FastQCFastQC Report
Thu 26 May 2016
SRR1926765_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926765_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034164
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT27010.26117714405065345No Hit
CTGTAGGACGTGGAATATGGCAAGA20780.20093524818113953No Hit
GTCCTAAAGTGTGTATTTCTCATTT19490.18846140457412944No Hit
GTCCTACAGTGTGCATTTCTCATTT17540.16960559447050952No Hit
CTTTAGGACGTGAAATATGGCGAGG14680.14195040631853362No Hit
CTGTAGGACCTGGAATATGGCGAGA10960.10597932242855099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1151.8189894E-1214.84110218
TAGGACC5650.013.9885754
CCAACGA903.7265636E-813.69595519
TATGTCG500.001520752613.27454116
CTATTAG905.0918607E-712.7279871
CTAGGAC1603.6379788E-1211.9202993
TGTAGGA12200.011.7248842
AGGACCT9200.011.6896755
GTATTAC907.063398E-611.6673211
GGCGAGG3950.011.52220519
GTCCTAC13500.011.4551881
GGACCTG8550.011.3539226
CTTGGAC1104.7230424E-711.2701013
GATATAC3400.011.23057651
TCCTACA14250.011.1758032
GCCTCGA600.00595775811.06211916
TGTCGAG600.00595775811.06211918
TAGGACA3200.011.0102094
ATAGGAC1751.8189894E-1110.8985593
TACAATA1053.307503E-610.8985592
Produced by FastQC (version 0.11.5)