FastQCFastQC Report
Thu 26 May 2016
SRR1926764_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926764_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808386
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13870.17157644986429751No Hit
CTGTAGGACGTGGAATATGGCAAGA11380.14077433305376394No Hit
GTCCTAAAGTGTGTATTTCTCATTT11150.1379291576054014No Hit
GTCCTACAGTGTGCATTTCTCATTT9650.11937366555086308No Hit
CTTTAGGACGTGAAATATGGCGAGG8750.10824037031814009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2700.014.4551554
CCTACAC1051.9108484E-812.7025863
ATAAACC951.0028016E-612.0340293
GTATTAT1001.8416995E-611.4422831
TCCTATA1251.7509592E-811.4323262
TAGTACT752.0328713E-411.4230994
CCTATAC855.1641095E-511.2081633
TATACTG1451.1768861E-911.1577265
TTATACA1303.1595846E-810.98374754
CTATACT1158.5683314E-710.760894
TGGCGAG6700.010.7585418
GTCATGG1252.1224514E-710.6794621
GTAGGAC7500.010.6701713
GTCCTAA3400.010.6570271
GGCGAGG3050.010.57288719
GGACGTG7250.010.501396
GTCCTAC6100.010.4731251
AGGACCT5200.010.431975
ATGGCGA7600.010.35807617
TGTAGGA7750.010.3259722
Produced by FastQC (version 0.11.5)