FastQCFastQC Report
Thu 26 May 2016
SRR1926770_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926770_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786359
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17430.2216544860553513No Hit
CTGTAGGACGTGGAATATGGCAAGA15500.19711098874687005No Hit
GTATCAACGCAGAGTACTTTTTTTT15090.19189708517356577No Hit
GTCCTACAGTGTGCATTTCTCATTT12470.15857896965635288No Hit
GTCCTAAAGTGTGTATTTCTCATTT11440.14548062653317378No Hit
CTTTAGGACGTGAAATATGGCGAGG10840.13785052374297235No Hit
CACACACACACACACACACACACAC10110.12856723201489395No Hit
GGTATCAACGCAGAGTACTTTTTTT10080.12818572687538388No Hit
CTGTAGGACCTGGAATATGGCGAGA9570.12170013950371268No Hit
TATCAACGCAGAGTACTTTTTTTTT8630.10974631179906377No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTG350.00213064616.3291653
AAAGGCG508.514174E-515.239585
TCCAACG902.3246685E-914.75784618
CCAACGA456.8265677E-414.75784619
TAGGACC4200.014.7406674
CACCGTG400.00531226414.2353077
GGCGAGG3000.013.91454119
CTTACTC551.914435E-413.8550493
GTATTAG903.475543E-813.7676241
GTGCTAC655.2795564E-513.1973671
AGGACCT6400.013.0965165
TAATACC603.9992906E-412.6996514
AAGACTG905.2260657E-712.6996515
TAGGACA2100.012.2460934
TCCTAAG1251.3351382E-912.1994532
GTAGGAC9350.012.1231683
CTGTAGG9200.012.0178921
TGTAGGA9950.011.781822
GTCTAGA657.796538E-411.7309931
TGGATTG657.8426383E-411.7227555
Produced by FastQC (version 0.11.5)