##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926762_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 633230 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.56896862119609 31.0 30.0 31.0 22.0 34.0 2 28.5800672741342 31.0 30.0 31.0 22.0 34.0 3 28.245659554980023 31.0 28.0 33.0 16.0 34.0 4 32.16766577704784 35.0 33.0 37.0 22.0 37.0 5 32.20082118661466 35.0 33.0 37.0 22.0 37.0 6 31.699011417652354 35.0 32.0 37.0 17.0 37.0 7 31.578326990193137 35.0 32.0 37.0 17.0 37.0 8 31.256233911848774 35.0 32.0 37.0 17.0 37.0 9 33.47049255404829 37.0 33.0 39.0 18.0 39.0 10 33.64848475277545 38.0 34.0 39.0 19.0 39.0 11 33.75789839394849 38.0 34.0 39.0 23.0 39.0 12 33.673887844858896 38.0 34.0 39.0 21.0 39.0 13 33.75768678047471 38.0 34.0 39.0 23.0 39.0 14 34.81982849833394 39.0 35.0 41.0 23.0 41.0 15 34.3952323800199 39.0 34.0 41.0 17.0 41.0 16 34.48978570187767 39.0 34.0 41.0 18.0 41.0 17 34.71905784628018 39.0 34.0 41.0 22.0 41.0 18 34.769492917265445 39.0 35.0 41.0 22.0 41.0 19 34.14879269775595 39.0 34.0 41.0 15.0 41.0 20 34.42373071395859 39.0 34.0 41.0 18.0 41.0 21 34.413135827424476 39.0 34.0 41.0 18.0 41.0 22 34.44285330764493 39.0 34.0 41.0 18.0 41.0 23 34.444209844764146 39.0 35.0 41.0 16.0 41.0 24 33.142930688691315 39.0 33.0 41.0 2.0 41.0 25 33.733810779653524 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.5803366857464987 1101 2 -0.8193999805451 1101 3 -1.4787630061075987 1101 4 -0.9889208487321781 1101 5 -0.594069423268003 1101 6 -2.663062988799471 1101 7 -1.0018131328352275 1101 8 -0.5793842167743222 1101 9 -0.438467429744378 1101 10 -9.699009971154233 1101 11 -4.653440146037095 1101 12 -2.103971563167068 1101 13 -1.305243946752988 1101 14 -0.9076455229533096 1101 15 -2.391024155140162 1101 16 -0.6069596700398563 1101 17 -0.37158265754209197 1101 18 -0.16201192626541427 1101 19 -1.2283434331549117 1101 20 -0.3694388615036388 1101 21 -0.17144812204494997 1101 22 0.07178180305354687 1101 23 -0.3252597119384859 1101 24 -0.3222777171105662 1101 25 -0.07084485592867651 1102 1 -0.4351260127993726 1102 2 -0.6104560738328608 1102 3 -0.8022773812691568 1102 4 -0.5013831516301863 1102 5 -0.4268384728702088 1102 6 -1.5831031990415916 1102 7 -0.5764795314336162 1102 8 -0.21434874559644967 1102 9 -0.29219405754114547 1102 10 -0.4375974427916489 1102 11 -0.45605840723133895 1102 12 -0.39251911177837684 1102 13 -0.6357583506361522 1102 14 -0.9012946024259776 1102 15 -0.3587054602497375 1102 16 -0.17653430312139307 1102 17 -0.3591990518322348 1102 18 -0.6052976774789016 1102 19 -0.14024968584756436 1102 20 -0.2673944579076988 1102 21 -0.38089178462357154 1102 22 -0.42402426869301024 1102 23 -0.5046973429807906 1102 24 -3.8327639738242425 1102 25 -2.032442925837053 1103 1 -0.08348573109227786 1103 2 -0.26673127999670854 1103 3 0.03746860079308334 1103 4 -0.04315164618264333 1103 5 -0.5168414825614143 1103 6 0.42737413798362667 1103 7 0.17264705617849785 1103 8 0.38159057902884186 1103 9 0.11401192572497365 1103 10 -0.1343048406126357 1103 11 -0.2664093372259231 1103 12 -0.40157587455451704 1103 13 -0.13501917047603484 1103 14 -0.36166780413548594 1103 15 0.23373058381214662 1103 16 0.05709719028737936 1103 17 -0.2313016221085249 1103 18 -0.24543973490381177 1103 19 -1.6627164287597438 1103 20 -0.2134800535255863 1103 21 -0.20176700282618754 1103 22 -0.1310777810026451 1103 23 -0.16296376084631703 1103 24 1.550453988012876 1103 25 0.7928408880436422 1104 1 -0.30090235477635474 1104 2 -0.18779029172204176 1104 3 -1.4105268260180708 1104 4 -0.23385885038123178 1104 5 -0.10615890322586097 1104 6 -1.4776406820956183 1104 7 -0.19913608339646416 1104 8 0.44773899849769805 1104 9 0.2305034532033261 1104 10 0.48290213896726897 1104 11 0.3424451550288907 1104 12 0.30587195805345857 1104 13 0.13506399785962486 1104 14 0.21772123304663182 1104 15 0.4635368888229223 1104 16 0.22152044319375364 1104 17 0.09385353116698525 1104 18 0.03568302574299764 1104 19 -0.9512658674507222 1104 20 -0.2700815847356992 1104 21 0.10050081395152688 1104 22 0.1879449256881358 1104 23 0.16060480014419198 1104 24 1.520785306512657 1104 25 0.9509058872236054 1105 1 0.2681261099599368 1105 2 0.22449037158586194 1105 3 0.5784929167495996 1105 4 0.30259043180951295 1105 5 0.35467770449812264 1105 6 0.5208581750635162 1105 7 0.6096917322325162 1105 8 0.8293229342969966 1105 9 -0.14849018940195435 1105 10 0.2666203273398793 1105 11 0.2272658615960097 1105 12 