FastQCFastQC Report
Thu 26 May 2016
SRR1926769_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926769_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824814
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17600.21338144114915603No Hit
GTCCTACAGTGTGCATTTCTCATTT12860.155913939385122No Hit
GTCCTAAAGTGTGTATTTCTCATTT12830.15555022101952684No Hit
CTGTAGGACGTGGAATATGGCAAGA12490.15142807954278176No Hit
GTATCAACGCAGAGTACTTTTTTTT8890.10778187567136348No Hit
CTTTAGGACGTGAAATATGGCGAGG8770.10632700220898288No Hit
CTGTAGGACCTGGAATATGGCGAGA8590.10414469201541197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCA307.822201E-418.9623412
GGTCTAA456.550726E-414.8384721
ATCCCGT551.989582E-413.79246610
TAGGACC3400.013.7247984
TCCAACG903.7232894E-813.69585518
TATGTCG701.1094425E-412.19081616
GGATAGG1052.5730697E-711.8102131
TGGCGAG7300.011.55993618
ATGTCGA752.1088989E-411.37809417
TGTCGAG752.1088989E-411.37809418
GTAGGAC9050.011.167323
TAATTCT1451.1641532E-911.1652824
CCAACGA855.4207558E-511.15499419
CTAGTAC600.005715931811.1234253
TAGGACT1552.9831426E-1011.0593514
GGCGAGA4900.011.02978619
GGACCTG6100.010.9237546
GATATAC2800.010.9017341
AGGACCT6550.010.9005925
TGTAGGA10150.010.8970792
Produced by FastQC (version 0.11.5)