##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926771_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 525059 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.837802989759247 31.0 30.0 33.0 26.0 34.0 2 30.114299535861683 31.0 30.0 33.0 26.0 34.0 3 30.145878844091808 31.0 30.0 34.0 26.0 34.0 4 33.873890362797326 35.0 35.0 37.0 30.0 37.0 5 33.888456344906004 37.0 35.0 37.0 30.0 37.0 6 33.866630226317426 37.0 35.0 37.0 30.0 37.0 7 33.8467657920348 37.0 35.0 37.0 30.0 37.0 8 33.88089719441053 37.0 35.0 37.0 30.0 37.0 9 35.3941461816672 39.0 35.0 39.0 30.0 39.0 10 35.28897133465001 39.0 35.0 39.0 30.0 39.0 11 35.35346123007129 39.0 35.0 39.0 30.0 39.0 12 35.25439998171634 39.0 35.0 39.0 30.0 39.0 13 35.26539112747329 39.0 35.0 39.0 30.0 39.0 14 36.35032443972963 40.0 36.0 41.0 30.0 41.0 15 36.336520276768894 40.0 36.0 41.0 29.0 41.0 16 36.31982310559385 40.0 36.0 41.0 29.0 41.0 17 36.309363328692584 40.0 36.0 41.0 29.0 41.0 18 36.27274268225095 40.0 36.0 41.0 29.0 41.0 19 36.34479172816769 40.0 37.0 41.0 29.0 41.0 20 36.32046303367812 40.0 37.0 41.0 29.0 41.0 21 36.268727895341286 40.0 37.0 41.0 29.0 41.0 22 36.18258900428333 40.0 37.0 41.0 29.0 41.0 23 36.12746567528602 40.0 37.0 41.0 29.0 41.0 24 36.04973726762135 40.0 37.0 41.0 29.0 41.0 25 35.94085997954516 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0644204370482413 1101 2 0.05599457658536622 1101 3 0.2203815457839795 1101 4 0.08561290301659596 1101 5 -4.856715306900128E-4 1101 6 0.04751033843461272 1101 7 0.10082328215367653 1101 8 0.12129392853469767 1101 9 0.24379776851250057 1101 10 0.26338257061257764 1101 11 0.18424503934468106 1101 12 0.13701142979930836 1101 13 0.37155842109493875 1101 14 0.31028083103510795 1101 15 0.15986624817880113 1101 16 0.3094369432613817 1101 17 0.20825172855498408 1101 18 0.2788778114258079 1101 19 0.08637004523927772 1101 20 0.2261936143035328 1101 21 0.013013869057630245 1101 22 0.04271392654162298 1101 23 0.009234265022740828 1101 24 0.15312515279228478 1101 25 -0.025751629156729905 1102 1 -0.14606914785807135 1102 2 -0.19582031957040869 1102 3 0.02361705860670682 1102 4 -0.0921414331931345 1102 5 0.13530210926374764 1102 6 -0.1447095588452072 1102 7 -0.021235617668203588 1102 8 0.14542625426489053 1102 9 0.4327371912734961 1102 10 0.15900226383546823 1102 11 0.3678271030865332 1102 12 0.4474390847009957 1102 13 0.25456808785959595 1102 14 0.2606240962918207 1102 15 0.2602345882275685 1102 16 0.11963806038659897 1102 17 0.2178268405268966 1102 18 0.13540503919138303 1102 19 0.003362107770620071 1102 20 0.20839742913051396 1102 21 0.0499308801424192 1102 22 0.06263368720705387 1102 23 -0.008357863221853279 1102 24 0.07132080534213259 1102 25 -0.004396552808309195 1103 1 -1.037147709775514 1103 2 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0.5168431985682105 2210 1 -0.17008040208320807 2210 2 -0.05629001382422416 2210 3 0.1631793729568045 2210 4 0.057500390529078516 2210 5 -0.04543447272948953 2210 6 -0.12745532090605138 2210 7 -0.206659859704466 2210 8 -0.10755534718957449 2210 9 -0.1165512574615235 2210 10 -0.11957821859821394 2210 11 -0.11167778782814253 2210 12 -0.2479286301407555 2210 13 -0.32509492555841035 2210 14 -0.2780214916422139 2210 15 -0.2170537068661531 2210 16 -0.1853495202750821 2210 17 -0.28604460011634814 2210 18 -0.3710222884742933 2210 19 -0.4283683413741102 2210 20 -0.4297004915905731 2210 21 -0.22698675531798784 2210 22 -0.3733731114204204 2210 23 -0.33343244326896837 