FastQCFastQC Report
Thu 26 May 2016
SRR1926768_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926768_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730048
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15750.21573923906373277No Hit
CTGTAGGACGTGGAATATGGCAAGA12550.17190650477776803No Hit
GTCCTAAAGTGTGTATTTCTCATTT12280.16820811782238976No Hit
GTCCTACAGTGTGCATTTCTCATTT11200.15341457000087663No Hit
CTTTAGGACGTGAAATATGGCGAGG9430.1291695888489524No Hit
GTATCAACGCAGAGTACTTTTTTTT8140.11149951783992285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAC508.425046E-515.2581043
TCCAACG551.994143E-413.788341518
TTATACT1257.2759576E-1213.7181134
GCCTCGA500.001522823113.27127916
CTCGAAC500.001522823113.27127918
TAGGACC3000.013.01950554
CCTAGGC550.002979367312.1371283
ACTACTC802.8225488E-511.8908958
ATTTCTC8700.011.65867615
CTAGGAC751.9988057E-411.4435783
ATAGGAC1251.7245839E-811.4435773
CTAAAGT4250.011.4317624
GTCCTAA4200.011.3668551
GCATTTC5600.011.34152713
GTATAAT1354.3473847E-911.3163361
GTATAAC855.0467625E-511.2331281
TAGATAG855.194163E-511.2014415
GGACGTG5800.011.1628156
CCGGGAA600.005965817311.059417
TGTTATA951.30111075E-511.0420482
Produced by FastQC (version 0.11.5)