAGGAC803.667814E-410.716763
TAGGACT803.671058E-410.7157354
GGACAGT803.671058E-410.7157356
Produced by FastQC (version 0.11.5)