##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926768_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 730048 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.919582000087665 31.0 28.0 31.0 20.0 33.0 2 28.53046512010169 31.0 29.0 31.0 23.0 34.0 3 28.465822795213466 31.0 28.0 33.0 16.0 34.0 4 32.25431752432717 35.0 33.0 37.0 23.0 37.0 5 32.07760037696151 35.0 32.0 37.0 22.0 37.0 6 31.761075984044886 35.0 32.0 37.0 17.0 37.0 7 31.854165753484704 35.0 32.0 37.0 17.0 37.0 8 31.684649502498466 35.0 32.0 37.0 17.0 37.0 9 33.728519220653986 38.0 34.0 39.0 22.0 39.0 10 33.81387936135706 38.0 34.0 39.0 23.0 39.0 11 33.99695910405891 38.0 35.0 39.0 25.0 39.0 12 33.836446370649604 38.0 34.0 39.0 23.0 39.0 13 33.95265516787937 38.0 35.0 39.0 25.0 39.0 14 35.023566943543436 39.0 35.0 41.0 25.0 41.0 15 34.59032693740686 39.0 34.0 41.0 18.0 41.0 16 34.69385985579031 39.0 34.0 41.0 19.0 41.0 17 34.92626923161217 39.0 35.0 41.0 24.0 41.0 18 34.9820888489524 39.0 35.0 41.0 24.0 41.0 19 34.33832432935917 39.0 34.0 41.0 18.0 41.0 20 34.59773877882002 39.0 34.0 41.0 18.0 41.0 21 34.59841407688262 39.0 34.0 41.0 18.0 41.0 22 34.63808955027615 39.0 35.0 41.0 18.0 41.0 23 34.64360014683966 39.0 35.0 41.0 18.0 41.0 24 33.335157414307005 39.0 33.0 41.0 2.0 41.0 25 33.939531373279564 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.30088657104626293 1101 2 -0.4142668070058164 1101 3 -1.299653409332219 1101 4 -0.5429504921983757 1101 5 -0.41894245696137133 1101 6 -2.6449390721158927 1101 7 -0.8117689772656647 1101 8 -0.2853781340788686 1101 9 -0.2867318321902488 1101 10 -9.121127966856651 1101 11 -3.896397191388129 1101 12 -1.6417950170461637 1101 13 -0.6516605487746716 1101 14 -0.7454544116874757 1101 15 -2.175978194891293 1101 16 -0.4125094624558585 1101 17 -0.21046868027723775 1101 18 -0.20003294720265075 1101 19 -1.0128501539358155 1101 20 -0.20513519490638288 1101 21 -0.2792879948571567 1101 22 0.029141063201578277 1101 23 -0.058455043130720696 1101 24 -0.35711391634654177 1101 25 -0.2929313185315294 1102 1 -0.20732392064496352 1102 2 -0.22051689394298535 1102 3 -0.2597092577694369 1102 4 -0.055258187181060237 1102 5 -0.20330958914759734 1102 6 -0.8500873173760795 1102 7 0.19935376410668937 1102 8 0.505457123463799 1102 9 0.334446409870651 1102 10 0.5464347322294643 1102 11 0.3495324103700881 1102 12 0.22844691170708842 1102 13 0.0050784727996529 1102 14 -0.19030842679367765 1102 15 0.3557512635944704 1102 16 0.20787591262585892 1102 17 0.0852141937960127 1102 18 -0.19333356897728038 1102 19 0.402473913054493 1102 20 0.13367981670842255 1102 21 0.07262844809120139 1102 22 0.05920915239215674 1102 23 -0.3433177171789339 1102 24 -3.882747340558197 1102 25 -1.9757775718873063 1103 1 -0.24399758725975573 1103 2 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-2.0881532029665166 2216 14 -2.3189183371657975 2216 15 -3.378898840293882 2216 16 -2.172831287161621 2216 17 -2.629510657158683 2216 18 -2.357723838868047 2216 19 -1.9707527416238406 2216 20 -2.7733074503090904 2216 21 -5.138880498275849 2216 22 -3.723460942431963 2216 23 -3.730199833275531 2216 24 -4.271723279476081 2216 25 -3.3377834089019167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34920.0 3 3.0 4 284.0 5 1751.0 6 2146.0 7 1802.0 8 1503.0 9 1334.0 10 1261.0 11 1222.0 12 1224.0 13 1223.0 14 1222.0 15 1346.0 16 1371.0 17 1494.0 18 1683.0 19 2162.0 20 2677.0 21 3403.0 22 4460.0 23 5403.0 24 6436.0 25 7216.0 26 8417.0 27 9259.0 28 10418.0 29 12090.0 30 14318.0 31 17839.0 32 23306.0 33 30199.0 34 41114.0 35 57365.0 36 84068.0 37 140535.0 38 193574.