##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1926769_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	824814
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.949038207401912	31.0	28.0	31.0	18.0	33.0
2	28.530695405267128	31.0	29.0	31.0	22.0	34.0
3	28.440648437102183	31.0	28.0	33.0	16.0	34.0
4	32.2081232859772	35.0	33.0	37.0	22.0	37.0
5	32.049390529258716	35.0	32.0	37.0	20.0	37.0
6	31.713049245041912	35.0	32.0	37.0	17.0	37.0
7	31.7846678160167	35.0	32.0	37.0	17.0	37.0
8	31.60295169577626	35.0	32.0	37.0	17.0	37.0
9	33.62050474409988	38.0	34.0	39.0	19.0	39.0
10	33.68509021427861	38.0	34.0	39.0	21.0	39.0
11	33.86288666293249	38.0	34.0	39.0	23.0	39.0
12	33.68067709810939	38.0	34.0	39.0	22.0	39.0
13	33.813930170923385	38.0	34.0	39.0	23.0	39.0
14	34.89124457150339	39.0	35.0	41.0	24.0	41.0
15	34.453508306115076	39.0	34.0	41.0	17.0	41.0
16	34.56181999820566	39.0	34.0	41.0	18.0	41.0
17	34.80960677195101	39.0	35.0	41.0	23.0	41.0
18	34.86168760472058	39.0	35.0	41.0	23.0	41.0
19	34.22252289607111	39.0	34.0	41.0	16.0	41.0
20	34.47165179058551	39.0	34.0	41.0	18.0	41.0
21	34.4612821799824	39.0	34.0	41.0	18.0	41.0
22	34.50828429197371	39.0	34.0	41.0	18.0	41.0
23	34.51799193515144	39.0	35.0	41.0	18.0	41.0
24	33.22159783902795	39.0	33.0	41.0	2.0	41.0
25	33.810240854301696	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.2176771274744631
1101	2	-0.2690074945094736
1101	3	-0.9944908760283866
1101	4	-0.2820478737923757
1101	5	-0.20189174249107822
1101	6	-2.2880418256940906
1101	7	-0.42175463346458386
1101	8	0.131486893835131
1101	9	-0.19778844853179578
1101	10	-8.875521120931811
1101	11	-3.524852646695482
1101	12	-1.1353079537126902
1101	13	-0.46418440451166276
1101	14	-0.2568092634949224
1101	15	-1.8767095114394365
1101	16	-0.16205168392440328
1101	17	-0.08746207086502267
1101	18	-0.01846088149728331
1101	19	-0.6050425763760288
1101	20	-0.11327872233464831
1101	21	-0.2689594628877785
1101	22	0.1581075161927501
1101	23	-0.0210852862507096
1101	24	-0.0635443784820211
1101	25	0.020654612816244367
1102	1	-0.6832362153353344
1102	2	-0.5718641723903524
1102	3	-0.6248610011430777
1102	4	-0.6294326428534944
1102	5	-0.4038457651570653
1102	6	-1.0426989036950616
1102	7	-0.15195977217381795
1102	8	0.24878577456511053
1102	9	0.02783607271300781
1102	10	0.14042756492901987
1102	11	-0.3855499667887514
1102	12	-0.018058176079570387
1102	13	-0.22610453201136238
1102	14	-0.5708881034272437
1102	15	0.10497924226784505
1102	16	0.025179827356680562
1102	17	-0.07658503343724021
1102	18	-0.26442811429927815
1102	19	-0.021766529989037053
1102	20	-0.26639340191589866
1102	21	-0.368518096194272
1102	22	-0.4026091645501353
1102	23	-0.4007111487115296
1102	24	-4.061169191539513
1102	25	-2.0321101721262202
1103	1	-0.34396639372019777
1103	2	-0.43224498841679804
1103	3	0.07150330861750831
1103	4	-0.049802071435976814
1103	5	-0.4945348713532738
1103	6	0.49114419280523336
1103	7	0.4225809987450795
1103	8	0.5795702920636288
1103	9	0.027585935897846525
1103	10	0.22378669506097282
