##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926764_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 808386 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.484953970009375 31.0 28.0 31.0 16.0 33.0 2 28.07807285133587 31.0 28.0 31.0 16.0 34.0 3 28.013842397072686 31.0 28.0 31.0 16.0 34.0 4 31.792726494521183 35.0 32.0 37.0 19.0 37.0 5 31.562492423174078 35.0 32.0 37.0 18.0 37.0 6 31.246172001989148 35.0 32.0 37.0 17.0 37.0 7 31.366856922311865 35.0 32.0 37.0 17.0 37.0 8 31.202389699970063 35.0 32.0 37.0 12.0 37.0 9 33.212081851986554 37.0 33.0 39.0 17.0 39.0 10 33.29838344553221 37.0 33.0 39.0 17.0 39.0 11 33.49466591455072 38.0 34.0 39.0 19.0 39.0 12 33.3275390716811 38.0 34.0 39.0 17.0 39.0 13 33.44123228259767 38.0 34.0 39.0 18.0 39.0 14 34.477215092789834 39.0 34.0 41.0 18.0 41.0 15 34.041605124284686 39.0 33.0 41.0 16.0 41.0 16 34.121579295039744 39.0 33.0 41.0 17.0 41.0 17 34.35309493237142 39.0 34.0 41.0 17.0 41.0 18 34.40823566959349 39.0 34.0 41.0 17.0 41.0 19 33.73698827045496 39.0 33.0 41.0 10.0 41.0 20 34.014824601118775 39.0 34.0 41.0 11.0 41.0 21 34.024777767056825 39.0 34.0 41.0 10.0 41.0 22 34.05631715541833 39.0 34.0 41.0 10.0 41.0 23 34.057528210533086 39.0 34.0 41.0 2.0 41.0 24 32.691745032694776 38.0 32.0 40.0 2.0 41.0 25 33.30828836719092 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.165637159087634 1101 2 -0.11296866728397603 1101 3 -0.8790540978765016 1101 4 -0.19833934868614023 1101 5 -0.10596272783861593 1101 6 -2.1797914112947474 1101 7 -0.5442167043198829 1101 8 0.15458580315228332 1101 9 0.03139462448915964 1101 10 -9.10516603902229 1101 11 -3.740804780387016 1101 12 -1.409701384464615 1101 13 -0.5876384149159222 1101 14 -0.37288126929612986 1101 15 -1.939713108679598 1101 16 -0.29730020476207386 1101 17 0.004430108825403067 1101 18 0.16600273081814265 1101 19 -0.568844659540531 1101 20 0.19092463274247962 1101 21 -0.019012151333939187 1101 22 0.3693760864023119 1101 23 0.17945788464945167 1101 24 -0.011767851686620645 1101 25 0.2087174926313935 1102 1 -0.31459134383348086 1102 2 -0.39096315691491057 1102 3 -0.3538083585136569 1102 4 -0.09304576480149507 1102 5 -0.19963768205635546 1102 6 -0.7302020492766985 1102 7 0.1541404137900919 1102 8 0.6827147523875965 1102 9 0.4582126338270953 1102 10 0.39126211995350957 1102 11 0.20111131022061102 1102 12 0.09812564934001244 1102 13 0.006807001083942055 1102 14 -0.029961433617309297 1102 15 0.4397592817317033 1102 16 0.37598044460357016 1102 17 0.1635873853172498 1102 18 -0.12087302190816729 1102 19 0.3777443560171889 1102 20 0.2557298725425241 1102 21 0.13259492965528352 1102 22 0.17999295077134292 1102 23 -0.2975242808925955 1102 24 -3.3662884990724997 1102 25 -1.9693881942674594 1103 1 -0.6787499252988525 1103 2 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1 -0.4308454785148754 2212 2 0.10355597183750476 2212 3 -3.0254129269465366 2212 4 -1.4417076882864848 2212 5 -0.28413683951750457 2212 6 0.5782976074667339 2212 7 0.8188305467240014 2212 8 0.5999849265122918 2212 9 0.6568726850035276 2212 10 0.6061719370913679 2212 11 0.3720805419499982 2212 12 0.41934701862167145 2212 13 0.3570678760761439 2212 14 0.071024648889086 2212 15 -1.0995379784567803 2212 16 0.07517784833570573 2212 17 0.20743061068078816 2212 18 0.17753551581884608 2212 19 0.9362789625203405 2212 20 -0.20213319929171547 2212 21 -0.23945212769976365 2212 22 0.33220726769076947 2212 23 0.5082374366384599 2212 24 1.62842285335838 2212 25 1.1472204308339542 2213 1 0.1543000645817365 2213 2 -0.012142295236905909 2213 3 0.44964087303081257 2213 4 -6.598293938144906 2213 5 -1.3689284353947286 2213 6 -3.525989401254524 2213 7 -1.3579873261947917 2213 8 -3.0402135670883403 2213 9 -0.9956290139431303 2213 10 -0.13020109496208931 2213 11 0.03813134045492461 2213 12 0.21644323563378975 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2214 24 -1.796773861756897 2214 25 -0.9047913892533188 2215 1 1.0803833910715674 2215 2 0.3506317870493696 2215 3 0.7567293732087421 2215 4 0.991965020000233 2215 5 1.2401330466342309 2215 6 1.64931376591856 2215 7 1.0908814427959257 2215 8 -0.42136416521048403 2215 9 0.3059179598599471 2215 10 0.2617690810528046 2215 11 0.27432970622646025 2215 12 -0.010725128221565683 2215 13 0.4289485761175058 2215 14 0.4871268207531756 2215 15 0.3150893871663385 2215 16 0.7519003203143839 2215 17 0.5089725460334193 2215 18 -0.33257792559913923 2215 19 0.8589516504649026 2215 20 -3.3523634046796715 2215 21 -1.6164109077129183 2215 22 -0.39144286080384205 2215 23 -0.19901182934878392 2215 24 -0.16812508292998984 2215 25 -0.08416141175844416 2216 1 -0.798923939382373 2216 2 -1.686826800408177 2216 3 -1.1849096324989148 2216 4 -1.2090861280005747 2216 5 -1.1103478721956073 2216 6 -2.541782592681077 2216 7 -11.119638494869871 2216 8 -12.082161596102267 2216 9 -6.933860424260363 2216 10 -4.581097105547112 2216 11 -3.5882346177321267 2216 12 -3.0510466959431035 2216 13 -3.1255752293487937 2216 14 -2.9374223513746784 2216 15 -4.259358513977531 2216 16 -3.1796841713709156 2216 17 -3.5730061419313977 2216 18 -3.19934772519683 2216 19 -2.879994538003274 2216 20 -3.7201553816445454 2216 21 -6.451588830953998 2216 22 -5.130597334873233 2216 23 -4.987209357314278 2216 24 -5.099370637136946 2216 25 -4.466762494969807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46438.0 3 7.0 4 398.0 5 2061.0 6 2697.0 7 2115.0 8 1798.0 9 1639.0 10 1557.0 11 1535.0 12 1506.0 13 1501.0 14 1495.0 15 1459.0 16 1624.0 17 1784.0 18 2152.0 19 2598.0 20 3202.0 21 4318.0 22 5219.0 23 6337.0 24 7292.0 25 8451.0 26 9640.0 27 10415.0 28 12023.0 29 14058.0 30 16717.0 31 20551.0 32 26518.0 33 34525.0 34 46563.0 35 64785.0 36 95802.0 37 154802.0 38 192804.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63387778324555 18.01196657688222 12.245226818320985 26.108928821551242 2 14.885651229438151 19.722531153940466 41.18871696047518 24.2031006561462 3 19.147260227316355 23.70510551496679 29.548153937041523 27.599480320675333 4 11.833554089350482 16.21145538046371 38.234034388423304 33.7209561417625 5 13.279915071893178 37.44235739835909 36.218422618789845 13.059304910957877 6 32.817802875175225 36.75294996838767 17.95406994799741 12.475177208439687 7 28.1980046732913 31.281409528156335 22.28780429724514 18.232781501307233 8 25.103397130551357 36.78023505717691 21.07044398448826 17.045923827783476 9 26.187727731071643 15.40745689735167 19.93962226800042 38.46519310357627 10 14.276945998487825 29.239197284518013 35.03149954152111 21.45235717547305 11 34.86237170033553 22.383156988310752 24.341675492165926 18.412795819187792 12 23.19598343091016 24.641324735354814 31.72710986177578 20.43558197195924 13 28.9664737424251 21.92172835046062 25.61916804204577 23.492629865068505 14 20.42509072058061 22.03238845358682 27.560745362517338 29.98177546331523 15 23.22908147926366 31.06250780991925 22.7669817028284 22.9414290079887 16 21.9037237839891 28.231199512024318 27.904933825103278 21.9601428788833 17 21.636040601600506 28.710018261812024 27.786782209332827 21.86715892725464 18 21.566308288072054 27.774258601704876 29.320276640231608 21.33915646999146 19 23.744913147680492 26.964121992895418 27.17223596313339 22.118728896290698 20 23.493662867521515 27.533492029713507 27.680475891182454 21.292369211582525 21 22.87323016623199 26.956034384634798 27.0447425853801 23.125992863753112 22 22.85307763686978 28.10986699659759 27.12725023198268 21.909805134549952 23 