FastQCFastQC Report
Thu 26 May 2016
SRR1926765_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926765_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034164
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT27260.26359455560239964No Hit
CTGTAGGACGTGGAATATGGCAAGA24640.2382600825401No Hit
GTCCTAAAGTGTGTATTTCTCATTT20090.19426319229832018No Hit
CTTTAGGACGTGAAATATGGCGAGG18930.1830464026982181No Hit
GTCCTACAGTGTGCATTTCTCATTT16970.1640938961325283No Hit
GTATCAACGCAGAGTACTTTTTTTT14550.14069335231162564No Hit
CTGTAGGACCTGGAATATGGCGAGA13550.13102370610464104No Hit
CTGAAGGACCTGGAATATGGCGAGA11190.10820334105615743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG851.7473212E-814.50837318
GTCTAAG801.2251076E-714.307541
CCAACGA801.3099816E-714.22936419
GCGCCAC802.0314237E-613.042953513
CGTGCGC604.1402673E-412.64832410
AGGACGT12700.012.3761115
GACGTGA6800.012.1397477
TGTAGGA13950.011.9633592
GGACGTG12600.011.9445416
TAGGACC5050.011.883744
GTAGGAC13550.011.8826883
TAGGACG13450.011.7568184
CTGTAGG13900.011.7342421
CGCCACC907.5789667E-611.59373814
TTAGGAC7650.011.45763
ACGTGAA7650.011.4077428
GGCGAGG5400.011.24295419
CTTTAGG7350.011.0307571
GGACCTG8550.011.0294036
CGTGAAA8000.011.0277589
Produced by FastQC (version 0.11.5)