FastQCFastQC Report
Thu 26 May 2016
SRR1926767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926767_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761888
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16670.2187985635683985No Hit
CTGTAGGACGTGGAATATGGCAAGA15830.20777332101306228No Hit
GTATCAACGCAGAGTACTTTTTTTT14340.18821664076609684No Hit
GTCCTAAAGTGTGTATTTCTCATTT13280.17430383468436306No Hit
CTTTAGGACGTGAAATATGGCGAGG12270.1610472930404469No Hit
GTCCTACAGTGTGCATTTCTCATTT11530.15133457936074593No Hit
CTGTAGGACCTGGAATATGGCGAGA9370.12298395564702425No Hit
GGTATCAACGCAGAGTACTTTTTTT8360.10972741400310808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG651.9397157E-716.0547619
CGTGCGC400.00532897214.22848910
TAGGACC3300.014.1451134
CCGTTTC500.00151622713.27905113
TAGACCC655.3058848E-513.1902614
GTCTTAA801.911767E-613.1124931
TTACACT1155.0913513E-912.4256094
TGTAGGA8550.012.1582642
TAGGACA2750.012.1243814
TGGCGAA550.003097814312.07424218
GTATTAG802.7521004E-511.9204471
TACACTG3650.011.7447545
GTAGGAC8450.011.72545053
AAAGGCG657.83136E-411.7246765
TTAGGAC4750.011.4323133
CTAGACA752.0186421E-411.431564
TGGACTG1501.6734703E-1011.431565
GGACCTG6300.011.3408336
GTCCTAC8900.011.2507591
TATACAG1950.011.2361495
Produced by FastQC (version 0.11.5)