2210 24 -0.20251920285206637 2210 25 -0.09649650927661213 2211 1 -0.10093667860045485 2211 2 0.05632470209534901 2211 3 0.17679252089999054 2211 4 0.002474219158486335 2211 5 -0.0630569132386185 2211 6 -0.1988068934941296 2211 7 -0.30775686671424296 2211 8 -0.2185329112921437 2211 9 -0.16275179702986975 2211 10 -0.2387040717618376 2211 11 -0.12300526430849601 2211 12 -0.08003986423040743 2211 13 -0.3093690447246189 2211 14 -0.12833451045793254 2211 15 -0.07944360264184525 2211 16 -0.1221865890815792 2211 17 -0.17343564881584683 2211 18 -0.38167530452299303 2211 19 -0.23877866695979577 2211 20 -0.3683188276909206 2211 21 -0.16957979626984354 2211 22 -0.25789337982108407 2211 23 0.006365910355825122 2211 24 -0.2370146833907114 2211 25 -0.40315983246421183 2212 1 0.16861930291191385 2212 2 -0.0025278698263520027 2212 3 0.25970058890925074 2212 4 0.26268266140122876 2212 5 0.08639999182555869 2212 6 0.1731488291250045 2212 7 0.11397356900639011 2212 8 0.15170930324072884 2212 9 0.08610517275761964 2212 10 -0.03669681943220837 2212 11 -0.1172424005510635 2212 12 0.21728075678143455 2212 13 0.022101419042776627 2212 14 -0.01829658923781352 2212 15 -0.024528365281554443 2212 16 0.13110143793625895 2212 17 0.023675866124449385 2212 18 -0.17146146867126077 2212 19 -0.07881667115851343 2212 20 0.04476938685854748 2212 21 0.06234953915454611 2212 22 0.14845697242307665 2212 23 0.24195045310224828 2212 24 0.08241558554292538 2212 25 -0.06852788404371069 2213 1 0.3978059939281877 2213 2 0.3149261542669457 2213 3 0.6118528060052384 2213 4 0.5363554475091377 2213 5 0.33633190903689325 2213 6 0.4650037246779988 2213 7 0.4541752542556523 2213 8 0.3555411627819325 2213 9 0.29722189068662175 2213 10 0.1667465677265767 2213 11 0.4066626680123093 2213 12 0.438770087807967 2213 13 0.3506529376894534 2213 14 0.21552373627801558 2213 15 0.508952347264902 2213 16 0.21609278741722449 2213 17 0.38600846256171906 2213 18 0.39153113032912756 2213 19 0.4233499634691995 2213 20 0.4095089101188307 2213 21 0.32629850109226766 2213 22 0.2249620025396979 2213 23 0.34511990715837726 2213 24 0.27513393105106587 2213 25 0.3987403140852166 2214 1 0.16285229022448533 2214 2 -0.04973025101093498 2214 3 0.06985079130733851 2214 4 0.1070773461270349 2214 5 0.034981904861353996 2214 6 -0.02874992891923256 2214 7 -0.061406033063235554 2214 8 0.050538866235100954 2214 9 -0.18826419432736685 2214 10 -0.3875054365324786 2214 11 -0.14357820843346758 2214 12 -0.045528578107102646 2214 13 -0.0227577475166143 2214 14 -0.22551764332030189 2214 15 -0.2006075508519345 2214 16 -0.1615143151757863 2214 17 -0.04175339597099992 2214 18 -0.1814246820221186 2214 19 -0.06169508016519387 2214 20 -0.11483187655284866 2214 21 -0.2961766022523449 2214 22 -0.35812210326847804 2214 23 -0.05634649018597315 2214 24 -0.6113735953592254 2214 25 -0.31670318042394996 2215 1 0.5721899666631387 2215 2 0.4295622683941005 2215 3 0.5130433899116191 2215 4 0.4698931179157029 2215 5 0.556605011944356 2215 6 0.33826572853853776 2215 7 0.165204178130665 2215 8 0.2418362526600859 2215 9 0.12488128868397297 2215 10 0.2586869405097829 2215 11 0.0013551821877229031 2215 12 0.22748480007723515 2215 13 0.1104475450003477 2215 14 0.279886144288632 2215 15 0.362342382251029 2215 16 0.2247111370372039 2215 17 0.2516926524519292 2215 18 0.3544224622147567 2215 19 0.23856063423768603 2215 20 0.1854544403396048 2215 21 0.2763188593984509 2215 22 0.06940569297280064 2215 23 0.37605322239544137 2215 