##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926765_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1034164 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.023751551978215 31.0 28.0 31.0 22.0 33.0 2 28.617034628936995 31.0 30.0 31.0 23.0 34.0 3 28.547773854050227 31.0 28.0 33.0 16.0 34.0 4 32.32539519844048 35.0 33.0 37.0 24.0 37.0 5 32.16096769951381 35.0 33.0 37.0 22.0 37.0 6 31.83974108555316 35.0 32.0 37.0 17.0 37.0 7 31.937184044310186 35.0 32.0 37.0 17.0 37.0 8 31.767036949652088 35.0 32.0 37.0 17.0 37.0 9 33.81172135173918 38.0 34.0 39.0 23.0 39.0 10 33.88880873826589 38.0 34.0 39.0 23.0 39.0 11 34.07052072978754 38.0 35.0 39.0 25.0 39.0 12 33.91195206949768 38.0 34.0 39.0 23.0 39.0 13 34.0367098448602 38.0 35.0 39.0 25.0 39.0 14 35.115631563272366 39.0 36.0 41.0 25.0 41.0 15 34.689612092472764 39.0 34.0 41.0 19.0 41.0 16 34.78690807260744 39.0 34.0 41.0 22.0 41.0 17 35.02792110342267 39.0 35.0 41.0 25.0 41.0 18 35.083838733508415 39.0 36.0 41.0 25.0 41.0 19 34.46847792032985 39.0 34.0 41.0 18.0 41.0 20 34.71320312832394 39.0 34.0 41.0 19.0 41.0 21 34.71472513063692 39.0 35.0 41.0 19.0 41.0 22 34.749864624953105 39.0 35.0 41.0 19.0 41.0 23 34.773712873393386 39.0 35.0 41.0 19.0 41.0 24 33.49646767824059 39.0 33.0 41.0 2.0 41.0 25 34.085672098429264 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.3862374173451357 1101 2 -0.5438224505731348 1101 3 -1.3848031171656032 1101 4 -0.4140396195283671 1101 5 -0.49726668580604283 1101 6 -2.2481449405598077 1101 7 -0.6398264514737342 1101 8 -0.19676992172250607 1101 9 -0.47351634091270967 1101 10 -9.16484442005368 1101 11 -4.110747444869141 1101 12 -1.5814015548693945 1101 13 -0.9306875923622826 1101 14 -0.7249621486509383 1101 15 -1.9777736860308792 1101 16 -0.4808485346080076 1101 17 -0.462923522878512 1101 18 -0.25506130386432346 1101 19 -0.867111326566949 1101 20 -0.2529902996411124 1101 21 -0.25914800532247284 1101 22 0.05597479138135242 1101 23 -0.11193921844076016 1101 24 -0.090559057945228 1101 25 -0.014028537011910203 1102 1 -0.3342701480818455 1102 2 -0.25810013737273607 1102 3 -0.2565234431172172 1102 4 -0.10891041267966983 1102 5 -0.2662195805175358 1102 6 -1.0057215495356076 1102 7 0.08627170862644817 1102 8 0.7433543972790915 1102 9 0.5352189018919873 1102 10 0.45251341316726723 1102 11 0.25055454202286853 1102 12 0.24565829806705608 1102 13 0.11798662370910051 1102 14 -0.09725376403088859 1102 15 0.5455818388418407 1102 16 0.3851807496613304 1102 17 0.2984054898834074 1102 18 -0.003705104682772742 1102 19 0.5390232783201867 1102 20 0.21372283725072094 1102 21 0.2783416927370155 1102 22 0.2044739190611935 1102 23 -0.20864678960888483 1102 24 -3.5598200297348583 1102 25 -1.885213251251244 1103 1 0.10590223578061853 1103 2 0.12928113897651627 1103 3 0.4471930205055834 1103 4 0.3483124791903762 1103 5 0.07827458298473289 1103 6 0.8620929445788086 1103 7 0.7455498366987143 1103 8 0.9651051636304118 1103 9 0.8476903129378854 1103 10 0.6119314458188114 1103 11 0.38360000364773583 1103 12 0.3506719694443632 1103 13 0.4214697791531279 1103 14 0.45532246611415417 