##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926767_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 761888 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.688193015246334 31.0 28.0 31.0 16.0 33.0 2 28.273649145281198 31.0 28.0 31.0 16.0 34.0 3 28.208000388508548 31.0 28.0 33.0 16.0 34.0 4 31.96263624049729 35.0 32.0 37.0 19.0 37.0 5 31.78288803603679 35.0 32.0 37.0 19.0 37.0 6 31.464880664874627 35.0 32.0 37.0 17.0 37.0 7 31.53926955143013 35.0 32.0 37.0 17.0 37.0 8 31.363283054727205 35.0 32.0 37.0 15.0 37.0 9 33.37690579192742 37.0 33.0 39.0 17.0 39.0 10 33.459352293250454 38.0 34.0 39.0 17.0 39.0 11 33.63254310344828 38.0 34.0 39.0 19.0 39.0 12 33.4755646499223 38.0 34.0 39.0 18.0 39.0 13 33.59652074845646 38.0 34.0 39.0 19.0 39.0 14 34.6580494770885 39.0 35.0 41.0 19.0 41.0 15 34.22624585240875 39.0 34.0 41.0 17.0 41.0 16 34.326857753370575 39.0 34.0 41.0 17.0 41.0 17 34.55899686043093 39.0 34.0 41.0 18.0 41.0 18 34.61252178797933 39.0 35.0 41.0 18.0 41.0 19 33.979122915704146 39.0 34.0 41.0 10.0 41.0 20 34.24015340837499 39.0 34.0 41.0 16.0 41.0 21 34.230183439035656 39.0 34.0 41.0 11.0 41.0 22 34.265751659036496 39.0 34.0 41.0 10.0 41.0 23 34.276163950606914 39.0 34.0 41.0 8.0 41.0 24 32.966866521063466 39.0 32.0 41.0 2.0 41.0 25 33.5628661955563 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.16835185662227303 1101 2 -0.22269187670554658 1101 3 -0.6921749810843565 1101 4 -0.17406032334045918 1101 5 -0.08258299747757292 1101 6 -1.8972553757030752 1101 7 -0.3574662971768916 1101 8 0.13552426463982314 1101 9 0.031310548456694676 1101 10 -8.970444959432253 1101 11 -3.9497706011911724 1101 12 -1.4577882708148167 1101 13 -0.42461620060327476 1101 14 -0.4455433350930633 1101 15 -1.7094886399478852 1101 16 -0.15083672811702087 1101 17 0.0051141412483914905 1101 18 0.07337258111275702 1101 19 -0.6050811525555488 1101 20 0.20708805635869254 1101 21 0.1803779591384611 1101 22 0.2186935350262189 1101 23 0.2623063953570224 1101 24 0.4363544217307407 1101 25 0.30605772022274635 1102 1 -0.20267801779236905 1102 2 -0.07897878250856394 1102 3 -0.05854073972673035 1102 4 -0.05054830027004087 1102 5 -0.007608956516495624 1102 6 -0.8515361477044152 1102 7 0.3455333872190174 1102 8 0.6670786509344104 1102 9 0.4309592883290563 1102 10 0.62951119328158 1102 11 0.38606829135165555 1102 12 0.4634552574943882 1102 13 0.13949840945702618 1102 14 0.13368172435373538 1102 15 0.7036202531008726 1102 16 0.6214561235707379 1102 17 0.4326179537940149 1102 18 0.16376303878687537 1102 19 0.601550388098353 1102 20 0.63165265521625 1102 21 0.4193119400624781 1102 22 0.5027733563605707 1102 23 0.1012543495920184 1102 24 -3.226481630375254 1102 25 -1.6143835473011094 1103 1 0.1880939097128902 1103 2 0.1423906659957339 1103 3 0.5935202388984351 1103 4 0.40724429700124176 1103 5 0.0014492140757838001 1103 6 0.9155048417415905 1103 7 0.7101002720162235 1103 8 0.5868673262626523 1103 9 0.5263760988247839 1103 10 0.6054140113518969 1103 11 0.19547214131897306 1103 12 0.06689589342693125 1103 13 0.15476754729055386 1103 14 0.2168430748535286 1103 15 0.4021912654860884 1103 16 0.541325587988041 1103 17 0.3113217051007169 1103 18 0.10078671978259024 1103 19 -1.1539573468448836 1103 20 -0.17667890320527846 1103 21 0.22264191942453948 1103 22 0.1384268054426343 1103 23 0.243266879245283 1103 24 1.7000835358176971 1103 25 0.9342306750059848 1104 1 -0.46323187454068204 1104 2 -0.27304746473560826 1104 3 -1.2988102748703199 1104 4 -0.1814422697590885 1104 5 -0.4674341094292629 1104 6 -1.7030223157664643 1104 7 -0.21799167937234998 1104 8 0.4616087865949652 1104 9 0.3599207204436752 1104 10 0.3795138078625726 1104 11 0.296037407116458 1104 12 