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.66114232055485 18.115160101700685 12.132007709922407 26.091689867822055 2 14.571485788432682 19.952020014978313 41.82515972753733 23.651334469051672 3 19.19898274814375 24.00116320061233 29.512100441275514 27.28775360996841 4 11.561120706198427 16.46366203361022 38.77690051265181 33.19831674753955 5 12.873190307019286 37.90261258903751 36.47390219608735 12.750294907855853 6 32.15538359520143 37.45293772709088 18.245365016906796 12.146313660800894 7 27.910792090499502 31.33640109757991 22.628036104168416 18.124770707752173 8 24.456312653886737 37.867679387970455 21.0545913207879 16.62141663735491 9 25.986228288181152 15.440840003178508 20.009946540768485 38.56298516787185 10 13.9738577675098 29.58300960430378 35.36710529380893 21.076027334377486 11 34.65920844789864 22.607280394227963 24.625684823127603 18.10782633474579 12 23.030058088557652 24.790250986409472 32.350805567733445 19.82888535729943 13 29.03759213086007 22.188125051374087 25.82979422966271 22.94448858810313 14 20.09132482648831 22.43732892007639 27.879828255621835 29.591517997813465 15 22.669733593812076 31.929013149061973 22.98469185054073 22.416561406585217 16 21.4539072678923 28.543969179719664 28.526021736956253 21.476101815431786 17 21.176367432607947 29.17833480383829 28.170125550069326 21.475172213484438 18 20.926896831387744 28.171591950582307 30.128736432122594 20.772774785907355 19 23.4604234143882 27.312173574689396 27.3249154650927 21.9024875458297 20 23.025242319416378 27.99109497551118 28.303181833750042 20.680480871322395 21 22.39185680916794 27.292978141889346 27.329008747354216 22.986156301588498 22 22.30979686731505 28.86876179072415 27.543953627604235 21.277487714356564 23 21.617996616044334 28.356041456881965 28.02298897816869 22.002972948905008 24 22.97885282464668 27.21623384096053 28.512518974370217 21.292394360022577 25 22.041261434299955 27.897157402549006 28.320485086946572 21.741096076204464 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 238.0 1 238.0 2 291.5 3 345.0 4 345.0 5 345.0 6 525.5 7 706.0 8 706.0 9 706.0 10 1088.0 11 1470.0 12 1470.0 13 1470.0 14 2478.0 15 3486.0 16 3486.0 17 3486.0 18 5997.0 19 8508.0 20 8508.0 21 8508.0 22 13391.5 23 18275.0 24 18275.0 25 18275.0 26 26452.0 27 34629.0 28 34629.0 29 34629.0 30 45584.0 31 56539.0 32 56539.0 33 56539.0 34 64561.5 35 72584.0 36 72584.0 37 72584.0 38 79303.5 39 86023.0 40 86023.0 41 86023.0 42 89658.0 43 93293.0 44 93293.0 45 93293.0 46 95217.0 47 97141.0 48 97141.0 49 97141.0 50 92516.0 51 87891.0 52 87891.0 53 87891.0 54 77236.5 55 66582.0 56 66582.0 57 66582.0 58 56638.0 59 46694.0 60 46694.0 61 46694.0 62 37733.0 63 28772.0 64 28772.0 65 28772.0 66 22164.5 67 15557.0 68 15557.0 69 15557.0 70 11330.5 71 7104.0 72 7104.0 73 7104.0 74 4927.0 75 2750.0 76 2750.0 77 2750.0 78 1879.0 79 1008.0 80 1008.0 81 1008.0 82 661.5 83 315.0 84 315.0 85 315.0 86 210.5 87 106.0 88 106.0 89 106.0 90 64.5 91 23.0 92 23.0 93 23.0 94 13.5 95 4.0 96 4.0 97 4.0 98 4.