1103 15 0.6428277937698041 1103 16 0.6338579038906218 1103 17 0.4103974320936672 1103 18 0.25245281657169727 1103 19 -1.059943257486239 1103 20 0.03323566585355309 1103 21 0.11443644941684283 1103 22 0.2742642139229261 1103 23 0.2544904735183806 1103 24 1.7435787813216663 1103 25 0.9218219631999389 1104 1 -0.4478613008665917 1104 2 -0.19559839404310253 1104 3 -1.4278811238844504 1104 4 -0.31952499891394126 1104 5 -0.31280931782930566 1104 6 -1.5838402057527716 1104 7 -0.15219630420799746 1104 8 0.3340732866486107 1104 9 0.34343980245512284 1104 10 0.2758701783267128 1104 11 0.10031694002978497 1104 12 0.14611384393622018 1104 13 0.02190093557096162 1104 14 0.054085400915319326 1104 15 0.36233321103595983 1104 16 -0.04062247701490662 1104 17 0.02527897706069382 1104 18 -0.05177318552367893 1104 19 -1.0752140686486769 1104 20 -0.2558542343008412 1104 21 -0.007242044864312902 1104 22 0.06701320762331164 1104 23 -0.024892540865522506 1104 24 1.3242767749799 1104 25 0.6190829770910682 1105 1 0.12681859667365103 1105 2 0.1732904536386144 1105 3 0.6558107820811578 1105 4 0.5198211163220989 1105 5 0.3303511392443852 1105 6 0.29345040960422253 1105 7 0.5110308747687 1105 8 0.7516962640671707 1105 9 -0.024087382337413032 1105 10 0.3367067138787121 1105 11 0.07659930955078664 1105 12 0.12482284760019269 1105 13 -0.09246568257426446 1105 14 -0.2781472794997413 1105 15 0.36399367274546535 1105 16 -0.2560640123266893 1105 17 -0.14459637030874006 1105 18 -0.10376249451032038 1105 19 -1.1084616536040528 1105 20 -0.4175743546295294 1105 21 -0.20546450796118876 1105 22 -0.04827720038668559 1105 23 -0.2589624336603791 1105 24 -4.985671160695329 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-0.07465545229750248 2215 3 0.14312756919126457 2215 4 0.22514255906884983 2215 5 0.6448343886070091 2215 6 0.873218414438643 2215 7 0.534039445860131 2215 8 -0.7365419958260553 2215 9 -0.23571181334494895 2215 10 -0.3144033801279562 2215 11 -0.4034468892398948 2215 12 -0.5896221411540523 2215 13 -0.2449327766631768 2215 14 -0.3611989027149889 2215 15 -0.3517350130562775 2215 16 -0.11926874949061528 2215 17 -0.4714992992119491 2215 18 -1.184663299413394 2215 19 0.010571389973705436 2215 20 -3.6223183124928013 2215 21 -2.1447020133695176 2215 22 -1.2539944795210687 2215 23 -1.103638309095281 2215 24 -1.119073744537907 2215 25 -0.9064184796804042 2216 1 -0.4331169267246473 2216 2 -1.1948235328242163 2216 3 -0.8213080474205334 2216 4 -0.9962426017313497 2216 5 -0.9179647377540938 2216 6 -2.3454302940951592 2216 7 -10.519534243681523 2216 8 -11.59741025361284 2216 9 -6.459952127348497 2216 10 -3.982268662477921 2216 11 -3.0078103356820307 2216 12 -2.6899513384191813 2216 13 -2.341612315787007 2216 14 -2.424219604158395 2216 15 -3.265700573957769 2216 16 -2.3267089242183943 2216 17 -2.8219092131142034 2216 18 -2.506056373609397 2216 19 -1.9526091620647819 2216 20 -2.637583252984065 2216 21 -5.253213661888132 2216 22 -3.8145699416633825 2216 23 -3.7531128596144008 2216 24 -3.6091978265839977 2216 25 -3.3091343571177347 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49442.0 3 7.0 4 432.0 5 2370.0 6 2951.0 7 2385.0 8 2162.0 9 1798.0 10 1699.0 11 1702.0 12 1677.0 13 1639.0 14 1682.0 15 1746.0 16 1894.0 17 1978.0 18 2252.0 19 2946.0 20 3704.0 21 4929.0 22 6104.0 23 7295.0 24 8593.0 25 9872.0 26 11470.0 27 12404.0 28 13999.0 29 16757.0 30 20019.0 31 24592.0 32 32022.0 33 42198.0 34 57001.0 35 80134.0 36 119864.0 37 199347.0 38 283098.