##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926770_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 786359 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.881047969184557 31.0 28.0 31.0 18.0 33.0 2 28.472335154808427 31.0 28.0 31.0 22.0 34.0 3 28.40652424655914 31.0 28.0 33.0 16.0 34.0 4 32.18552594934375 35.0 33.0 37.0 22.0 37.0 5 32.00465309101823 35.0 32.0 37.0 19.0 37.0 6 31.69118430640458 35.0 32.0 37.0 17.0 37.0 7 31.78602139735159 35.0 32.0 37.0 17.0 37.0 8 31.622159853196823 35.0 32.0 37.0 17.0 37.0 9 33.66310425645284 38.0 34.0 39.0 21.0 39.0 10 33.74273709590658 38.0 34.0 39.0 22.0 39.0 11 33.92408556397269 38.0 34.0 39.0 25.0 39.0 12 33.76107477627903 38.0 34.0 39.0 22.0 39.0 13 33.88820754896936 38.0 35.0 39.0 23.0 39.0 14 34.94492973311172 39.0 35.0 41.0 24.0 41.0 15 34.51198371227391 39.0 34.0 41.0 18.0 41.0 16 34.61017041834582 39.0 34.0 41.0 19.0 41.0 17 34.8570334923362 39.0 35.0 41.0 24.0 41.0 18 34.90089132317428 39.0 35.0 41.0 24.0 41.0 19 34.285362029302135 39.0 34.0 41.0 18.0 41.0 20 34.551828109044344 39.0 34.0 41.0 18.0 41.0 21 34.54216712722815 39.0 34.0 41.0 18.0 41.0 22 34.579063760953964 39.0 35.0 41.0 18.0 41.0 23 34.5917297315857 39.0 35.0 41.0 18.0 41.0 24 33.29910130105969 39.0 33.0 41.0 2.0 41.0 25 33.888830674030565 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.041601808757956604 1101 2 -0.1506434968771444 1101 3 -0.9031906553381397 1101 4 -0.08187171695472273 1101 5 -0.1166863636287232 1101 6 -1.6482305224308007 1101 7 -0.14784378625015648 1101 8 0.41487657689543767 1101 9 0.24950286711379022 1101 10 -9.309242743919135 1101 11 -3.896958368061835 1101 12 -1.5911728045507019 1101 13 -0.667606881083934 1101 14 -0.4096455196127522 1101 15 -1.693161091268422 1101 16 0.001012623488733766 1101 17 -0.06775522309360582 1101 18 0.11646812126221562 1101 19 -0.33490512223778524 1101 20 0.18106145272687257 1101 21 0.09360754427426343 1101 22 0.18570020245172003 1101 23 0.12567563077674748 1101 24 0.1342996940240937 1101 25 0.19341540812168034 1102 1 -0.400453880692929 1102 2 -0.40854082407498993 1102 3 -0.4618944731093144 1102 4 -0.35017250079955176 1102 5 -0.24871711353511827 1102 6 -1.003746120822047 1102 7 -0.06078447250690644 1102 8 0.50877792597813 1102 9 0.13080593365002358 1102 10 0.08632110501259405 1102 11 -0.20034345447800916 1102 12 -0.15647257386601154 1102 13 -0.16671312889484113 1102 14 -0.4532144291143041 1102 15 -0.009886032993364324 1102 16 0.08348994754636863 1102 17 -0.21641852007296336 1102 18 -0.38541751469077923 1102 19 0.14072201401111073 1102 20 -0.22697718393870758 1102 21 -0.11886241236668127 1102 22 -0.19233281806835123 1102 23 -0.45172451657158064 1102 24 -3.716209684335027 1102 25 -2.0046729888009125 1103 1 -0.4981104376191503 1103 2 -0.6421877950815116 1103 3 0.027223150839770227 1103 4 -0.3886340392610883 1103 5 -0.5697079204809832 1103 6 0.3025220492101681 1103 7 0.1621078037561574 1103 8 0.4961472981789612 1103 9 0.05924547164011784 1103 10 -0.056524813114229744 1103 11 -0.3844304349133054 1103 12 -0.33346833091795247 1103 13 -0.34417050298143437 1103 14 -0.3452451512012047 1103 15 0.027524590091417167 1103 16 -0.10564535535298347 1103 17 -0.4551531000996789 1103 18 -0.644129308514934 1103 19 -2.6521592620162338 1103 20 -1.2098384238224185 1103 21 -0.7615020760737607 1103 22 -0.5595442583135011 1103 23 -0.5746914372913139 1103 24 0.850253347015645 1103 25 0.08367265432001147 1104 1 -0.3692171080878701 1104 2 0.01453237297518939 1104 3 -1.070566278712068 1104 4 -0.21242576330856622 1104 5 -0.3126035680865371 1104 6 -1.31601473456125 1104 7 -0.053393525857647006 1104 8 0.48865887197160873 1104 9 0.45574080270253603 1104 10 0.46149920909095243 1104 11 0.4389987676647209 1104 12 0.31977251523073846 1104 13 