##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926765_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1034164 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.845120309738107 31.0 30.0 33.0 26.0 34.0 2 30.19411041188825 31.0 30.0 34.0 26.0 34.0 3 30.261774728186246 31.0 30.0 34.0 26.0 34.0 4 33.87281127558105 35.0 35.0 37.0 30.0 37.0 5 33.851211219883886 37.0 35.0 37.0 30.0 37.0 6 33.7737882966338 37.0 35.0 37.0 29.0 37.0 7 33.77651803775804 37.0 35.0 37.0 29.0 37.0 8 33.80834374431908 37.0 35.0 37.0 29.0 37.0 9 35.37701950561033 39.0 35.0 39.0 30.0 39.0 10 35.26675072812436 39.0 35.0 39.0 29.0 39.0 11 35.30732939843197 39.0 35.0 39.0 30.0 39.0 12 35.20198730568846 39.0 35.0 39.0 29.0 39.0 13 35.21424745011429 39.0 35.0 39.0 29.0 39.0 14 36.32103225407189 40.0 36.0 41.0 29.0 41.0 15 36.31243787252312 40.0 36.0 41.0 29.0 41.0 16 36.288684386615664 40.0 36.0 41.0 29.0 41.0 17 36.26632429672663 40.0 36.0 41.0 29.0 41.0 18 36.233820747966476 40.0 36.0 41.0 29.0 41.0 19 36.30518273697402 40.0 37.0 41.0 29.0 41.0 20 36.269013425336794 40.0 37.0 41.0 29.0 41.0 21 36.2137011151036 40.0 37.0 41.0 29.0 41.0 22 36.1297840574609 40.0 37.0 41.0 29.0 41.0 23 36.060311517322205 40.0 37.0 41.0 29.0 41.0 24 35.994761952649675 40.0 37.0 41.0 28.0 41.0 25 35.87736664590916 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.34849737260204705 1101 2 -0.06755224444190588 1101 3 0.05738254932468578 1101 4 0.02507644804583009 1101 5 -0.06609203240018502 1101 6 -0.1386452654420438 1101 7 0.01094123000056868 1101 8 0.04368517715605691 1101 9 -0.029850309020666543 1101 10 0.11071490476769696 1101 11 0.008630087107782458 1101 12 -0.07020239544230833 1101 13 0.019981722351197106 1101 14 -0.15963344190156903 1101 15 -0.16014289479257826 1101 16 -0.05079291003697506 1101 17 -0.1076998970047427 1101 18 -0.27980069342182645 1101 19 -0.1234916042923544 1101 20 -0.16944491480304436 1101 21 -0.0998637302856693 1101 22 -0.22306140278174524 1101 23 -0.06684884072654995 1101 24 0.05904191087351762 1101 25 0.10850429600766631 1102 1 -0.6952464191150867 1102 2 -0.5982774475373027 1102 3 -0.3350553803688072 1102 4 -0.49423946742652447 1102 5 -0.6236553514267555 1102 6 -0.5804013939817168 1102 7 -0.41934585074166364 1102 8 -0.38941910524020074 1102 9 -0.58260560983733 1102 10 -0.3828452674843206 1102 11 -0.40932632899428256 1102 12 -0.2816561910161326 1102 13 -0.31877952950850386 1102 14 -0.3932118811442038 1102 15 -0.4528129972232975 1102 16 -0.45848886097297736 1102 17 -0.41515306108298944 1102 18 -0.4907135256259565 1102 19 -0.5303286142143264 1102 20 -0.7139278911808304 1102 21 -0.5673203015858022 1102 22 -0.49533277105783213 1102 23 -0.4568432928417039 1102 24 -0.46291370961547784 1102 25 -0.4468916017685416 1103 1 -0.775714534928408 1103 2 -0.6128194909831421 1103 3 -0.46127340927170835 1103 4 -0.6030562227738017 1103 5 -0.6102593206111493 1103 6 -0.6610698834986906 1103 7 -0.5008028933418274 1103 8 -0.5123588596953894 1103 9 -0.6279181816583588 1103 10 -0.6190358822299515 1103 11 -0.6357896706475117 1103 12 -0.5228048276939816 1103 13 -0.6317534367466351 1103 14 -0.5857362015582694 1103 15 -0.6253070875378413 1103 16 -0.7898926862810285 1103 17 -0.8011879510983562 1103 18 -0.6722967277940981 1103 19 -0.7587260753143852 1103 20 -0.6511872698081405 1103 21 -0.6755783311491754 1103 22 -0.8181178495092709 1103 23 -0.8325979854465757 1103 24 -0.8608572238667804 1103 25 -0.7028937759794189 1104 1 -0.6605285905754847 1104 2 -0.49113274033034315 1104 3 -0.3112447075166216 1104 4 -0.40815196843932 1104 5 -0.5561744372888953 1104 6 -0.6887309012138232 1104 7 -0.7574769748096202 1104 8 -0.8125144705734684 1104 9 -0.6907261388886141 1104 10 -0.656725784209435 1104 11 -0.5213102978884123 1104 12 -0.52935864859068 1104 13 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1105 24 0.24037006187433008 1105 25 0.27283959224283194 1106 1 0.17980547957525062 1106 2 0.21489710181530342 1106 3 0.3888948977996236 1106 4 0.2686086386293809 1106 5 0.40720550193096017 1106 6 0.35909339177663924 1106 7 0.4148404526220517 1106 8 0.27949055405211 1106 9 0.20365388318676025 1106 10 0.4185611602994328 1106 11 0.36607238763822636 1106 12 0.21346779073263633 1106 13 0.29149006147209633 1106 14 0.15215003284956907 1106 15 0.08215335788731437 1106 16 0.19567793575219383 1106 17 0.08375808194530521 1106 18 0.22178333693518937 1106 19 0.2953755938234153 1106 20 0.3279079049974598 1106 21 0.13019937929551162 1106 22 0.17672865133614835 1106 23 0.1861963391575614 1106 24 0.0329756575561575 1106 25 0.2578141502953244 1107 1 0.4984667720408922 1107 2 0.584080365942274 1107 3 0.6894469044128826 1107 4 0.6981593714424292 1107 5 0.7127130767060521 1107 6 0.6771985403641239 1107 7 0.6985951637335504 1107 8 0.5091608697333783 1107 9 0.6237290859326308 1107 10 0.5477174208166389 1107 11 0.4627686224884755 1107 12 0.7261254344488819 1107 13 0.6732374830410848 1107 14 0.519779435754721 1107 15 0.4783601224745553 1107 16 0.5087682419018407 1107 17 0.4980446941538972 1107 18 0.4837147713169756 1107 19 0.4874377737323812 1107 20 0.6115094577448019 1107 21 0.5458015621801238 1107 22 0.6587979839396283 1107 23 0.5861314159237168 1107 24 0.6351664984472905 1107 25 0.5513172918319569 1108 1 0.6538385499466415 1108 2 0.6093727808873766 1108 3 0.6760192711528994 1108 4 0.7548186522561195 1108 5 0.6472761990531524 1108 6 0.7154693354810675 1108 7 0.6557036976353814 1108 8 0.6079877776288711 1108 9 0.592954486656474 1108 10 0.5708409882980305 1108 11 0.5992244808936675 1108 12 0.5202794237629149 1108 13 0.5377037757115488 1108 14 0.6305725695810409 1108 15 0.6032562946704445 1108 16 0.6371756816550231 1108 17 0.7965188354054789 1108 18 0.6072391579797269 1108 19 0.6124506449477707 1108 20 0.6253203671962808 1108 21 0.4833176273637747 1108 22 0.5333694201384418 1108 23 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0.5832468368164001 2214 7 0.6850465746124996 2214 8 0.5227331596384985 2214 9 0.47440301413649166 2214 10 0.3783498223739912 2214 11 0.5156648223909457 2214 12 0.4709164689450205 2214 13 0.48385062232485154 2214 14 0.427486427988768 2214 15 0.32573425515154497 2214 16 0.4404744326184584 2214 17 0.38640753735741384 2214 18 0.4468474834982459 2214 19 0.37463784210555673 2214 20 0.5635168064242748 2214 21 0.42138357802517845 2214 22 0.5157012682219104 2214 23 0.4815398558447441 2214 24 0.5498171022068092 2214 25 0.5900637645448086 2215 1 0.2685569081012602 2215 2 0.06412636014756501 2215 3 0.1326084118764541 2215 4 0.16752534341951275 2215 5 0.19185037080683998 2215 6 0.2226684955931688 2215 7 0.12929210176370276 2215 8 -0.10915118487250197 2215 9 -0.15876550555424274 2215 10 -0.004648170205406643 2215 11 -0.27868697527891584 2215 12 -0.20352614896104626 2215 13 -0.27281683625515996 2215 14 -0.3144638791832577 2215 15 -0.14161590182268213 2215 16 -0.24206164594359336 2215 17 -0.040564924786202994 2215 18 -0.10520083490831666 2215 19 -0.3744565863854703 2215 20 -0.12916990043332532 2215 21 -0.23075642162404364 2215 