1103	11	6.847693376386133E-4
1103	12	-0.3256725618485845
1103	13	-0.24703863408929294
1103	14	-0.19438244563652063
1103	15	0.20529930683355246
1103	16	0.05570895654889085
1103	17	-0.05790769058120304
1103	18	-0.26792864774022007
1103	19	-1.710640555008247
1103	20	-0.7592914511251365
1103	21	-0.612919730237202
1103	22	-0.6378676761117106
1103	23	-0.5064250474738401
1103	24	0.8821257598427366
1103	25	0.28385961245208335
1104	1	-0.34928739354621285
1104	2	-0.12710045771697054
1104	3	-1.2605955693260604
1104	4	-0.3057106112713299
1104	5	-0.38522136110895033
1104	6	-1.6758048520797857
1104	7	0.011276756581942493
1104	8	0.3877153502376842
1104	9	0.3041032527938654
1104	10	0.17466047959189268
1104	11	0.1285924364931148
1104	12	0.19583720194790288
1104	13	-0.10274819739034768
1104	14	0.10629926647301602
1104	15	0.3330695821419596
1104	16	-0.11831266160947962
1104	17	-0.07471989594818496
1104	18	-0.04132530559723335
1104	19	-1.3860243546453646
1104	20	-0.40207471997405264
1104	21	-0.17244975346263658
1104	22	-0.023661221042296177
1104	23	0.10125080718629675
1104	24	1.4092078331754578
1104	25	0.6954158547367371
1105	1	-0.3371125461119533
1105	2	-0.21623737152999922
1105	3	0.471006320258919
1105	4	-0.03329206414321817
1105	5	-0.176736475761345
1105	6	-0.2959840788097061
1105	7	0.016706272223732554
1105	8	0.30139051192696087
1105	9	-0.6121771843247217
1105	10	-0.02778259970174446
1105	11	-0.23971849349897667
1105	12	-0.14419483178889436
1105	13	-0.1373770723574026
1105	14	-0.5161081296707692
1105	15	0.15863372057636127
1105	16	-0.544251878607632
1105	17	-0.43363764223288115
1105	18	-0.4205297335517386
1105	19	-0.9946612988701702
1105	20	-0.26418975758328145
1105	21	-0.1763505705256705
1105	22	-0.19733881567360356
1105	23	-0.16242942313221675
1105	24	-4.859397077312547
1105	25	-2.1939096120840134
1106	1	0.04505064798962266
1106	2	-0.8004676470311338
1106	3	-0.16496352931811842
1106	4	-0.5294804713714178
1106	5	-0.10718971956503509
1106	6	0.2968370923169843
1106	7	-2.439954140534244
1106	8	-0.3031452176408642
1106	9	-0.6081713912303499
1106	10	0.08618665739973608
1106	11	-0.25685420331761577
1106	12	0.03735284603606459
1106	13	-0.225195116529747
1106	14	-0.4634699676616165
1106	15	0.1988726857190457
1106	16	0.14460628932626918
1106	17	-0.2725007474631198
1106	18	-0.189158152629453
1106	19	0.10481534771815859
1106	20	0.11383927371917935
1106	21	-0.4359536119002101
1106	22	-0.4966541772482671
1106	23	-0.41254298094795416
1106	24	-3.7263533986976256
1106	25	-1.5842917588204628
1107	1	0.22291532467853514
1107	2	0.15557020113549314
1107	3	0.3468109077117809
1107	4	0.1623549410676972
1107	5	0.2653253022894191
1107	6	-0.02577544414821986
1107	7	-2.18458952140033
1107	8	-1.9609330666297033
1107	9	-0.473274316010901
1107	10	-0.11929323617285092
1107	11	-0.4912477545267606
1107	12	-0.24456666247714054
1107	13	-0.5644209853635473
1107	14	-0.2629792701937106
1107	15	-1.7631054960034618
1107	16	-0.8960406946273949
1107	17	-0.7182836105447166
1107	18	-0.641042503261076