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22409485403699483 2 0.13615543087577803 3 0.14040172700973086 4 0.06958446567896905 5 0.02177938984833874 6 0.02246427632155694 7 0.3102535723678443 8 0.30682914000175326 9 0.01999868501797142 10 0.037120846848426405 11 0.015752388884018587 12 0.017533093714385904 13 0.014793547821513106 14 0.017259139125098626 15 0.015067502410800387 16 0.019039843955465943 17 0.01917682125010958 18 0.014656570526869465 19 0.023560094678706057 20 0.016848207241167704 21 0.015478434294731306 22 0.017944025598316822 23 0.0182179801876041 24 0.015889366178662226 25 0.016026343473305865 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 730048.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.25676377381885 #Duplication Level Percentage of deduplicated Percentage of total 1 93.26285692863759 80.44552218964958 2 5.2314945366919305 9.025035768709195 3 0.7877949990392978 2.0385794140298565 4 0.252194999852507 0.8701409810886389 5 0.12427891594348141 0.5359948547301583 6 0.07416332819823818 0.38382532086453774 7 0.050161534504014865 0.3028740142571552 8 0.029136084179915058 0.2010547464320827 9 0.026514167634177667 0.20583236648526265 >10 0.1364663995717076 2.15902599355771 >50 0.012707763788160633 0.7600185813185772 >100 0.011118492696565958 2.0238593742612556 >500 4.765068267384441E-4 0.33881696561043023 >1k 6.353424356512589E-4 0.7094194290055834 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1575 0.21573923906373277 No Hit CTGTAGGACGTGGAATATGGCAAGA 1255 0.17190650477776803 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1228 0.16820811782238976 No Hit GTCCTACAGTGTGCATTTCTCATTT 1120 0.15341457000087663 No Hit CTTTAGGACGTGAAATATGGCGAGG 943 0.1291695888489524 No Hit GTATCAACGCAGAGTACTTTTTTTT 814 0.11149951783992285 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.3697729464363988E-4 0.0 0.0 0.0 6 0.0 1.3697729464363988E-4 0.0 0.0 0.0 7 0.0 1.3697729464363988E-4 0.0 0.0 0.0 8 0.0 1.3697729464363988E-4 0.0 0.0 0.0 9 0.0 1.3697729464363988E-4 0.0 0.0 0.0 10 0.0 1.3697729464363988E-4 0.0 0.0 0.0 11 1.3697729464363988E-4 1.3697729464363988E-4 0.0 0.0 0.0 12 1.3697729464363988E-4 1.3697729464363988E-4 0.0 0.0 0.0 13 1.3697729464363988E-4 1.3697729464363988E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTATAC 50 8.425046E-5 15.258104 3 TCCAACG 55 1.994143E-4 13.7883415 18 TTATACT 125 7.2759576E-12 13.718113 4 GCCTCGA 50 0.0015228231 13.271279 16 CTCGAAC 50 0.0015228231 13.271279 18 TAGGACC 300 0.0 13.0195055 4 CCTAGGC 55 0.0029793673 12.137128 3 ACTACTC 80 2.8225488E-5 11.890895 8 ATTTCTC 870 0.0 11.658676 15 CTAGGAC 75 1.9988057E-4 11.443578 3 ATAGGAC 125 1.7245839E-8 11.443577 3 CTAAAGT 425 0.0 11.431762 4 GTCCTAA 420 0.0 11.366855 1 GCATTTC 560 0.0 11.341527 13 GTATAAT 135 4.3473847E-9 11.316336 1 GTATAAC 85 5.0467625E-5 11.233128 1 TAGATAG 85 5.194163E-5 11.201441 5 GGACGTG 580 0.0 11.162815 6 CCGGGAA 60 0.0059658173 11.0594 17 TGTTATA 95 1.30111075E-5 11.042048 2 >>END_MODULE