FastQCFastQC Report
Thu 26 May 2016
SRR1926763_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926763_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910459
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT22480.24690842750744404No Hit
CTGTAGGACGTGGAATATGGCAAGA20590.2261496673655815No Hit
GTATCAACGCAGAGTACTTTTTTTT17140.18825669250345156No Hit
GTCCTAAAGTGTGTATTTCTCATTT15490.17013396539547634No Hit
CTTTAGGACGTGAAATATGGCGAGG15460.16980446126624044No Hit
GTCCTACAGTGTGCATTTCTCATTT12240.13443768472825246No Hit
CTGTAGGACCTGGAATATGGCGAGA10260.11269041219868221No Hit
GGTATCAACGCAGAGTACTTTTTTT9510.10445280896778437No Hit
TATCAACGCAGAGTACTTTTTTTTT9450.10379380070931256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4750.014.6357984
CCAACGA707.356346E-613.55314719
TGTAGGA11200.012.9373092
GGCGAGG4150.012.8020119
CGAAATC604.1378086E-412.6489113
ACGAAAT604.1378086E-412.6489112
CTGTAGG11450.012.4897031
AGGACCT7850.012.3742165
TCCAACG853.9980405E-612.27755618
GTAGGAC11100.012.2687483
TGGCGAG11100.011.70943118
CTAAGAC1552.3646862E-1111.6737153
GGACCTG8100.011.522016
GGACGTG10000.011.4279536
GACGTGG5250.011.387777
AGGACGT10350.011.13351255
GGCGAGA6850.011.07994519
TGAACTG951.3219786E-511.0269715
GTCCTAC9350.010.9103451
TAAACTG1158.5260945E-710.7654625
Produced by FastQC (version 0.11.5)