FastQCFastQC Report
Thu 26 May 2016
SRR1926763_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926763_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910459
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT22210.24394289034432082No Hit
CTGTAGGACGTGGAATATGGCAAGA16810.18463214708185652No Hit
GTCCTAAAGTGTGTATTTCTCATTT16260.1785912380458648No Hit
GTCCTACAGTGTGCATTTCTCATTT12640.1388310731180646No Hit
CTTTAGGACGTGAAATATGGCGAGG12470.13696388305239446No Hit
GTATCAACGCAGAGTACTTTTTTTT9170.10071842883644404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACT1151.8189894E-1214.9017614
CGTGCGC400.00533636614.2259410
TAGGACC2900.013.46011454
GGCGAGG3900.012.88845819
TATGTCG550.003107102412.0698316
GTCCTAT959.94025E-712.0432491
AATCCCG951.0595941E-611.97973819
TGTAGGA9450.011.697592
GTAGGAC9500.011.5357133
GGACCTG5150.011.459766
GACTGTA1001.8807368E-611.4227937
TCCTATA1354.491085E-911.2942262
CCTGCAC1354.491085E-911.2942263
GTTCTAA855.1256113E-511.2167521
TTAAGCA600.00577867411.1073314
TGTCGAG600.005946812711.06461918
TTAGGAC6050.011.0258713
TAAACCT951.3261773E-511.0238174
GACGTGA5100.011.012177
GTCCTAA5250.010.8962741
Produced by FastQC (version 0.11.5)