22 -0.2203469991620466 2215 23 -0.31794130804464515 2215 24 -0.214034020210768 2215 25 -0.340318123706119 2216 1 -0.01938782474249834 2216 2 0.16364364300398293 2216 3 -1.7136912842491476 2216 4 -1.27725339803402 2216 5 -0.5571604547186055 2216 6 -0.08232010911688548 2216 7 -0.19539294508360427 2216 8 -0.03343423121334865 2216 9 -0.24759020801056408 2216 10 -0.03414139633860458 2216 11 -0.11857049009279308 2216 12 -0.12940453589445156 2216 13 -0.013874699005221203 2216 14 -0.134272691540815 2216 15 -0.20386583851551876 2216 16 -0.09432645982052179 2216 17 -0.013159252464099325 2216 18 -0.07397457509571126 2216 19 -0.059774071824818975 2216 20 0.004048952440818709 2216 21 -0.02748818916013107 2216 22 -0.11095221567255464 2216 23 -0.3630729869506979 2216 24 -0.4540473147157087 2216 25 -0.3815089315055644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24701.0 3 37.0 4 378.0 5 2608.0 6 3726.0 7 2696.0 8 1992.0 9 1749.0 10 1607.0 11 1446.0 12 1291.0 13 1323.0 14 1289.0 15 1246.0 16 1300.0 17 1460.0 18 1532.0 19 1883.0 20 2358.0 21 3161.0 22 3835.0 23 4725.0 24 5737.0 25 6877.0 26 8154.0 27 8833.0 28 10492.0 29 12722.0 30 15865.0 31 20772.0 32 26874.0 33 35499.0 34 48213.0 35 67716.0 36 100326.0 37 175859.0 38 423882.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.85894097306256 19.218847109254142 10.061510998212949 28.86070091947035 2 14.006193952618876 20.920515459226696 40.65742252960715 24.41586805854728 3 18.62864283068484 24.42235510641858 29.37437075851344 27.574631304383146 4 11.658789128223376 17.404299511489473 38.599970604275526 32.336940756011614 5 12.50059229618484 38.67486178949842 36.15424262965968 12.670303284657065 6 31.579233355488817 38.58673872859853 17.74198399953425 12.092043916378403 7 28.079358493376315 31.553347821587497 21.87890798411128 18.48838570092491 8 24.38410663912493 39.26921563109547 19.87965963520739 16.467018094572207 9 25.951463399302753 15.544864200993166 19.884630076445873 38.619042323258206 10 13.696643142685291 30.45598101577957 35.16200216222071 20.685373679314434 11 34.42579514697079 22.641732028387505 24.6938209119471 18.238651912694596 12 22.915729918784564 25.117607185714437 32.47679524407661 19.48986765142439 13 28.979021519984915 22.51035869864769 25.74198258481562 22.768637196551776 14 19.781872120862843 23.130567298803673 27.748887023721576 29.338673556611912 15 22.00025335330505 32.713616966548656 23.082033285982696 22.204096394163592 16 21.061807590887685 29.079804788616087 28.858659338342868 20.99972828215336 17 20.698691307551428 29.730158331093193 28.462755351676044 21.108395009679334 18 20.539124582510603 28.620578286876857 30.6382583705778 20.202038760034732 19 22.789927699985398 27.629010127030263 27.614408947138898 21.96665322584544 20 22.682115042827277 28.54130042527515 28.539656569524464 20.23692796237311 21 21.9642233713433 27.468431675381783 27.49386621432516 23.073478738949756 22 22.02136218240047 29.462445028061314 27.59784714996848 20.91834563956974 23 21.24469619905547 28.946569402918687 28.123392421318087 21.685341976707758 24 22.28540817145493 27.668419133964633 29.117419821478475 20.928752873101963 25 21.833847463035845 28.43372561913143 28.404908568720927 21.327518349111795 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 258.0 1 258.0 2 263.0 3 268.0 4 268.0 5 268.0 6 482.5 7 697.0 8 697.0 9 697.0 10 1171.5 11 1646.0 12 1646.0 13 1646.0 14 2924.5 15 4203.0 16 4203.0 17 4203.0 18 7696.0 19 11189.0 20 11189.0 21 11189.0 22 18353.0 23 25517.0 24 25517.0 