1107	19	0.23829624110328496
1107	20	0.26283962562688146
1107	21	0.04515881109941944
1107	22	-0.2406620441942664
1107	23	-0.4202507462682732
1107	24	-4.704513414747247
1107	25	-2.662329100503108
1108	1	0.7915837487561355
1108	2	0.4369873464879319
1108	3	0.9730147395525997
1108	4	0.9899895323091101
1108	5	1.2432095379280454
1108	6	-2.6770599488569466
1108	7	-0.7404710149641609
1108	8	0.5533973244852035
1108	9	0.437332761367621
1108	10	0.8056278569324675
1108	11	0.6819113789262303
1108	12	0.8016389032283016
1108	13	0.754965226559662
1108	14	0.716347926115084
1108	15	-0.25412054530622186
1108	16	0.6408697512647876
1108	17	0.4533413738023384
1108	18	0.7076973945693439
1108	19	0.6021573649686474
1108	20	-0.5392403697494785
1108	21	0.3831211348633232
1108	22	0.5824388121422004
1108	23	0.8682174485538923
1108	24	0.026897469427382248
1108	25	0.6828859147645332
1109	1	0.32473687500369053
1109	2	0.27842197367563415
1109	3	-1.1182496864106284
1109	4	0.0016948662966385086
1109	5	0.4577502384195995
1109	6	0.4912152742940812
1109	7	0.8175750688839436
1109	8	1.1312580611807093
1109	9	0.5915944964144444
1109	10	0.8260934494673862
1109	11	0.45698246117441244
1109	12	0.02745707285659904
1109	13	0.2725683301035673
1109	14	0.37541765528638393
1109	15	-1.0343388051396474
1109	16	0.09608260183816952
1109	17	-0.03174522007041958
1109	18	0.3256293201744924
1109	19	-1.410514017150625
1109	20	0.0047404189968176524
1109	21	-0.054200016714808896
1109	22	0.45943071501322663
1109	23	0.3130298919852237
1109	24	-0.5969916514078619
1109	25	-0.05345791304119274
1110	1	0.24378082257501177
1110	2	0.34318681561858
1110	3	0.6928528579224462
1110	4	0.67709964655878
1110	5	0.5926769924674034
1110	6	0.5660032465377149
1110	7	0.725824876131437
1110	8	0.7881586008877264
1110	9	0.46977491275985983
1110	10	0.39278581646815525
1110	11	0.33747590758921575
1110	12	0.23794280917117305
1110	13	0.39670094382753973
1110	14	0.1911462597582414
1110	15	0.7767637391934272
1110	16	0.5016338740047104
1110	17	-0.1358315731930091
1110	18	0.07196702256462828
1110	19	-1.9139835622547139
1110	20	-0.17413390636941273
1110	21	-0.061910116946116034
1110	22	0.008389851482000665
1110	23	0.015420023056925913
1110	24	-0.6825737639753271
1110	25	-0.4829104959247701
1111	1	0.5153727345810957
1111	2	0.1691634041054897
1111	3	0.9602289080386797
1111	4	0.6793092555399127
1111	5	0.5431359671228435
1111	6	0.8304683997316538
1111	7	0.7385398598135602
1111	8	1.1384127088256406
1111	9	0.41116478760291386
1111	10	0.37838878755847105
1111	11	0.2709412867989016
1111	12	0.3334998275992973
1111	13	0.18060897110514418
1111	14	0.22568367407997414
1111	15	-0.8655672094510294
1111	16	-0.5751841728589895
1111	17	-0.11767768609978191
1111	18	0.1282507542531377
1111	19	0.6238473489141256
1111	20	0.012372081885906994
1111	21	0.2658278256365918
1111	22	0.3753086990457035
1111	23	0.14603272328277228
1111	24	0.7481338163837279
1111	25	0.5042321711424123
1112	1	0.5783125357406753
1112	2	0.7187035842143992
1112	3	-1.3622644739874836
1112	4	0.38056954053617886
