FastQCFastQC Report
Thu 26 May 2016
SRR1926764_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926764_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808386
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA14290.17677198763956822No Hit
GTCCTACAGTGGACATTTCTAAATT13930.17231866954647904No Hit
GTATCAACGCAGAGTACTTTTTTTT13180.16304092351920987No Hit
GTCCTAAAGTGTGTATTTCTCATTT11520.14250617897885418No Hit
CTTTAGGACGTGAAATATGGCGAGG10960.1355787952784932No Hit
GTCCTACAGTGTGCATTTCTCATTT10500.1298884443817681No Hit
GTACATGGGAAGCAGTGGTATCAAC8310.1027974259821422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGA1207.057679E-1012.7031472
CGAAATC604.1325245E-412.65050213
TAGGACC3900.012.2069834
CAATTAT550.002998104812.1272381
GCCTTAA657.8168086E-411.7274391
GTCTAAG657.8168086E-411.7274391
GTAGGAC9050.011.4685313
CTAGGAC1001.8726605E-611.4264463
TGTAGGA9750.011.1396832
CTGTAGG9600.011.1166351
CTTGTAC1201.2315468E-711.1090443
AACTTAT600.005913960711.0726157
ACTGATC951.3762565E-510.9866428
GTGTTAA1053.3367141E-610.8897651
CCCTTAG700.001453217110.8897641
TAGATCC700.001462282310.8816534
CACTGTT1752.0008883E-1110.8466427
ATGCCGG700.00150291210.84597119
TAAGACT1158.54292E-710.7633744
GACGTGG3800.010.7396037
Produced by FastQC (version 0.11.5)