24 0.44489581426979186 2215 25 0.40383366068914484 2216 1 0.5296234500990948 2216 2 0.30634537521202887 2216 3 -1.0953521458117805 2216 4 -0.8852782747711387 2216 5 -0.038192791996429776 2216 6 0.11162438530728025 2216 7 -0.059514081631959925 2216 8 0.037475448164492775 2216 9 0.01803272895435981 2216 10 0.14590234968407856 2216 11 0.25306128057471966 2216 12 0.06994171790201165 2216 13 -0.061550998221377995 2216 14 -0.10149704626000755 2216 15 0.13866905456781353 2216 16 0.09246426077145742 2216 17 0.026120067112977097 2216 18 0.26660732329325043 2216 19 -0.12913973233946052 2216 20 -0.1412791428243949 2216 21 0.06281234644231404 2216 22 0.02200934376807595 2216 23 -0.10303622310981808 2216 24 -0.16027983302649318 2216 25 -0.25723393994938704 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12246.0 3 10.0 4 164.0 5 1363.0 6 1855.0 7 1314.0 8 990.0 9 905.0 10 798.0 11 668.0 12 660.0 13 662.0 14 613.0 15 594.0 16 627.0 17 670.0 18 817.0 19 882.0 20 1147.0 21 1502.0 22 2049.0 23 2313.0 24 2882.0 25 3331.0 26 4060.0 27 4420.0 28 5048.0 29 6339.0 30 8075.0 31 10689.0 32 13764.0 33 18126.0 34 24825.0 35 34911.0 36 52306.0 37 92118.0 38 211316.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.825635158968495 19.605891605891607 10.19838886505553 28.37008437008437 2 14.48748698745839 21.333704484703354 39.5619396980709 24.61686882976736 3 18.27475661023923 25.19443560814582 29.809078804756744 26.721728976858213 4 12.033123896552578 17.526220862798276 38.108479237571395 32.33217600307775 5 12.494167077748806 38.92411391959043 35.68605641126245 12.895662591398322 6 31.207003302146397 38.68212254632177 17.74330398092093 12.367570170610897 7 27.76334287053158 32.36977152632 22.001782986197096 17.865102616951322 8 25.057807541836663 38.095074835529445 20.14711876545185 16.699998857182038 9 26.299729720978338 15.6041850392276 19.952648791553734 38.14343644824033 10 14.23428982824231 30.105972474582988 34.5640920154959 21.0956456816788 11 34.07873259904427 23.116582832263262 24.439141175103753 18.365543393588716 12 22.621015663091175 25.63631307898586 31.99220654558752 19.750464712335447 13 28.646577304429638 22.574059049164465 25.93904626952656 22.84031737687933 14 20.56168925777865 22.746967483654217 27.75897565797368 28.932367600593455 15 22.744336199754304 31.543962898418233 23.28790865544858 22.42379224637888 16 21.597207144408003 29.148430447154006 27.799235888118172 21.455126520319816 17 21.152480007008737 29.472878286059277 27.846013495626202 21.52862821130578 18 21.085450258449605 28.58163160360649 29.7053255474675 20.627592590476407 19 22.710019254978963 27.720122881428562 27.90848266575756 21.661375197834907 20 22.76432519321822 28.179958632826335 28.467548099814493 20.588168074140945 21 22.26112265637054 27.3679157769621 27.772116261926488 22.59884530474088 22 22.428527079813886 28.4689911038569 27.823349375974892 21.279132440354324 23 21.79477656711557 28.315973313373593 28.078665744861986 21.810584374648847 24 22.33596605333877 27.609659104976775 28.661731348286573 21.392643493397884 25 22.133528370921876 27.766572004868323 28.37454073790491 21.725358886304893 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 213.0 1 213.0 2 204.0 3 195.0 4 195.0 5 195.0 6 365.0 7 535.0 8 535.0 9 535.0 10 837.5 11 1140.0 12 1140.0 13 1140.0 14 1845.5 15 2551.0 16 2551.0 17 2551.0 18 4498.5 19 6446.0 20 6446.0 21 6446.0 22 10110.0 23 13774.0 24 13774.0 25 13774.0 26 19803.5 27 25833.0 28 25833.0 29 25833.0 30 