1112	5	0.5661690166420001
1112	6	1.4665917958178163
1112	7	1.3188693888775198
1112	8	1.739285519836315
1112	9	0.6660923223522488
1112	10	0.9101050506679726
1112	11	0.7544580138769561
1112	12	0.9466487101306882
1112	13	0.5788058360780184
1112	14	0.9030141158503255
1112	15	1.188457507502008
1112	16	1.2838060988047175
1112	17	0.5812025152184646
1112	18	0.8477077678277993
1112	19	1.5769753858224718
1112	20	1.2540790133397195
1112	21	1.2792246051355889
1112	22	1.109021777344097
1112	23	1.156354375565499
1112	24	2.4177761559582436
1112	25	1.4675221651296866
1113	1	-0.3087295289484473
1113	2	0.19444013524507042
1113	3	-4.6120650437880535
1113	4	-1.0503471680275887
1113	5	0.25020119361535365
1113	6	0.6145488931866758
1113	7	0.8978806173005118
1113	8	1.4358801234897385
1113	9	1.0809574136879263
1113	10	1.0966979784373692
1113	11	0.6085019220385135
1113	12	0.6803038131975612
1113	13	0.15082527633275333
1113	14	-0.14114878007727327
1113	15	0.5569173700795176
1113	16	0.8614933788449406
1113	17	0.7405086992305314
1113	18	0.5941766801790607
1113	19	1.3842185565950516
1113	20	0.8156476407907007
1113	21	0.8298463581102808
1113	22	0.9458409494573843
1113	23	0.9298921667503492
1113	24	2.094243392425483
1113	25	1.4498482921616969
1114	1	0.5443941210173087
1114	2	0.36081914583460417
1114	3	0.37877210915436166
1114	4	0.24913863356347576
1114	5	0.5646124469739746
1114	6	1.0740884327262172
1114	7	1.0878716609757682
1114	8	0.8220325917474725
1114	9	0.3993020741378501
1114	10	0.32831380479004224
1114	11	0.21026473748553798
1114	12	-0.2822882525524051
1114	13	0.22633130868089069
1114	14	0.29960039014557793
1114	15	-1.824532500844036
1114	16	0.04951929950244249
1114	17	0.17784392919734415
1114	18	0.38074008568983686
1114	19	1.0420732533971204
1114	20	0.8894930651624406
1114	21	0.5230353365624225
1114	22	0.6211267615853018
1114	23	0.14159861003574292
1114	24	1.0548808720103295
1114	25	0.5721695607987556
1115	1	-3.4688192837067717
1115	2	-1.1105665963618065
1115	3	-0.27384588378699704
1115	4	0.23368810031274023
1115	5	0.7233365385112052
1115	6	1.176889380502221
1115	7	0.8181630808046485
1115	8	0.9676654512266012
1115	9	0.588942947332761
1115	10	-0.5747388896495309
1115	11	0.0607618940561494
1115	12	-0.05687207973029729
1115	13	0.137353615077771
1115	14	0.1717616499536163
1115	15	0.5546787808131342
1115	16	0.4593107543102164
1115	17	0.30402497388009664
1115	18	0.29432345560737616
1115	19	-1.7770300904839544
1115	20	-0.39479664526299274
1115	21	-0.017902645347916746
1115	22	0.14532353967411638
1115	23	0.07677054905282432
1115	24	-1.2246932776729835
1115	25	-0.5339092643970034
1116	1	0.6488230270392492
1116	2	-0.3282533750119292
1116	3	0.1183683683474861
1116	4	-0.13898033104647567
1116	5	0.8647302868815743
1116	6	1.201126589620241
1116	7	0.9882281096109473
1116	8	-1.4919614586552044
1116	9	-0.34615054533492184
1116	10	-0.34655934889682527
1116	11	0.26914759173016733
1116	12	0.47653045851136966
1116	13	0.18392016944137168
1116	14	0.3154423889915918
1116	15	-0.18113392761488
