##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926763_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 910459 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.658898423762082 31.0 28.0 31.0 16.0 33.0 2 28.261822882743758 31.0 28.0 31.0 16.0 34.0 3 28.196638179204115 31.0 28.0 33.0 16.0 34.0 4 31.9516145153159 35.0 32.0 37.0 19.0 37.0 5 31.76141265010286 35.0 32.0 37.0 19.0 37.0 6 31.444747100089074 35.0 32.0 37.0 17.0 37.0 7 31.524824291923085 35.0 32.0 37.0 17.0 37.0 8 31.362187643814824 35.0 32.0 37.0 15.0 37.0 9 33.38873249646607 37.0 33.0 39.0 17.0 39.0 10 33.46463377263556 38.0 34.0 39.0 17.0 39.0 11 33.64140614788804 38.0 34.0 39.0 20.0 39.0 12 33.484161285681175 38.0 34.0 39.0 18.0 39.0 13 33.60100015486694 38.0 34.0 39.0 19.0 39.0 14 34.66699214352321 39.0 35.0 41.0 19.0 41.0 15 34.229467773946986 39.0 34.0 41.0 17.0 41.0 16 34.33679385892171 39.0 34.0 41.0 17.0 41.0 17 34.57334487330017 39.0 34.0 41.0 18.0 41.0 18 34.6259007819133 39.0 35.0 41.0 18.0 41.0 19 33.98531619765415 39.0 34.0 41.0 10.0 41.0 20 34.24724012833087 39.0 34.0 41.0 16.0 41.0 21 34.24513240025086 39.0 34.0 41.0 11.0 41.0 22 34.28409406683881 39.0 34.0 41.0 11.0 41.0 23 34.29300276014626 39.0 34.0 41.0 9.0 41.0 24 32.99932671322926 39.0 32.0 41.0 2.0 41.0 25 33.58453153848773 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.3293201823980816 1101 2 -0.28044125897358896 1101 3 -1.3493920387949387 1101 4 -0.5221144390923484 1101 5 -0.46723221413986593 1101 6 -2.3757930775317497 1101 7 -0.7397134441172142 1101 8 0.004345907550586503 1101 9 -0.3613657104257868 1101 10 -9.267500511019062 1101 11 -4.242631937560468 1101 12 -1.6768826719557381 1101 13 -0.9871291968914164 1101 14 -0.650668126351519 1101 15 -1.8725160268838295 1101 16 -0.36020548439444156 1101 17 -0.32425846009995496 1101 18 -0.31652247449367366 1101 19 -0.9920200179802308 1101 20 -0.27669699792532043 1101 21 -0.3817013696690168 1101 22 -0.3113898799529551 1101 23 -0.2452163397196685 1101 24 -0.6398914942280172 1101 25 -0.37159323523043497 1102 1 -0.42001785681668835 1102 2 -0.2694392547411901 1102 3 -0.24168078262268722 1102 4 -0.03844445443200328 1102 5 0.014797994904935052 1102 6 -0.8580225901337784 1102 7 0.15782381333147555 1102 8 0.5411538035544368 1102 9 0.5089458973268961 1102 10 0.42425302437971624 1102 11 0.2184579617800395 1102 12 0.22152625863511588 1102 13 0.2223819495519166 1102 14 0.14306556826616657 1102 15 0.624563280256389 1102 16 0.5218152073456537 1102 17 0.5613491358288769 1102 18 0.05329311373174761 1102 19 0.48486804731967936 1102 20 0.5399907561027391 1102 21 0.2094010701200304 1102 22 0.22248108778897802 1102 23 -0.17898429663427606 1102 24 -3.4798893668304913 1102 25 -1.582150725349237 1103 1 -0.10800380244295837 1103 2 -0.17134872612549046 1103 3 0.3046027590929583 1103 4 -0.011889386345607988 1103 5 0.11890292914148404 1103 6 0.6953218043043989 1103 7 0.5514770134677072 1103 8 0.8159384683070137 1103 9 0.5294747767419636 1103 10 0.5069756496882434 1103 11 0.17556429146642216 1103 12 0.16409791501219217 1103 13 0.2259101315226104 1103 14 0.27156085588762124 1103 15 0.6937645745964289 1103 16 0.4811451669859039 1103 17 0.43838122107532485 1103 18 0.20168380153084797 1103 19 -1.6114157468899037 1103 20 -0.25063285342717023 1103 21 0.06448077123454254 1103 22 0.21504991062295886 1103 23 0.28890717137110045 1103 24 