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0.19870211816795091 2215 3 0.5554430042281631 2215 4 0.5376907470636993 2215 5 0.8099956551776479 2215 6 1.3377107556348307 2215 7 0.7107591690367343 2215 8 -0.45164424444404005 2215 9 0.09064276348684785 2215 10 0.08796316665465298 2215 11 -0.0399251690597211 2215 12 -0.1745080569014945 2215 13 0.09428143209971296 2215 14 0.11023933933946495 2215 15 0.31276483465750005 2215 16 0.4250348840913034 2215 17 -0.022485601140047606 2215 18 -0.7611945154677784 2215 19 0.4570234903125865 2215 20 -3.6270406390021606 2215 21 -1.949006157510425 2215 22 -0.8538660996016318 2215 23 -0.4755525403996046 2215 24 -0.5203731134984579 2215 25 -0.29866288779081174 2216 1 -0.2225115400147395 2216 2 -1.2582736384304454 2216 3 -0.6336189750224968 2216 4 -0.8378357809156611 2216 5 -0.5825012539230343 2216 6 -2.136299887809777 2216 7 -10.414228198592141 2216 8 -11.892561300832796 2216 9 -6.636478668540242 2216 10 -3.827255884036095 2216 11 -2.841704451971083 2216 12 -2.6173678107046463 2216 13 -2.265208972673708 2216 14 -2.2125989125553858 2216 15 -3.257989344775922 2216 16 -2.2364896012067703 2216 17 -2.6176422962967365 2216 18 -2.348357876216049 2216 19 -1.672445950100247 2216 20 -2.518753803545515 2216 21 -5.187522525838116 2216 22 -3.6644267618604083 2216 23 -3.66428554045336 2216 24 -3.9933075864329304 2216 25 -3.521446309819517 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39087.0 3 7.0 4 341.0 5 1905.0 6 2356.0 7 1977.0 8 1550.0 9 1529.0 10 1389.0 11 1332.0 12 1249.0 13 1309.0 14 1328.0 15 1327.0 16 1407.0 17 1590.0 18 1865.0 19 2208.0 20 2959.0 21 3819.0 22 4861.0 23 5734.0 24 6799.0 25 7695.0 26 8967.0 27 9662.0 28 11022.0 29 13198.0 30 15842.0 31 18916.0 32 24870.0 33 32355.0 34 44183.0 35 62301.0 36 91723.0 37 150676.0 38 207021.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32981559936579 18.576949155998758 12.301362739550035 25.791872505085422 2 13.972293285215656 20.268637216237934 42.989722359820554 22.769347138725855 3 19.234736498945587 24.184359050112572 29.61941849448344 26.961485956458397 4 11.01454955923731 16.85231681973843 39.90941680866687 32.22371681235739 5 12.462365249467359 38.18564568957294 37.007027697395614 12.344961363564092 6 31.1998290483053 38.29432437829677 18.774159328505814 11.731687244892118 7 27.596201440023677 31.48469911057361 22.781536875730346 18.137562573672366 8 23.6387477295455 38.85678789363048 21.589853874568867 15.914610502255147 9 25.752723184077574 15.670836873515032 20.530849185715812 38.045590756691574 10 13.0406684442997 30.760472429725823 36.1933633902583 20.005495735716174 11 34.384853229410844 23.04881414655055 24.704570054195543 17.86176256984306 12 22.098084256100694 25.58806545733902 33.309675893068366 19.004174393491915 13 28.822318122790886 22.597175639220534 26.098182340813324 22.482323897175256 14 19.218333846337785 23.26225041909496 28.87379836126876 28.6456173732985 15 22.272800694955848 32.583559410203286 23.283691813028387 21.85994808181248 16 20.467079790995722 29.595372193476503 29.510534161617088 20.42701385391069 17 20.410322941707687 29.69779066661579 28.83580722707674 21.05607916459979 18 19.886980786988165 29.360829672440154 30.96085409252669 19.791335448044993 19 22.718287787281024 27.8848942459843 27.969354994632162 21.42746297210251 20 22.02127632512789 28.951090398943812 29.264359102853376 19.76327417307492 21 21.82484416276517 28.01369052842845 27.98240225275585 22.17906305605053 22 21.195567149694554 29.763387139239477 28.640674822982344 20.40037088808363 23 20.990997407843626 28.98648975220613 28.682375626098615 21.34013721385163 24 21.97026064858801 28.141474960921155 29.45813634924087 20.430128041249965 25 21.39128709446019 28.6579347676779 29.095589619401807 20.855188518460103 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 