1116	16	0.1868315928310622
1116	17	0.13972169396858192
1116	18	0.28061863345698157
1116	19	0.8529406898470455
1116	20	0.6929205760457933
1116	21	0.5051299189532159
1116	22	0.47588492617260414
1116	23	0.5460008629551325
1116	24	1.5710943545750027
1116	25	0.7184194302180771
1201	1	-0.3681311139395511
1201	2	-0.3150018831925827
1201	3	0.2519556581365805
1201	4	-0.1263264661029595
1201	5	-0.0572029791163331
1201	6	0.451369206950222
1201	7	0.39101663541469733
1201	8	0.6701482781072414
1201	9	0.23664235627243357
1201	10	0.4667287078567455
1201	11	0.3845297402497394
1201	12	0.30244840320241195
1201	13	0.22309440135297365
1201	14	0.23136941497367047
1201	15	0.7334659710822677
1201	16	0.5740212779230589
1201	17	0.3750356279059375
1201	18	0.24178787215706876
1201	19	-0.20471561775396907
1201	20	0.5308150352594083
1201	21	0.7637069435865911
1201	22	0.5599764823702102
1201	23	0.6132335867729921
1201	24	-0.6797045292953641
1201	25	-0.0785618647200792
1202	1	-0.15835130620273574
1202	2	0.08489164682732309
1202	3	-0.08492461714232746
1202	4	0.051987604907893115
1202	5	0.21774340013282512
1202	6	1.223954730119015
1202	7	0.8832450983893771
1202	8	1.408450467685526
1202	9	1.1687731446131977
1202	10	0.9842846817484556
1202	11	0.5950756689302779
1202	12	0.9544420150036075
1202	13	0.671566017073495
1202	14	0.8814542823491109
1202	15	1.349248216827327
1202	16	1.2438216081342972
1202	17	0.902157698052676
1202	18	0.6309009309315812
1202	19	0.3131728819451354
1202	20	0.9126912214738567
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2211	2	0.1797135171424138
2211	3	0.04387623283144393
2211	4	0.20756848688881746
2211	5	0.4082452361507265
2211	6	-1.9962083146617111
2211	7	0.16431227880376653
2211	8	0.43532303286482943
2211	9	0.6176455713255962
2211	10	0.6662561575660106
2211	11	0.5738802468591899
2211	12	0.6808999783152032
2211	13	0.38407543681727674
2211	14	0.4834078639820092
2211	15	0.9136339962761753
2211	16	0.235667371978991
2211	17	0.40003143221219517
2211	18	0.23902694114085676
2211	19	0.4233952012125428
2211	20	0.3597722326018058
2211	21	0.06989715495083004
2211	22	-2.6495594245413336
2211	23	-1.214931858480604
2211	24	1.044290560920274
2211	25	0.5170430226872469
2212	1	-0.2550739924420675
2212	2	0.12693748196724997
2212	3	-2.9682856876501376
2212	4	-1.2013804496560212
2212	5	-0.3359397158466457
2212	6	0.5556798698650489
2212	7	0.6095343577419108
2212	8	0.40163841668374545
2212	9	0.4896710501752608
2212	10	0.6084431549143616
2212	11	0.3327575075284841
2212	12	0.26428291353227706
2212	13	0.23108107952756995
2212	14	-0.0077958533077406855
2212	15	-0.957889280803542
2212	16	0.0745458729260875
2212	17	0.1436632424585511
2212	18	0.21568646329060925
2212	19	0.9715149345306671
2212	20	-0.26177582234914354
2212	21	-0.6019020839394074
2212	22	0.11688076223121868
2212	23	0.3169795480331743
2212	24	1.4364841195596725
2212	25	0.9664172637784247
2213	1	0.047109941646066744
2213	2	-0.07731138572835405
2213	3	0.5850125525402632
2213	4	-7.008831531353451
2213	5	-1.3560363984435675
2213	6	-2.8528723897677466