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.373646938876384 18.416943925677465 12.189931064025268 26.019478071420888 2 14.139073617184444 20.044192775004163 42.55258785299028 23.264145754821115 3 19.162480294679273 24.06537712207422 29.624698368186415 27.147444215060094 4 11.319405538412711 16.56047134274432 39.413221245257496 32.70690187358547 5 12.594660151920475 38.060973227255936 36.93578680841269 12.4085798124109 6 31.812924091081417 37.76132134595001 18.4709316270397 11.95482293592888 7 27.60623208856432 31.41860995136333 22.77175678755522 18.203401172517133 8 24.000290937303014 38.502060805896335 21.339863259467585 16.157784997333074 9 25.808323468653526 15.446444459918451 20.421628020332847 38.323604051095174 10 13.398838853711432 30.1209317802373 36.037419012851544 20.442810353199732 11 34.462679638691704 22.506237790371557 24.957931181215063 18.07315138972167 12 22.806087761325095 24.93305183997431 32.9083209140346 19.352539484665996 13 29.001884878324287 22.37241288603014 25.9519409217819 22.67376131386367 14 19.56456985025319 22.884937505560863 28.380715117426103 29.169777526759844 15 22.49415874293053 32.5925703852135 23.05913595815958 21.854134913696395 16 20.906000512575012 28.858360050096955 29.2924047021504 20.943234735177636 17 20.762882749403047 29.383780835377983 28.830975658583807 21.022360756635162 18 20.49625588599093 28.50884361373006 30.846412507265313 20.148487993013696 19 23.216461822359946 27.45049112341337 27.618196519000808 21.71485053522587 20 22.67391480949931 28.49435016228051 28.700343903165532 20.13139112505464 21 22.05185189483396 27.586550382873863 27.558311138447735 22.803286583844447 22 21.745126502522698 29.37193124637939 28.06092004823933 20.822022202858577 23 21.1844441607576 28.797083957122023 28.397955907327244 21.620515974793133 24 22.654203980580647 27.617357497920736 29.04837430610627 20.680064215392353 25 21.805671070192066 28.196843387942206 28.747509719347786 21.24997582251794 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 208.0 1 208.0 2 291.5 3 375.0 4 375.0 5 375.0 6 604.5 7 834.0 8 834.0 9 834.0 10 1318.0 11 1802.0 12 1802.0 13 1802.0 14 3324.5 15 4847.0 16 4847.0 17 4847.0 18 8425.5 19 12004.0 20 12004.0 21 12004.0 22 19679.0 23 27354.0 24 27354.0 25 27354.0 26 39921.5 27 52489.0 28 52489.0 29 52489.0 30 69346.5 31 86204.0 32 86204.0 33 86204.0 34 98287.5 35 110371.0 36 110371.0 37 110371.0 38 118410.5 39 126450.0 40 126450.0 41 126450.0 42 131365.5 43 136281.0 44 136281.0 45 136281.0 46 137837.5 47 139394.0 48 139394.0 49 139394.0 50 130697.0 51 122000.0 52 122000.0 53 122000.0 54 105250.0 55 88500.0 56 88500.0 57 88500.0 58 73943.5 59 59387.0 60 59387.0 61 59387.0 62 47223.0 63 35059.0 64 35059.0 65 35059.0 66 26630.5 67 18202.0 68 18202.0 69 18202.0 70 12977.5 71 7753.0 72 7753.0 73 7753.0 74 5411.5 75 3070.0 76 3070.0 77 3070.0 78 2068.0 79 1066.0 80 1066.0 81 1066.0 82 708.5 83 351.0 84 351.0 85 351.0 86 230.5 87 110.0 88 110.0 89 110.0 90 74.5 91 39.0 92 39.0 93 39.0 94 24.5 95 10.0 96 10.0 97 10.0 98 7.