FastQCFastQC Report
Thu 26 May 2016
SRR1926770_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926770_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786359
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17590.22368918013273836No Hit
CTGTAGGACGTGGAATATGGCAAGA13790.175365195794796No Hit
GTCCTACAGTGTGCATTTCTCATTT12400.15768879099749605No Hit
GTCCTAAAGTGTGTATTTCTCATTT11340.14420894273480686No Hit
GTATCAACGCAGAGTACTTTTTTTT8850.11254401615547098No Hit
CTTTAGGACGTGAAATATGGCGAGG8280.10529541850477961No Hit
CTGTAGGACCTGGAATATGGCGAGA7910.10059018845082209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3650.014.094474
GTACAAG1153.5288394E-1013.2708221
AGGACCT5400.013.0485945
TCCAACG905.5320925E-712.64020318
GGACCTG5600.012.5825746
TGTAGGA8750.012.4224052
AAGGTGT1001.3962563E-712.3785316
CCAACGA854.02942E-612.26843319
GCCTAGG701.0492893E-412.263661
GTAGGAC9150.012.1919643
CTGTAGG8800.012.1397841
TAGGGCT550.003002049212.1250394
GTCCTAC9500.012.0485081
CTAGGCT802.7807095E-511.9085224
CATTTCT11750.011.6182314
TTAGGCA752.0177779E-411.432184
TCCTACA10450.011.4051912
CCTACAG10400.011.2766653
ATTTCTA6000.011.21818115
CCTAACC600.00571384111.1238643
Produced by FastQC (version 0.11.5)