2213	7	-0.8446980997450595
2213	8	-2.3840643743221897
2213	9	-0.340325540736238
2213	10	0.14971324153908938
2213	11	0.28770354441016366
2213	12	0.19649657656611197
2213	13	0.19029800554611853
2213	14	-0.07370401542790006
2213	15	-2.425252552003947
2213	16	-0.8361979436014266
2213	17	-0.33066325081749426
2213	18	-0.22549564344138417
2213	19	0.6485487946140509
2213	20	-0.839722195904784
2213	21	0.2005454722099742
2213	22	0.09568618374066062
2213	23	0.31975234393041063
2213	24	-0.17599462657334186
2213	25	0.05057622470518908
2214	1	0.6872381215950014
2214	2	0.24870377301357394
2214	3	0.10731578349263415
2214	4	0.3679766356091534
2214	5	0.516611331152049
2214	6	0.23487856917699546
2214	7	0.6972449607816849
2214	8	-2.650923564760401
2214	9	-1.2110633971089584
2214	10	-0.6460826324934459
2214	11	-0.5022805433197561
2214	12	-1.5656179917784883
2214	13	-0.6566676407846401
2214	14	-0.5118395543007352
2214	15	-1.8674322925177052
2214	16	-0.6088017841901419
2214	17	-0.3578594046174004
2214	18	-0.39191614421695675
2214	19	0.4833026754844596
2214	20	-1.45903015244064
2214	21	-0.7618622907332764
2214	22	-0.3314320119602243
2214	23	-0.7644003176673095
2214	24	-1.9465653439882615
2214	25	-1.0929869213550205
2215	1	0.6776493825643009
2215	2	0.06570681716377536
2215	3	0.31878017342641485
2215	4	0.5363799983975852
2215	5	0.7995278160511035
2215	6	1.1331787515969047
2215	7	0.6045630444084757
2215	8	-0.3965599487032456
2215	9	0.10683504148731515
2215	10	7.296010002235676E-4
2215	11	0.013828442793993645
2215	12	-0.2002025545291275
2215	13	0.03635668282503701
2215	14	0.061495923651847306
2215	15	0.14213305934462284
2215	16	0.3961658412867948
2215	17	0.007944068711204011
2215	18	-0.6212344893981978
2215	19	0.34230239197470524
2215	20	-3.4711151137277128
2215	21	-1.7893107124812389
2215	22	-0.7677664121939074
2215	23	-0.6949051082185065
2215	24	-0.2882055767908156
2215	25	0.05678593673832211
2216	1	-0.29176542485775414
2216	2	-1.1554087893660814
2216	3	-0.5307601890337672
2216	4	-0.7725386294152976
2216	5	-0.6885738305715954
2216	6	-1.9477884340937166
2216	7	-10.49777267832599
2216	8	-11.84867347230297
2216	9	-6.486778440049267
2216	10	-3.8019574754089724
2216	11	-2.7074698970679343
2216	12	-2.4661514967674627
2216	13	-2.1787353894904697
2216	14	-2.2353126559424226
2216	15	-3.1687852917249515
2216	16	-2.16560790864483
2216	17	-2.335838699742453
2216	18	-1.9628410073007814
2216	19	-1.5594955661009848
2216	20	-2.3356271973428093
2216	21	-5.274591140339272
2216	22	-3.7907917314485715
2216	23	-3.7514627983956395
2216	24	-3.8064497186360136
2216	25	-3.4110660984512897
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41267.0
3	3.0
4	358.0
5	2043.0
6	2583.0
7	2162.0
8	1777.0
9	1664.0
10	1480.0
11	1398.0
12	1456.0
13	1348.0
14	1419.0
15	1466.0
16	1553.0
17	1724.0
18	1992.0
19	2398.0
20	3059.0
21	4035.0
22	5022.0
23	6172.0
24	7159.0
25	8214.0
26	9532.0
27	10286.0
28	11599.0
29	13750.0
30	16267.0
31	19656.0
32	25826.0
33	34170.0
34	46020.0
35	65030.0
36	94591.0
37	156564.0
