FastQCFastQC Report
Thu 26 May 2016
SRR1926766_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926766_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769041
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17970.23366764580822091No Hit
GTATCAACGCAGAGTACTTTTTTTT17670.22976668344080486No Hit
CTGTAGGACGTGGAATATGGCAAGA16690.21702353970724578No Hit
GTCCTAAAGTGTGTATTTCTCATTT13790.17931423682222405No Hit
GTCCTACAGTGTGCATTTCTCATTT13140.17086215169282262No Hit
CTTTAGGACGTGAAATATGGCGAGG13060.16982189506151169No Hit
GGTATCAACGCAGAGTACTTTTTTT11140.144855735910049No Hit
TATCAACGCAGAGTACTTTTTTTTT10570.13744390741195853No Hit
CTGTAGGACCTGGAATATGGCGAGA10140.1318525280186622No Hit
GTACATGGGAAGCAGTGGTATCAAC8810.11455826152311774No Hit
CCCATGTACTCTGCGTTGATACCAC8200.1066263047093718No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATA402.6821555E-416.6865311
TAGGACC4400.014.0713184
CCTATAC500.001467997213.3361523
TCTATAC957.1106115E-813.0353373
GTCCTAT751.4212374E-512.7135481
ATAGGAC1207.057679E-1012.7010973
ATTGGAC603.9952714E-412.7010973
ACGAAAT550.003098115312.07410912
AGGACCT7350.011.92275
GTTCTAG1052.5857298E-711.8054381
CTGTAGG10900.011.7221241
GGCGAGG3350.011.6107119
CTATAGT1156.7971996E-811.5958964
GGACCTG7800.011.356976
TGTAGGA10800.011.2994542
CTATACA1354.5165507E-911.2891274
TCTTAGG600.005717344611.1229012
TAAGGGT600.005756407511.1127344
TGCACCG600.005756407511.1127345
GTCCTAC8600.011.0873971