FastQCFastQC Report
Thu 26 May 2016
SRR1926769_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926769_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824814
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17090.20719822893403844No Hit
CTGTAGGACGTGGAATATGGCAAGA15280.18525388754313094No Hit
GTATCAACGCAGAGTACTTTTTTTT14630.17737332295523597No Hit
GTCCTACAGTGTGCATTTCTCATTT13890.16840160327055556No Hit
CTTTAGGACGTGAAATATGGCGAGG12280.1488820509836157No Hit
GTCCTAAAGTGTGTATTTCTCATTT12200.14791213534202863No Hit
GGTATCAACGCAGAGTACTTTTTTT9650.11699607426644068No Hit
CTGTAGGACCTGGAATATGGCGAGA9220.11178277769291016No Hit
TATCAACGCAGAGTACTTTTTTTTT8290.10050750835946043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3400.013.7234474
TCTAGAC500.001471474313.3321583
CCTAAAC801.9427334E-613.0940853
TAGGACT1155.125912E-912.420514
CTAGGAC1551.8189894E-1212.2877043
GTGTAGC2300.012.0174611
TATACTT1209.640644E-911.9029895
TCCAACG1052.752986E-711.74887918
TTAGGAC4150.011.7029283
GCCTAAG907.1824143E-611.649111
CTAAACT1156.8277586E-811.5924764
TGGCGAG7800.011.557718
GTCCTAA3550.011.5447011
GGCGAGA5100.011.350162519
GGCGAGG3200.011.26875719
GTCCTAC9650.011.2595261
TGTAGGA9650.011.1600792
GGACAGT1201.2310375E-711.1094576
CTTTAGG4650.011.0683631
CCAACGA951.3747171E-510.98781919
Produced by FastQC (version 0.11.5)