38	219771.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.54213725821366	18.220656655192233	12.089590504854865	26.14761558173925
2	14.46924023862883	19.97727344465137	42.028952077561655	23.524534239158147
3	19.145934605989677	24.051213815006726	29.504640128981503	27.298211450022098
4	11.546240162280665	16.435284882881547	39.013358794833124	33.00511616000466
5	12.76195049664638	37.920839745292135	36.68089121073547	12.636318547326017
6	32.09286532829676	37.54994633989583	18.2110873295458	12.146101002261608
7	27.73255396755318	31.431135954814298	22.688082198278536	18.148227879353982
8	24.326043515118638	38.02470697411934	21.253300608005137	16.39594890275688
9	25.907197555341327	15.484778483123247	20.23537115521036	38.37265280632507
10	13.640498223230205	29.94675754672359	35.50750124313245	20.90524298691376
11	34.71510318710461	22.61785666214363	24.56627948008105	18.100760670670706
12	22.72397661102367	25.08530767756972	32.53053978478456	19.66017592662206
13	28.955336452938546	22.372152976256157	25.78282163804738	22.889688932757917
14	19.801082140319306	22.608899161110855	28.18500185530557	29.40501684326427
15	22.7368467003525	31.91993490800663	23.079889266005317	22.26332912563555
16	21.281980389160925	28.741544856823413	28.648656514431647	21.327818239584015
17	20.958269720718896	29.189610660483865	28.412799080340456	21.439320538456784
18	20.823400163457737	28.25575557975355	30.23483104920951	20.6860132075792
19	23.242965312844095	27.380588580547833	27.575949397789064	21.800496708819004
20	22.787341268686397	28.141400618918734	28.431458145377995	20.63979996701687
21	22.320051413881746	27.437430275985836	27.432337391473055	22.81018091865936
22	22.0637061001962	28.928457962171166	27.7816851025026	21.226150835130028
23	21.481363812193887	28.61231127047665	28.050867085668134	21.85545783166133
24	22.929265125122626	27.237164881510324	28.630083997133404	21.203485996233653
25	21.957607799388853	28.056458262598827	28.448246592617743	21.537687345394577
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	215.0
1	215.0
2	322.5
3	430.0
4	430.0
5	430.0
6	639.5
7	849.0
8	849.0
9	849.0
10	1239.5
11	1630.0
12	1630.0
13	1630.0
14	2809.5
15	3989.0
16	3989.0
17	3989.0
18	6959.0
19	9929.0
20	9929.0
21	9929.0
22	15471.0
23	21013.0
24	21013.0
25	21013.0
26	30544.5
27	40076.0
28	40076.0
29	40076.0
30	52764.5
31	65453.0
32	65453.0
33	65453.0
34	74645.5
35	83838.0
36	83838.0
37	83838.0
38	90610.0
39	97382.0
40	97382.0
41	97382.0
42	101935.0
43	106488.0
44	106488.0
45	106488.0
46	108037.0
47	109586.0
48	109586.0
49	109586.0
50	104115.5
51	98645.0
52	98645.0
53	98645.0
54	86796.5
55	74948.0
56	74948.0
57	74948.0
58	63111.5
59	51275.0
60	51275.0
61	51275.0
62	41267.0
63	31259.0
64	31259.0
65	31259.0
66	23767.5
67	16276.0
68	16276.0
69	16276.0
70	11800.5
71	7325.0
72	7325.0
73	7325.0
74	5050.5
75	2776.0
76	2776.0
77	2776.0
78	1875.5
79	975.0
80	975.0
81	975.0
82	646.5
83	318.0
84	318.0
85	318.0
86	204.5
87	91.0
88	91.0
89	91.0
90	65.0
91	39.0
92	39.0
93	39.0
94	22.5
95	6.0