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22597963185722963 2 0.13692219029090164 3 0.14001647707713671 4 0.06681725529026343 5 0.019532685338108847 6 0.018178934869131008 7 0.2948275128509598 8 0.2914431366785152 9 0.016631791476013475 10 0.034617333421004794 11 0.013924290538057795 12 0.014987951620826098 13 0.01402098700012764 14 0.01469786223461656 15 0.01353750468977841 16 0.016341702089803938 17 0.015954916241524553 18 0.013634201151848255 19 0.020112864110527925 20 0.01469786223461656 21 0.013730897613918101 22 0.014794558696686406 23 0.015471433931175326 24 0.013924290538057795 25 0.013924290538057795 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1034164.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.04769080557615 #Duplication Level Percentage of deduplicated Percentage of total 1 93.34367392175807 79.38663918354202 2 5.172176828560062 8.797633914142832 3 0.7440924499836393 1.898500338509166 4 0.25377564292156224 0.863321296527173 5 0.11803729139023027 0.5019399530842 6 0.07751878520879495 0.3955676205636877 7 0.051572074463261536 0.3070260090207532 8 0.0339409174589304 0.2309277322963756 9 0.025647910066368566 0.19631659726203643 >10 0.1503648910893463 2.386424000726384 >50 0.01466368648843709 0.8635637075193643 >100 0.01226441606257375 2.195736745797719 >500 0.001589829184181323 0.9095857369867171 >1k 6.813553646491385E-4 1.0668171640215838 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2701 0.26117714405065345 No Hit CTGTAGGACGTGGAATATGGCAAGA 2078 0.20093524818113953 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1949 0.18846140457412944 No Hit GTCCTACAGTGTGCATTTCTCATTT 1754 0.16960559447050952 No Hit CTTTAGGACGTGAAATATGGCGAGG 1468 0.14195040631853362 No Hit CTGTAGGACCTGGAATATGGCGAGA 1096 0.10597932242855099 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 9.669646206984579E-5 0.0 9 0.0 0.0 0.0 9.669646206984579E-5 0.0 10 0.0 0.0 0.0 9.669646206984579E-5 0.0 11 0.0 0.0 0.0 9.669646206984579E-5 0.0 12 0.0 0.0 0.0 9.669646206984579E-5 0.0 13 0.0 0.0 0.0 9.669646206984579E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 115 1.8189894E-12 14.841102 18 TAGGACC 565 0.0 13.988575 4 CCAACGA 90 3.7265636E-8 13.695955 19 TATGTCG 50 0.0015207526 13.274541 16 CTATTAG 90 5.0918607E-7 12.727987 1 CTAGGAC 160 3.6379788E-12 11.920299 3 TGTAGGA 1220 0.0 11.724884 2 AGGACCT 920 0.0 11.689675 5 GTATTAC 90 7.063398E-6 11.667321 1 GGCGAGG 395 0.0 11.522205 19 GTCCTAC 1350 0.0 11.455188 1 GGACCTG 855 0.0 11.353922 6 CTTGGAC 110 4.7230424E-7 11.270101 3 GATATAC 340 0.0 11.2305765 1 TCCTACA 1425 0.0 11.175803 2 GCCTCGA 60 0.005957758 11.062119 16 TGTCGAG 60 0.005957758 11.062119 18 TAGGACA 320 0.0 11.010209 4 ATAGGAC 175 1.8189894E-11 10.898559 3 TACAATA 105 3.307503E-6 10.898559 2 >>END_MODULE