1.6682448993463694 1103 25 0.9033272260383853 1104 1 -0.7274027782682886 1104 2 -0.48489245432427097 1104 3 -1.3919821854070094 1104 4 -0.6594630940476591 1104 5 -0.48237552413854345 1104 6 -1.4771425249847496 1104 7 -0.3756884364123749 1104 8 0.23453566671934212 1104 9 0.11777398930563976 1104 10 0.1413996782998339 1104 11 0.009567243525253843 1104 12 -0.050593762520612984 1104 13 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21 -0.05075774157444357 1108 22 0.06725192159525051 1108 23 0.2675742113952637 1108 24 -0.5029567100209675 1108 25 -0.008013876569833656 1109 1 0.6653352325104969 1109 2 0.7068417353375551 1109 3 -0.7800215914535862 1109 4 0.5130441561172567 1109 5 0.780226693851823 1109 6 0.7618982128517757 1109 7 1.0124256494445127 1109 8 1.4382092910550064 1109 9 0.788093743360136 1109 10 1.008889689875204 1109 11 0.7202078718313913 1109 12 0.24902785977380404 1109 13 0.5001296565208477 1109 14 0.7406463980727764 1109 15 -0.8236663931957295 1109 16 0.43453494846237106 1109 17 0.40587777853807694 1109 18 0.5934571631829257 1109 19 -1.130831236150982 1109 20 0.08487908789032872 1109 21 0.5447560059003891 1109 22 0.6570348739148315 1109 23 0.59207924984306 1109 24 -0.4463203205604884 1109 25 0.009178014008597302 1110 1 0.7095571564792564 1110 2 0.7832589509952754 1110 3 1.1030732853121776 1110 4 0.8540458199037353 1110 5 0.9806179322177471 1110 6 1.065116718601928 1110 7 1.1211945936564938 1110 8 1.384982744157572 1110 9 0.9547501758393508 1110 10 1.0178109772774988 1110 11 0.8871391653449621 1110 12 0.9155687921375204 1110 13 0.9200412916223613 1110 14 0.6232807364033164 1110 15 1.167431325750087 1110 16 0.8268493660932421 1110 17 0.43853360866226154 1110 18 0.6100877177881614 1110 19 -1.3637905830343797 1110 20 0.4705149671970901 1110 21 0.47041486826811507 1110 22 0.647134028570953 1110 23 0.6257277961498389 1110 24 -0.19885913006132228 1110 25 0.07918716629622935 1111 1 0.3720677426539645 1111 2 -0.09735737236913877 1111 3 0.6155638894058342 1111 4 0.488697393486607 1111 5 0.44580703099462937 1111 6 0.6431427775440142 1111 7 0.7299868055021399 1111 8 0.8620778930084434 1111 9 0.2286350146096865 1111 10 0.26894702230667633 1111 11 0.09603006125875879 1111 12 0.33109893285849523 1111 13 0.18446650261245168 1111 14 0.2426848519906386 1111 15 -0.5800879866547177 1111 16 -0.35892579678115766 1111 17 -0.11874715472536934 1111 18 0.20163137803411502 1111 19 0.7688731221484133 1111 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0.15870810986345774 2215 3 0.5219734985568643 2215 4 0.7174071048588466 2215 5 0.9391216207985522 2215 6 1.312636880646508 2215 7 0.843732437016957 2215 8 -0.3447198178071993 2215 9 0.2627546901420672 2215 10 0.056938348667081584 2215 11 0.13767539467296785 2215 12 -0.16798500092784252 2215 13 0.2744274159313633 2215 14 0.36446635548096395 2215 15 0.2827308748542663 2215 16 0.3096022890775316 2215 17 -0.03874834998216414 2215 18 -0.5804099778509197 2215 19 0.74609723636501 2215 20 -3.6565419889087067 2215 21 -1.7674838966229416 2215 22 -0.7620325534748105 2215 23 -0.6037418356334214 2215 24 -0.516139431917459 2215 25 -0.4351814637536293 2216 1 0.1443977663198659 2216 2 -0.7929364752001504 2216 3 -0.26179157955268906 2216 4 -0.4316437157858033 2216 5 -0.17338414678433622 2216 6 -1.367402338917131 2216 7 -9.85910686164496 2216 8 -11.291559182384354 2216 9 -5.863891542802367 