33990.5 31 42148.0 32 42148.0 33 42148.0 34 47838.5 35 53529.0 36 53529.0 37 53529.0 38 57303.5 39 61078.0 40 61078.0 41 61078.0 42 63772.5 43 66467.0 44 66467.0 45 66467.0 46 67738.5 47 69010.0 48 69010.0 49 69010.0 50 65260.0 51 61510.0 52 61510.0 53 61510.0 54 54196.5 55 46883.0 56 46883.0 57 46883.0 58 40154.0 59 33425.0 60 33425.0 61 33425.0 62 26962.0 63 20499.0 64 20499.0 65 20499.0 66 15910.0 67 11321.0 68 11321.0 69 11321.0 70 8304.5 71 5288.0 72 5288.0 73 5288.0 74 3704.5 75 2121.0 76 2121.0 77 2121.0 78 1461.0 79 801.0 80 801.0 81 801.0 82 502.0 83 203.0 84 203.0 85 203.0 86 134.0 87 65.0 88 65.0 89 65.0 90 43.0 91 21.0 92 21.0 93 21.0 94 12.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1112255955997326 2 0.10760695464700157 3 0.014855473384895793 4 0.0 5 0.004190005313688557 6 0.3357717894560421 7 0.01847411433762682 8 0.007808646266419584 9 0.0087609202013488 10 0.0032377313787593396 11 0.003047276591773496 12 5.713643609575305E-4 13 9.522739349292175E-4 14 0.0 15 0.002666367017801809 16 9.522739349292175E-4 17 0.0 18 9.522739349292175E-4 19 0.0 20 9.522739349292175E-4 21 0.013712744662980732 22 0.0 23 3.80909573971687E-4 24 0.0 25 0.006094553183546992 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 525059.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.76404611057396 #Duplication Level Percentage of deduplicated Percentage of total 1 90.10834005607714 74.57730811348448 2 7.603002450947943 12.585104908581249 3 1.2715599443242955 3.157183375932446 4 0.40849567007847876 1.352350178973801 5 0.19338908957299397 0.8002831763350596 6 0.10992921555127318 0.5458911998870879 7 0.05661920670370095 0.32802242440584567 8 0.04084021532153588 0.2704081171229892 9 0.032004715952062945 0.23839558081311238 >10 0.14885359632627373 2.254470820572732 >50 0.012215304973593641 0.6926219679268036 >100 0.013137194495784914 2.04753379656787 >500 0.001382862578499981 0.8913848920576971 >1k 2.3047709641666356E-4 0.25904144733883 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1358 0.25863760072677544 No Hit CTGTAGGACGTGGAATATGGCAAGA 863 0.16436248116878294 No Hit GGTATCAACGCAGAGTACTTTTTTT 861 0.16398157159481125 No Hit GTCCTACAGTGGACATTTCTAAATT 848 0.16150565936399527 No Hit TATCAACGCAGAGTACTTTTTTTTT 758 0.14436472853526935 No Hit GTCCTAAAGTGTGTATTTCTCATTT 710 0.13522289875994886 No Hit CTTTAGGACGTGAAATATGGCGAGG 633 0.12055788016203893 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.904547869858435E-4 0.0 7 0.0 0.0 0.0 1.904547869858435E-4 0.0 8 0.0 0.0 0.0 1.904547869858435E-4 0.0 9 0.0 0.0 0.0 1.904547869858435E-4 0.0 10 0.0 0.0 0.0 1.904547869858435E-4 0.0 11 0.0 0.0 0.0 1.904547869858435E-4 0.0 12 0.0 0.0 0.0 1.904547869858435E-4 0.0 13 0.0 0.0 0.0 1.904547869858435E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCTGCG 35 1.0021188E-4 18.975811 9 TCCAACG 45 6.8222353E-4 14.757558 18 TAGGACC 155 0.0 14.748539 4 CACTGTT 125 1.4260877E-9 12.145676 7 ACTGTTC 135 3.765308E-10 11.947733 8 TTAGACA 65 7.832509E-4 11.723198 4 GATATAC 200 0.0 11.439961 1 GTAGGAC 370 0.0 11.328227 3 ACATATC 60 0.0057486338 11.113677 3 TACCAAT 60 0.0057527283 11.112615 6 GTAGACT 60 0.0057527283 11.112615 4 AAGCTTC 60 0.0057527283 11.112615 6 TGTAGGA 395 0.0 11.099948 2 TAGACAG 70 0.00145613 10.885826 5 TTACACA 70 0.00145613 10.885826 4 GTCCTAC 405 0.0 10.827947 1 AAATTTT 115 8.928564E-7 10.724437 16 CTAGGAC 80 3.667814E-4 10.71676 3 TAGGACT 80 3.671058E-4 10.715735 4 GGACAGT 80 3.671058E-4 10.715735 6 >>END_MODULE