96	6.0
97	6.0
98	4.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22126200573705102
2	0.1343333163598096
3	0.13700058437417406
4	0.06825781327669025
5	0.022186820301304293
6	0.02170186248051076
7	0.3160712597021874
8	0.31728365425417127
9	0.019277073376543077
10	0.03443200527634109
11	0.017822199914162465
12	0.018428397190154388
13	0.016609805362178622
14	0.018428397190154388
15	0.016367326451781856
16	0.02036822847332853
17	0.019519552286939846
18	0.01648856590698024
19	0.022550538666899447
20	0.01818591827975762
21	0.016246086996583472
22	0.018428397190154388
23	0.018792115555749538
24	0.01733724209336893
25	0.016246086996583472
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	824814.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.28309277138679
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.16687277503868	80.38725926868653
2	5.356929726709754	9.244249291589947
3	0.7759732681013495	2.008601204391148
4	0.2512941616558264	0.8672974985223014
5	0.11932408423970377	0.5147825515157567
6	0.06935907946261333	0.3590709532686392
7	0.04973277063067694	0.30037680834733593
8	0.03289500572189667	0.2270626264334168
9	0.024819584468922715	0.19273594583513964
>10	0.12877176822428177	2.0621355791471307
>50	0.011945022378367842	0.702027775202385
>100	0.01053728499604884	1.8808100372795604
>500	9.83479873013212E-4	0.5773959346229167
>1k	5.619884988646926E-4	0.6761945251578059
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	1760	0.21338144114915603	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1286	0.155913939385122	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1283	0.15555022101952684	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1249	0.15142807954278176	No Hit
GTATCAACGCAGAGTACTTTTTTTT	889	0.10778187567136348	No Hit
CTTTAGGACGTGAAATATGGCGAGG	877	0.10632700220898288	No Hit
CTGTAGGACCTGGAATATGGCGAGA	859	0.10414469201541197	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.4247891039676824E-4	0.0
6	0.0	0.0	0.0	2.4247891039676824E-4	0.0
7	0.0	0.0	0.0	2.4247891039676824E-4	0.0
8	0.0	0.0	0.0	2.4247891039676824E-4	0.0
9	0.0	0.0	0.0	2.4247891039676824E-4	0.0
10	0.0	0.0	0.0	2.4247891039676824E-4	0.0
11	0.0	0.0	0.0	2.4247891039676824E-4	0.0
12	0.0	0.0	0.0	2.4247891039676824E-4	0.0
13	0.0	0.0	0.0	2.4247891039676824E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCCA	30	7.822201E-4	18.96234	12
GGTCTAA	45	6.550726E-4	14.838472	1
ATCCCGT	55	1.989582E-4	13.792466	10
TAGGACC	340	0.0	13.724798	4
TCCAACG	90	3.7232894E-8	13.695855	18
TATGTCG	70	1.1094425E-4	12.190816	16
GGATAGG	105	2.5730697E-7	11.810213	1
TGGCGAG	730	0.0	11.559936	18
ATGTCGA	75	2.1088989E-4	11.378094	17
TGTCGAG	75	2.1088989E-4	11.378094	18
GTAGGAC	905	0.0	11.16732	3
TAATTCT	145	1.1641532E-9	11.165282	4
CCAACGA	85	5.4207558E-5	11.154994	19
CTAGTAC	60	0.0057159318	11.123425	3
TAGGACT	155	2.9831426E-10	11.059351	4
GGCGAGA	490	0.0	11.029786	19
GGACCTG	610	0.0	10.923754	6
GATATAC	280	0.0	10.901734	1
AGGACCT	655	0.0	10.900592	5
TGTAGGA	1015	0.0	10.897079	2
>>END_MODULE