FastQCFastQC Report
Thu 26 May 2016
SRR1926768_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926768_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730048
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA16090.22039646708161653No Hit
GTCCTACAGTGGACATTTCTAAATT15440.21149294292977996No Hit
GTATCAACGCAGAGTACTTTTTTTT14170.19409682651003768No Hit
GTCCTAAAGTGTGTATTTCTCATTT12340.1690299815902516No Hit
CTTTAGGACGTGAAATATGGCGAGG11660.1597155255544841No Hit
GTCCTACAGTGTGCATTTCTCATTT11340.1553322521258876No Hit
CTGTAGGACCTGGAATATGGCGAGA9230.12643004295607962No Hit
GGTATCAACGCAGAGTACTTTTTTT9110.12478631542035593No Hit
TATCAACGCAGAGTACTTTTTTTTT7340.10054133426843166No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTCG351.00210375E-418.977539
TAGGACC3450.014.352054
TGTAGGA9150.013.2254672
GTAGGAC9050.012.8372143
GTAAGAC604.0059464E-412.69691853
TCCAACG1052.0179868E-812.65255418
CTGTAGG9150.012.6023751
GGACGTG7850.012.251286
CTAATAC701.0653722E-412.2434563
CTTAGGC550.003011461812.1197853
AGGACGT8050.012.0651855
TGGCGAG6750.011.94963418
GACGTGA3750.011.8933997
AGGACCT5800.011.8204565
TAGGACG8300.011.81654
TAGAACT1052.6316957E-711.7891854
GGCGAGG2750.011.732367519
GAACTGC657.8601664E-411.7194256
GGACCTG5600.011.56246956
GTTCTGC752.0113561E-411.4358751
Produced by FastQC (version 0.11.5)