FastQCFastQC Report
Thu 26 May 2016
SRR1926767_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926767_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761888
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16760.21997983955647024No Hit
CTGTAGGACGTGGAATATGGCAAGA13600.1785039270863959No Hit
GTCCTAAAGTGTGTATTTCTCATTT13440.17640388088537948No Hit
GTCCTACAGTGTGCATTTCTCATTT11880.15592843042546936No Hit
CTTTAGGACGTGAAATATGGCGAGG9810.1287590826998194No Hit
GTATCAACGCAGAGTACTTTTTTTT8150.10697110336427401No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATC456.8577484E-414.748878513
GCCGGTT551.9908677E-413.7911611
TAGGACC3400.013.4428994
GTCCTAG1500.013.3625891
GGGTTAT751.4066398E-512.72627451
TGCCGGT604.1535238E-412.64272610
TGTAGGA8850.012.3945342
GGACTGT1001.4054604E-712.3721436
GTAGGAC8300.012.1815763
GTATTAG550.002959999512.1478091
ACGAAAT550.003111250712.06726512
CTACACT1209.640644E-911.9025664
GTCCTAC7950.011.885861
TCCTAGA2100.011.8094522
GCTATAC657.748341E-411.7395733
CCGGTTT658.147874E-411.66944212
CTGTAGG8900.011.582341
TCCTACA9150.011.4669322
CCTACAC1501.6370905E-1011.4460833
GACGTGG2700.011.2794487
Produced by FastQC (version 0.11.5)