2216 10 -3.3462222867557614 2216 11 -2.2437513405455434 2216 12 -1.772615546046822 2216 13 -1.6367847652037 2216 14 -1.6041794160568372 2216 15 -2.4423399840212205 2216 16 -1.5971457829019045 2216 17 -1.982984062855408 2216 18 -1.5477605150600695 2216 19 -0.8954260646698557 2216 20 -1.9086430369609104 2216 21 -4.612901140047896 2216 22 -3.224851418414495 2216 23 -3.3163893209672786 2216 24 -3.5506262818284746 2216 25 -2.8861454740364074 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51810.0 3 7.0 4 448.0 5 2310.0 6 2772.0 7 2347.0 8 1856.0 9 1775.0 10 1612.0 11 1671.0 12 1483.0 13 1523.0 14 1567.0 15 1579.0 16 1747.0 17 1885.0 18 2090.0 19 2719.0 20 3665.0 21 4519.0 22 5482.0 23 6780.0 24 7916.0 25 9070.0 26 10304.0 27 11221.0 28 12698.0 29 15029.0 30 17894.0 31 21537.0 32 28366.0 33 37575.0 34 50840.0 35 70833.0 36 103860.0 37 173441.0 38 238228.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.3465249530771 18.292061360296344 12.235511693572802 26.12590199305376 2 14.463548861066874 19.879090085255015 42.03395683579831 23.6234042178798 3 19.138321845831012 24.016413545684408 29.570557680019444 27.274706928465143 4 11.67465156076378 16.396455212602614 38.658867518901616 33.270025707731996 5 12.929695310457264 37.839773773452656 36.46277127520495 12.767759640885131 6 32.22030455402862 37.28188660465433 18.196779123075117 12.301029718241931 7 27.957595740625884 31.295118971422404 22.44918418179675 18.298101106154963 8 24.503997761517937 37.83310089252863 21.146384829436922 16.516516516516518 9 25.968998209322507 15.583068759818516 20.200599822030828 38.24733320882815 10 13.933360802087623 29.677571762120586 35.35217689877764 21.03689053701415 11 34.61005669565714 22.517216870500793 24.65321833815043 18.219508095691637 12 23.067762740873953 24.693637884648552 32.38468645327421 19.85391292120328 13 29.06648972982711 22.288658978409458 25.638631405263723 23.006219886499704 14 19.884344422485604 22.62050843193755 27.90399532456176 29.59115182101508 15 22.704513197730876 32.06068715204084 22.949814787961877 22.284984862266395 16 21.344593020956466 28.49105977580229 28.696823353122376 21.467523850118866 17 21.0663347530477 29.277351839055584 28.311162426382296 21.34515098151442 18 21.013998420330502 28.15986079574478 30.222438996525387 20.603701787399334 19 23.49759014568989 27.25645749768463 27.38807388535312 21.85787847127236 20 23.062067238134787 28.138927612939657 28.25306743917028 20.545937709755275 21 22.354729848490173 27.40414191303125 27.30845959152107 22.9326686469575 22 22.199299124454843 28.99065133089455 27.523097035010817 21.286952509639782 23 21.499947268508755 28.624301307740986 27.94538335794136 21.9303680658089 24 23.08375938428954 27.243276377625786 28.556253007256966 21.116711230827708 25 22.134620518132895 27.880154717035023 28.371197848637873 21.614026916194206 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 264.0 1 264.0 2 338.0 3 412.0 4 412.0 5 412.0 6 601.0 7 790.0 8 790.0 9 790.0 10 1255.0 11 1720.0 12 1720.0 13 1720.0 14 2929.0 15 4138.0 16 4138.0 17 4138.0 18 7329.5 19 10521.0 20 10521.0 21 10521.0 22 16773.5 23 23026.0 24 23026.0 25 23026.0 26 33223.0 27 43420.0 28 43420.0 29 43420.0 30 57448.0 31 71476.0 32 71476.0 33 71476.0 34 81685.0 35 91894.0 36 91894.0 37 91894.0 38 99463.0 39 107032.0 40 107032.0 41 107032.0 42 111745.5 43 116459.0 44 116459.0 45 116459.0 