FastQCFastQC Report
Thu 26 May 2016
SRR1926766_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926766_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769041
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17610.22898649096732165No Hit
GTCCTAAAGTGTGTATTTCTCATTT14330.18633596908357292No Hit
CTGTAGGACGTGGAATATGGCAAGA13920.18100465384810432No Hit
GTCCTACAGTGTGCATTTCTCATTT13140.17086215169282262No Hit
GTATCAACGCAGAGTACTTTTTTTT10350.13458320167585344No Hit
CTTTAGGACGTGAAATATGGCGAGG9660.12561098823079656No Hit
CTGTAGGACCTGGAATATGGCGAGA7900.10272534234195575No Hit
GTACATGGGAAGCAGTGGTATCAAC7820.10168508571064483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAC402.696297E-416.6750343
CGAGATC400.005171719514.2928863
GTCCTAT1400.013.626521
TCCTATA1051.3060344E-913.6122722
GTCATAA801.9068684E-613.1155261
CTAGGAC1600.013.1018123
GTCTTAG956.987284E-813.05277251
TTAGCCA957.13826E-813.031464
GATATAC2000.012.8770631
TAGGACC3850.012.8622214
ATAGTGC550.003043559812.1027788
TAGACAG1459.458745E-1111.8146975
ACCGTGC657.949583E-411.7037868
GTCCTAC9700.011.7019511
TGTAGGA9700.011.6897152
GTGTTAA907.118195E-611.6582461
CCTAGAC1156.76373E-811.6000243
GTAGGAC9400.011.3532143
CTGTAGG9300.011.2821741
TAGAACA2550.011.2035174
Produced by FastQC (version 0.11.5)