22.049737086297558 27.95978966903804 27.512279616455302 22.478193628209095 24 23.46396293294897 27.0172530048932 27.75216049191787 21.76662357023996 25 22.528762082920416 27.36601304763637 27.77082877111457 22.33439609832865 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 215.0 1 215.0 2 276.0 3 337.0 4 337.0 5 337.0 6 500.0 7 663.0 8 663.0 9 663.0 10 972.0 11 1281.0 12 1281.0 13 1281.0 14 2273.0 15 3265.0 16 3265.0 17 3265.0 18 5958.5 19 8652.0 20 8652.0 21 8652.0 22 13462.0 23 18272.0 24 18272.0 25 18272.0 26 26634.5 27 34997.0 28 34997.0 29 34997.0 30 46552.0 31 58107.0 32 58107.0 33 58107.0 34 66871.0 35 75635.0 36 75635.0 37 75635.0 38 83498.0 39 91361.0 40 91361.0 41 91361.0 42 96720.0 43 102079.0 44 102079.0 45 102079.0 46 105102.5 47 108126.0 48 108126.0 49 108126.0 50 104306.5 51 100487.0 52 100487.0 53 100487.0 54 89600.0 55 78713.0 56 78713.0 57 78713.0 58 67600.5 59 56488.0 60 56488.0 61 56488.0 62 46211.0 63 35934.0 64 35934.0 65 35934.0 66 27658.0 67 19382.0 68 19382.0 69 19382.0 70 14115.0 71 8848.0 72 8848.0 73 8848.0 74 6267.5 75 3687.0 76 3687.0 77 3687.0 78 2482.0 79 1277.0 80 1277.0 81 1277.0 82 850.5 83 424.0 84 424.0 85 424.0 86 272.0 87 120.0 88 120.0 89 120.0 90 71.5 91 23.0 92 23.0 93 23.0 94 15.0 95 7.0 96 7.0 97 7.0 98 6.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20274967651592188 2 0.11677589666322771 3 0.11937366555086308 4 0.06877902388215532 5 0.02140066750290084 6 0.019421415017083422 7 0.2600242953242634 8 0.25099395585772144 9 0.017442162531266004 10 0.03451321522144124 11 0.01509180020435782 12 0.016576239568720886 13 0.015339206765084995 14 0.016699942849084472 15 0.014720690363267052 16 0.018431788774174715 17 0.017565865811629593 18 0.014349580522176287 19 0.02164807406362802 20 0.016576239568720886 21 0.014596987082903464 22 0.01682364612944806 23 0.01682364612944806 24 0.01496809692399423 25 0.014473283802539877 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 808386.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.85256611040786 #Duplication Level Percentage of deduplicated Percentage of total 1 92.79846629347323 79.66986462404866 2 5.440290680613723 9.341258306344507 3 0.9452170753343767 2.4344793434649277 4 0.30997841015224514 1.06449767801579 5 0.1367738934351025 0.5871194864157508 6 0.08148877955444164 0.4197612500372492 7 0.056543397023852186 0.33980770117681164 8 0.039287420429856744 0.26983406878093313 9 0.026575992971493174 0.20534554741813674 >10 0.14264165118147915 2.2209712479306485 >50 0.011225089673264644 0.6798879343714224 >100 0.01021629314621181 1.7473955269599704 >500 8.633486738032017E-4 0.5701117105315217 >1k 4.3167433690160086E-4 0.44966557450373545 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1387 0.17157644986429751 No Hit CTGTAGGACGTGGAATATGGCAAGA 1138 0.14077433305376394 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1115 0.1379291576054014 No Hit GTCCTACAGTGTGCATTTCTCATTT 965 0.11937366555086308 No Hit CTTTAGGACGTGAAATATGGCGAGG 875 0.10824037031814009 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 270 0.0 14.455155 4 CCTACAC 105 1.9108484E-8 12.702586 3 ATAAACC 95 1.0028016E-6 12.034029 3 GTATTAT 100 1.8416995E-6 11.442283 1 TCCTATA 125 1.7509592E-8 11.432326 2 TAGTACT 75 2.0328713E-4 11.423099 4 CCTATAC 85 5.1641095E-5 11.208163 3 TATACTG 145 1.1768861E-9 11.157726 5 TTATACA 130 3.1595846E-8 10.9837475 4 CTATACT 115 8.5683314E-7 10.76089 4 TGGCGAG 670 0.0 10.75854 18 GTCATGG 125 2.1224514E-7 10.679462 1 GTAGGAC 750 0.0 10.670171 3 GTCCTAA 340 0.0 10.657027 1 GGCGAGG 305 0.0 10.572887 19 GGACGTG 725 0.0 10.50139 6 GTCCTAC 610 0.0 10.473125 1 AGGACCT 520 0.0 10.43197 5 ATGGCGA 760 0.0 10.358076 17 TGTAGGA 775 0.0 10.325972 2 >>END_MODULE