0.303256259723625 1104 13 0.13033450419013093 1104 14 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0.8014182583202576 2215 2 0.033037566265274876 2215 3 0.2765252841676329 2215 4 0.6307407376674945 2215 5 0.9666389300202205 2215 6 1.109720751617882 2215 7 0.646804259852285 2215 8 -0.5414960713460317 2215 9 0.11317615411540771 2215 10 -0.09063063582483011 2215 11 -0.1914682139232653 2215 12 -0.23896422130111006 2215 13 3.263281323526712E-4 2215 14 0.13971749602983863 2215 15 -0.07879014304248955 2215 16 0.22755407648067916 2215 17 -0.37017321507345 2215 18 -0.6342136257837936 2215 19 0.27487463877955065 2215 20 -3.373407080688171 2215 21 -1.9787555510295363 2215 22 -0.9083179592266575 2215 23 -0.9483655763494312 2215 24 -0.7875624659792493 2215 25 -0.5978503257542656 2216 1 -0.1348490227857333 2216 2 -1.0511867040880247 2216 3 -0.4316514852994615 2216 4 -0.8303002643412469 2216 5 -0.5975711609640655 2216 6 -2.3236917498591403 2216 7 -10.248338124644938 2216 8 -11.454549362141258 2216 9 -6.234876545872776 2216 10 -3.7664087286996946 2216 11 -2.7275925197249897 2216 12 -2.3685010285798533 2216 13 -2.135917320691938 2216 14 -2.2087865805387565 2216 15 -3.27503969993721 2216 16 -2.1904513589441166 2216 17 -2.5288226301744814 2216 18 -2.15578517251663 2216 19 -1.5259394933249055 2216 20 -2.4801162854618894 2216 21 -5.253476099298471 2216 22 -3.5626426667771725 2216 23 -3.541983678677216 2216 24 -3.791018687177406 2216 25 -3.286097992066445 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43085.0 3 7.0 4 367.0 5 1990.0 6 2488.0 7 2042.0 8 1670.0 9 1520.0 10 1367.0 11 1330.0 12 1308.0 13 1246.0 14 1370.0 15 1323.0 16 1464.0 17 1625.0 18 1807.0 19 2296.0 20 3002.0 21 3886.0 22 4691.0 23 5535.0 24 6644.0 25 7533.0 26 8742.0 27 9347.0 28 10564.0 29 12419.0 30 14940.0 31 18291.0 32 23712.0 33 31311.0 34 42120.0 35 58845.0 36 86864.0 37 144115.0 38 201022.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.597154290795906 18.192832580423644 12.000994524851281 26.209018603929163 2 14.656113931686724 19.840263275141712 41.66153005019208 23.84209274297948 3 19.098765188704665 23.896457228476233 29.467631383527525 27.53714619929158 4 11.61830958166415 16.3742037170815 38.68943324358048 33.31805345767387 5 12.905114548612435 37.83327141279904 36.52112496701583 12.740489071572696 6 32.25964833052834 37.388183338628245 18.12736137622122 12.224806954622194 7 28.00265410530584 31.33871702445305 22.53343303781837 18.12519583242274 8 24.574830939433074 37.71344019988442 21.047234278387496 16.66449458229501 9 26.07084392832904 15.366408536033049 20.089687158274465 38.473060377363446 10 13.940303485075873 29.550733371935312 35.41101259291063 21.09795055007819 11 34.66376819584631 22.45776673348377 24.69843695235245 18.18002811831747 12 23.14166105472919 24.783497359435735 32.169069626094 19.905771959741074 13 29.179903070145503 22.19646410812457 25.58244550977437 23.041187311955557 14 20.03833177993358 22.483820575764337 27.792247003688775 29.68560064061331 15 22.5824998785753 32.106134375791726 22.833750552974642 22.477615192658334 16 21.457742864156753 28.463031980182553 28.51803668643248 21.561188469228217 17 21.146651333874182 29.18363381930934 28.219558237043923 21.450156609772556 18 21.030253625328665 28.09201449737658 30.00408247384788 20.873649403446866 19 23.48308514730343 27.292717732205894 27.300725850600017 21.92347126989066 20 23.209236383685365 27.955944707720175 28.177402628089844 20.657416280504613 21 22.428402466286773 27.25989868638856 27.206734637887198 23.104964209437473 22 22.442453480358374 28.754159692822682 27.48554363164977 21.31784319516918 23 21.48593797586667 28.513011835624496 27.987917581205828 22.01313260730301 