25 25517.0 26 37706.5 27 49896.0 28 49896.0 29 49896.0 30 67158.0 31 84420.0 32 84420.0 33 84420.0 34 96356.0 35 108292.0 36 108292.0 37 108292.0 38 117157.0 39 126022.0 40 126022.0 41 126022.0 42 131330.0 43 136638.0 44 136638.0 45 136638.0 46 139003.5 47 141369.0 48 141369.0 49 141369.0 50 133275.5 51 125182.0 52 125182.0 53 125182.0 54 107688.0 55 90194.0 56 90194.0 57 90194.0 58 75816.5 59 61439.0 60 61439.0 61 61439.0 62 48817.5 63 36196.0 64 36196.0 65 36196.0 66 27351.5 67 18507.0 68 18507.0 69 18507.0 70 13161.5 71 7816.0 72 7816.0 73 7816.0 74 5382.5 75 2949.0 76 2949.0 77 2949.0 78 1989.0 79 1029.0 80 1029.0 81 1029.0 82 675.5 83 322.0 84 322.0 85 322.0 86 206.0 87 90.0 88 90.0 89 90.0 90 56.0 91 22.0 92 22.0 93 22.0 94 12.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11390843231827834 2 0.1180663801872817 3 0.01750205963464209 4 0.0 5 0.0051249124897018265 6 0.3451096731272796 7 0.021563311041575613 8 0.009379556820775042 9 0.009572949744914733 10 0.004061251406933523 11 0.004544733717282752 12 9.669646206984579E-5 13 8.702681586286122E-4 14 0.0 15 0.0033843761724446026 16 2.900893862095374E-4 17 1.9339292413969158E-4 18 5.801787724190748E-4 19 9.669646206984579E-5 20 9.669646206984579E-4 21 0.013247415303568873 22 0.0 23 0.0 24 2.900893862095374E-4 25 0.005221608951771673 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1034164.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.55179667953327 #Duplication Level Percentage of deduplicated Percentage of total 1 91.69188434916367 75.69329793955438 2 6.471954950164006 10.685430183300758 3 0.9755278115272893 2.4159472065729255 4 0.29402127828564045 0.9708793913797065 5 0.15445111446263812 0.6375108499023511 6 0.08445810302245639 0.4183300889189337 7 0.0581073999773226 0.33578091879529764 8 0.03609513948617835 0.23837748927859106 9 0.032708076581339895 0.24300994389492375 >10 0.16740138576061275 2.519285067499542 >50 0.016996076730765006 0.9789762813009452 >100 0.01323255744624697 2.1898649856835752 >500 0.002224940386889932 1.2505219383482784 >1k 9.36817005006287E-4 1.4227877155697248 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2726 0.26359455560239964 No Hit CTGTAGGACGTGGAATATGGCAAGA 2464 0.2382600825401 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2009 0.19426319229832018 No Hit CTTTAGGACGTGAAATATGGCGAGG 1893 0.1830464026982181 No Hit GTCCTACAGTGTGCATTTCTCATTT 1697 0.1640938961325283 No Hit GTATCAACGCAGAGTACTTTTTTTT 1455 0.14069335231162564 No Hit CTGTAGGACCTGGAATATGGCGAGA 1355 0.13102370610464104 No Hit CTGAAGGACCTGGAATATGGCGAGA 1119 0.10820334105615743 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.669646206984579E-5 0.0 10 0.0 0.0 0.0 9.669646206984579E-5 0.0 11 0.0 0.0 0.0 9.669646206984579E-5 0.0 12 0.0 0.0 0.0 9.669646206984579E-5 0.0 13 0.0 0.0 0.0 9.669646206984579E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 85 1.7473212E-8 14.508373 18 GTCTAAG 80 1.2251076E-7 14.30754 1 CCAACGA 80 1.3099816E-7 14.229364 19 GCGCCAC 80 2.0314237E-6 13.0429535 13 CGTGCGC 60 4.1402673E-4 12.648324 10 AGGACGT 1270 0.0 12.376111 5 GACGTGA 680 0.0 12.139747 7 TGTAGGA 1395 0.0 11.963359 2 GGACGTG 1260 0.0 11.944541 6 TAGGACC 505 0.0 11.88374 4 GTAGGAC 1355 0.0 11.882688 3 TAGGACG 1345 0.0 11.756818 4 CTGTAGG 1390 0.0 11.734242 1 CGCCACC 90 7.5789667E-6 11.593738 14 TTAGGAC 765 0.0 11.4576 3 ACGTGAA 765 0.0 11.407742 8 GGCGAGG 540 0.0 11.242954 19 CTTTAGG 735 0.0 11.030757 1 GGACCTG 855 0.0 11.029403 6 CGTGAAA 800 0.0 11.027758 9 >>END_MODULE