46 118728.5 47 120998.0 48 120998.0 49 120998.0 50 115046.5 51 109095.0 52 109095.0 53 109095.0 54 95991.5 55 82888.0 56 82888.0 57 82888.0 58 70449.0 59 58010.0 60 58010.0 61 58010.0 62 46764.5 63 35519.0 64 35519.0 65 35519.0 66 27275.0 67 19031.0 68 19031.0 69 19031.0 70 13881.0 71 8731.0 72 8731.0 73 8731.0 74 6012.0 75 3293.0 76 3293.0 77 3293.0 78 2249.5 79 1206.0 80 1206.0 81 1206.0 82 799.0 83 392.0 84 392.0 85 392.0 86 245.0 87 98.0 88 98.0 89 98.0 90 63.0 91 28.0 92 28.0 93 28.0 94 19.0 95 10.0 96 10.0 97 10.0 98 9.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.225600493816855 2 0.13037380046767622 3 0.13289999879181819 4 0.06765818120310745 5 0.02427347085371225 6 0.023065289046513903 7 0.29875041050722767 8 0.29666355102206693 9 0.020758760141862512 10 0.03668479305493163 11 0.017573553559248688 12 0.01977024775415477 13 0.016804710591031558 14 0.01867190065670173 15 0.016585041171540946 16 0.02097842956135312 17 0.01998991717364538 18 0.01559652878383321 19 0.026140660919382424 20 0.018891570076192337 21 0.017024380010522165 22 0.018562065946956426 23 0.020539090722371905 24 0.01768338826899399 25 0.016694875881286254 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 910459.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.84443738547543 #Duplication Level Percentage of deduplicated Percentage of total 1 92.84525044448958 78.77403037876282 2 5.455924707424105 9.258097244378256 3 0.9129694758514129 2.323811445861766 4 0.27552478074448306 0.9350698003208856 5 0.14423055980447114 0.6118580350201261 6 0.07147246976091068 0.36384248912489237 7 0.045926331504128705 0.27276156303526455 8 0.03769632924837896 0.2558659077261084 9 0.025947037291427923 0.19813156027300374 >10 0.15618751161450178 2.4193189531943666 >50 0.014762800760326463 0.8569107088258877 >100 0.01255442702819448 2.292318438219852 >500 9.059892788787536E-4 0.5552076709353596 >1k 6.471351991991096E-4 0.8827758043214706 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2221 0.24394289034432082 No Hit CTGTAGGACGTGGAATATGGCAAGA 1681 0.18463214708185652 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1626 0.1785912380458648 No Hit GTCCTACAGTGTGCATTTCTCATTT 1264 0.1388310731180646 No Hit CTTTAGGACGTGAAATATGGCGAGG 1247 0.13696388305239446 No Hit GTATCAACGCAGAGTACTTTTTTTT 917 0.10071842883644404 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 1.098347097453043E-4 0.0 0.0 0.0 0.0 9 1.098347097453043E-4 0.0 0.0 0.0 0.0 10 1.098347097453043E-4 0.0 0.0 0.0 0.0 11 1.098347097453043E-4 0.0 0.0 0.0 0.0 12 1.098347097453043E-4 0.0 0.0 0.0 1.098347097453043E-4 13 1.098347097453043E-4 0.0 0.0 0.0 1.098347097453043E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACT 115 1.8189894E-12 14.901761 4 CGTGCGC 40 0.005336366 14.22594 10 TAGGACC 290 0.0 13.4601145 4 GGCGAGG 390 0.0 12.888458 19 TATGTCG 55 0.0031071024 12.06983 16 GTCCTAT 95 9.94025E-7 12.043249 1 AATCCCG 95 1.0595941E-6 11.979738 19 TGTAGGA 945 0.0 11.69759 2 GTAGGAC 950 0.0 11.535713 3 GGACCTG 515 0.0 11.45976 6 GACTGTA 100 1.8807368E-6 11.422793 7 TCCTATA 135 4.491085E-9 11.294226 2 CCTGCAC 135 4.491085E-9 11.294226 3 GTTCTAA 85 5.1256113E-5 11.216752 1 TTAAGCA 60 0.005778674 11.107331 4 TGTCGAG 60 0.0059468127 11.064619 18 TTAGGAC 605 0.0 11.025871 3 TAAACCT 95 1.3261773E-5 11.023817 4 GACGTGA 510 0.0 11.01217 7 GTCCTAA 525 0.0 10.896274 1 >>END_MODULE