24 23.02756544808457 27.081323257025915 28.562314662760063 21.328796632129453 25 21.97417906627198 27.9191635435195 28.331878417138697 21.77477897306983 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 189.0 1 189.0 2 261.0 3 333.0 4 333.0 5 333.0 6 539.5 7 746.0 8 746.0 9 746.0 10 1119.0 11 1492.0 12 1492.0 13 1492.0 14 2550.0 15 3608.0 16 3608.0 17 3608.0 18 6166.5 19 8725.0 20 8725.0 21 8725.0 22 13678.0 23 18631.0 24 18631.0 25 18631.0 26 27159.5 27 35688.0 28 35688.0 29 35688.0 30 46976.5 31 58265.0 32 58265.0 33 58265.0 34 66915.0 35 75565.0 36 75565.0 37 75565.0 38 82377.5 39 89190.0 40 89190.0 41 89190.0 42 93179.0 43 97168.0 44 97168.0 45 97168.0 46 99454.5 47 101741.0 48 101741.0 49 101741.0 50 96934.0 51 92127.0 52 92127.0 53 92127.0 54 81419.5 55 70712.0 56 70712.0 57 70712.0 58 60014.0 59 49316.0 60 49316.0 61 49316.0 62 39912.0 63 30508.0 64 30508.0 65 30508.0 66 23320.0 67 16132.0 68 16132.0 69 16132.0 70 11819.0 71 7506.0 72 7506.0 73 7506.0 74 5155.0 75 2804.0 76 2804.0 77 2804.0 78 1890.0 79 976.0 80 976.0 81 976.0 82 648.0 83 320.0 84 320.0 85 320.0 86 210.5 87 101.0 88 101.0 89 101.0 90 67.5 91 34.0 92 34.0 93 34.0 94 20.0 95 6.0 96 6.0 97 6.0 98 5.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22654248393464654 2 0.1326966693267252 3 0.13689676172875803 4 0.07061405350917721 5 0.020869209122600697 6 0.01968793313452896 7 0.303194170271746 8 0.29518774413037085 9 0.016931622495694906 10 0.035307026754588605 11 0.014044058969297325 12 0.015619093620059641 13 0.013125288756352639 14 0.015487840732496115 15 0.012994035868789113 16 0.017719139821076063 17 0.01601285228275022 18 0.012469024318535008 19 0.02113171489772775 20 0.015487840732496115 21 0.012469024318535008 22 0.014831576294678484 23 0.015750346507623168 24 0.013256541643916165 25 0.013519047419043218 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 761888.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.46450715734547 #Duplication Level Percentage of deduplicated Percentage of total 1 93.29536880087839 80.66738083430735 2 5.219814896272504 9.026574449175447 3 0.7834590493788247 2.032242017475074 4 0.2427314985071804 0.8395063755994916 5 0.12986276193555088 0.5614259854424544 6 0.07302539694276083 0.37884629739752024 7 0.04717271135206378 0.2855135666832337 8 0.031789684527807574 0.21989435243074976 9 0.026531087077500473 0.20645976316542258 >10 0.1258062883275539 2.028650222835461 >50 0.01290267672759597 0.7632007631701043 >100 0.010320921975931832 1.849747842955116 >500 6.07113048143139E-4 0.4098448008513477 >1k 6.07113048143139E-4 0.7307127285111445 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1676 0.21997983955647024 No Hit CTGTAGGACGTGGAATATGGCAAGA 1360 0.1785039270863959 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1344 0.17640388088537948 No Hit GTCCTACAGTGTGCATTTCTCATTT 1188 0.15592843042546936 No Hit CTTTAGGACGTGAAATATGGCGAGG 981 0.1287590826998194 No Hit GTATCAACGCAGAGTACTTTTTTTT 815 0.10697110336427401 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAAATC 45 6.8577484E-4 14.7488785 13 GCCGGTT 55 1.9908677E-4 13.79116 11 TAGGACC 340 0.0 13.442899 4 GTCCTAG 150 0.0 13.362589 1 GGGTTAT 75 1.4066398E-5 12.7262745 1 TGCCGGT 60 4.1535238E-4 12.642726 10 TGTAGGA 885 0.0 12.394534 2 GGACTGT 100 1.4054604E-7 12.372143 6 GTAGGAC 830 0.0 12.181576 3 GTATTAG 55 0.0029599995 12.147809 1 ACGAAAT 55 0.0031112507 12.067265 12 CTACACT 120 9.640644E-9 11.902566 4 GTCCTAC 795 0.0 11.88586 1 TCCTAGA 210 0.0 11.809452 2 GCTATAC 65 7.748341E-4 11.739573 3 CCGGTTT 65 8.147874E-4 11.669442 12 CTGTAGG 890 0.0 11.58234 1 TCCTACA 915 0.0 11.466932 2 CCTACAC 150 1.6370905E-10 11.446083 3 GACGTGG 270 0.0 11.279448 7 >>END_MODULE