FastQCFastQC Report
Thu 26 May 2016
SRR1926762_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926762_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633230
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11080.1749759171233201No Hit
GTCCTACAGTGGACATTTCTAAATT8840.1396017244918908No Hit
CTGTAGGACGTGGAATATGGCAAGA7080.11180771599576773No Hit
GGTATCAACGCAGAGTACTTTTTTT6590.10406961135764256No Hit
GTCCTACAGTGTGCATTTCTCATTT6440.10170080381535936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1750.014.6960744
TGTAGGA4550.013.4108982
GTCCTAG603.94506E-412.7194841
GTCTAGG603.94506E-412.7194841
CTACACT1207.057679E-1012.7003094
GTAGGAC4150.012.6358333
CTGTAGG4550.012.579711
CTAAGAC1001.3711906E-712.3946033
ATACTGT1251.36788E-912.1797336
GTCCTAT959.856885E-712.0500391
GTATTGG802.7407677E-511.9245171
TCTACAC1209.444193E-911.9178883
AATACTG1052.653378E-711.78099255
TTGGACA657.835498E-411.7233624
GGACGTG3650.011.4706396
TGGACAG1001.8862593E-611.4185015
GGACCTG2750.011.4185016
AGGACCT2950.011.2894785
GACGTGG2200.011.2454937
TAGGACG3850.011.1333884
Produced by FastQC (version 0.11.5)