170.0 1 170.0 2 272.5 3 375.0 4 375.0 5 375.0 6 591.0 7 807.0 8 807.0 9 807.0 10 1248.0 11 1689.0 12 1689.0 13 1689.0 14 3012.0 15 4335.0 16 4335.0 17 4335.0 18 7351.0 19 10367.0 20 10367.0 21 10367.0 22 16109.5 23 21852.0 24 21852.0 25 21852.0 26 31677.0 27 41502.0 28 41502.0 29 41502.0 30 54646.0 31 67790.0 32 67790.0 33 67790.0 34 77242.0 35 86694.0 36 86694.0 37 86694.0 38 93397.0 39 100100.0 40 100100.0 41 100100.0 42 103327.0 43 106554.0 44 106554.0 45 106554.0 46 105887.5 47 105221.0 48 105221.0 49 105221.0 50 98309.5 51 91398.0 52 91398.0 53 91398.0 54 77981.0 55 64564.0 56 64564.0 57 64564.0 58 53082.0 59 41600.0 60 41600.0 61 41600.0 62 32328.5 63 23057.0 64 23057.0 65 23057.0 66 17096.0 67 11135.0 68 11135.0 69 11135.0 70 7895.0 71 4655.0 72 4655.0 73 4655.0 74 3156.5 75 1658.0 76 1658.0 77 1658.0 78 1105.0 79 552.0 80 552.0 81 552.0 82 369.5 83 187.0 84 187.0 85 187.0 86 122.5 87 58.0 88 58.0 89 58.0 90 41.0 91 24.0 92 24.0 93 24.0 94 13.0 95 2.0 96 2.0 97 2.0 98 7.5 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2241978536520851 2 0.1350528193865652 3 0.13823202888248243 4 0.07146862946821998 5 0.02339898188995103 6 0.021872961331910745 7 0.3157590871345022 8 0.3020249021121396 9 0.018693751835993484 10 0.037514672051823655 11 0.01678622613844313 12 0.017422068037626578 13 0.01627755261909637 14 0.01793074155697334 15 0.015514542340076223 16 0.019711098874687007 17 0.018948088595666865 18 0.015387373960239535 19 0.024797834068154622 20 0.016913394518279817 21 0.015514542340076223 22 0.01754923641746327 23 0.01793074155697334 24 0.01627755261909637 25 0.015641710719912917 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 786359.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.88159263832786 #Duplication Level Percentage of deduplicated Percentage of total 1 94.89116232540222 83.39186472458441 2 3.9903483570572225 7.013563375998473 3 0.5304829134582077 1.3985904990638263 4 0.181144857267166 0.636771942195245 5 0.09771792779493171 0.42938035619678616 6 0.06073475743617033 0.32024803271959046 7 0.03819551039341865 0.23496775995052047 8 0.03107860426445565 0.2184989791789352 9 0.020578938102419408 0.16276588697215674 >10 0.13292080104677037 2.1599653831025463 >50 0.01390470183765805 0.8202255823807028 >100 0.010282120062486551 1.9507774439871197 >500 8.689115261893847E-4 0.5608739599135092 >1k 5.792743507929231E-4 0.7015060737561295 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1759 0.22368918013273836 No Hit CTGTAGGACGTGGAATATGGCAAGA 1379 0.175365195794796 No Hit GTCCTACAGTGTGCATTTCTCATTT 1240 0.15768879099749605 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1134 0.14420894273480686 No Hit GTATCAACGCAGAGTACTTTTTTTT 885 0.11254401615547098 No Hit CTTTAGGACGTGAAATATGGCGAGG 828 0.10529541850477961 No Hit CTGTAGGACCTGGAATATGGCGAGA 791 0.10059018845082209 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.2716837983669038E-4 0.0 10 0.0 0.0 0.0 1.2716837983669038E-4 0.0 11 0.0 0.0 0.0 1.2716837983669038E-4 0.0 12 0.0 0.0 0.0 1.2716837983669038E-4 0.0 13 0.0 0.0 0.0 2.5433675967338076E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 365 0.0 14.09447 4 GTACAAG 115 3.5288394E-10 13.270822 1 AGGACCT 540 0.0 13.048594 5 TCCAACG 90 5.5320925E-7 12.640203 18 GGACCTG 560 0.0 12.582574 6 TGTAGGA 875 0.0 12.422405 2 AAGGTGT 100 1.3962563E-7 12.378531 6 CCAACGA 85 4.02942E-6 12.268433 19 GCCTAGG 70 1.0492893E-4 12.26366 1 GTAGGAC 915 0.0 12.191964 3 CTGTAGG 880 0.0 12.139784 1 TAGGGCT 55 0.0030020492 12.125039 4 GTCCTAC 950 0.0 12.048508 1 CTAGGCT 80 2.7807095E-5 11.908522 4 CATTTCT 1175 0.0 11.61823 14 TTAGGCA 75 2.0177779E-4 11.43218 4 TCCTACA 1045 0.0 11.405191 2 CCTACAG 1040 0.0 11.276665 3 ATTTCTA 600 0.0 11.218181 15 CCTAACC 60 0.005713841 11.123864 3 >>END_MODULE