0.07293513096539073 1105 13 0.05005329262929337 1105 14 -0.030454933187954225 1105 15 0.36490961272878764 1105 16 -0.19536664675866433 1105 17 -0.2619195135670722 1105 18 -0.1840667545641992 1105 19 -0.9766790104493737 1105 20 -0.2429569197535244 1105 21 -0.10854592747278957 1105 22 0.005847533293781737 1105 23 -0.4567834742866381 1105 24 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0.4117624434103391 1107 11 0.026015123789562722 1107 12 0.19079087837371844 1107 13 0.0010005945438464892 1107 14 0.2980663779876309 1107 15 -0.8502887844646381 1107 16 -0.3353087861064026 1107 17 -0.15304629989000773 1107 18 -0.23318803747452677 1107 19 0.5625930759425657 1107 20 0.6061190556058165 1107 21 0.12755005802879538 1107 22 0.06907235019308189 1107 23 0.02798238127788011 1107 24 -3.788535940122525 1107 25 -1.6983451033181787 1108 1 0.832616628627477 1108 2 0.4608482264188076 1108 3 1.1209199050860228 1108 4 0.8210044181258027 1108 5 0.948923645523486 1108 6 -2.7632180912617486 1108 7 -0.6675759314773835 1108 8 0.49918884057271384 1108 9 0.44619635716506423 1108 10 0.8611551559609438 1108 11 0.7557722088098018 1108 12 0.8709528216935567 1108 13 0.9058588941389871 1108 14 0.8031271067325747 1108 15 -0.47579485733913174 1108 16 0.9136790681446101 1108 17 0.5240792061828685 1108 18 0.7455569017967321 1108 19 0.8824311326227274 1108 20 -0.654002401884064 1108 21 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-3.732766706763144 2216 11 -2.677501354356739 2216 12 -2.222629779549216 2216 13 -2.0817810864638986 2216 14 -2.200063454843857 2216 15 -3.2365262336693945 2216 16 -2.2436536787644457 2216 17 -2.4847359536365445 2216 18 -2.351580896631205 2216 19 -1.7057223451363797 2216 20 -2.5368707308380074 2216 21 -4.929803927964684 2216 22 -3.8053999619349987 2216 23 -3.664787684018208 2216 24 -3.9511559033582344 2216 25 -3.591676030808319 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32021.0 3 3.0 4 257.0 5 1749.0 6 2328.0 7 1742.0 8 1420.0 9 1267.0 10 1134.0 11 1113.0 12 1053.0 13 1120.0 14 1089.0 15 1149.0 16 1147.0 17 1347.0 18 1511.0 19 1848.0 20 2356.0 21 3057.0 22 3821.0 23 4674.0 24 5431.0 25 6260.0 26 7167.0 27 7732.0 28 8742.0 29 10546.0 30 12635.0 31 15446.0 32 19949.0 33 26434.0 34 36009.0 35 50583.0 36 73304.0 37 117522.0 38 168264.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.08637589892485 19.455542282102197 13.347995664591261 25.11008615438169 2 13.827474381414529 20.635590741417122 42.65532033311671 22.881614544051633 3 18.52682277378172 24.957109809954954 29.648703648947155 26.86736376731617 4 11.44676271612313 17.332517908530196 40.09535413298694 31.125365242359738 5 12.291082556054874 38.00484926984528 37.03736465087626 12.66670352322358 6 30.734079723010243 38.12479070972307 18.96106726984388 12.180062297422808 7 26.837538882588834 32.55779330617624 22.87753330756999 17.72713450366494 8 23.839885972205725 37.8060735637645 21.1988755592509 17.155164904778875 9 25.420933920482184 16.592220067096708 20.89332760505184 37.09351840736927 10 13.817748659916617 30.215661400069195 35.24676018155306 20.719829758461128 11 33.12373840278238 23.8560728828069 24.784482077817056 18.23570663659366 12 22.364480667172103 25.768258781905484 32.28898155168057 19.57827899924185 13 27.65331715977079 22.962510187449062 26.30620470928653 23.077967943493615 14 20.028114733383877 23.223717462724284 28.387825372757142 28.3603424311347 15 22.22606553104788 31.751048354616312 23.81498416609412 22.207901948241688 16 21.053213501603196 29.794506483865362 28.20742051144351 20.944859503087933 17 20.832234270235265 29.716402233401514 28.39215624333652 21.059207253026702 18 20.469693715094387 28.960444517449645 30.072527861312576 20.49733390614339 19 22.53752278535798 28.144853273351632 28.35019792064851 20.967426020641874 20 22.25795344341217 28.5096711218375 29.008311194366964 20.22406424038337 21 22.01094553294321 27.96022965086435 28.039359694220035 21.989465121972408 22 21.73925404582371 28.992042607901798 28.387419802186624 20.88128354408787 23 21.355012351606973 28.845898697868954 28.42101605393009 21.378072896593988 24 22.08400197116539 28.116747324395696 28.843772507297103 20.95547819714181 25 21.569401689692373 28.362761062841457 28.75446391200585 21.313373335460323 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 293.0 1 293.0 2 362.5 3 432.0 4 432.0 5 432.0 6 661.0 7 890.0 8 890.0 9 890.0 10 1346.0 11 1802.0 12 1802.0 13 1802.0 14 3035.5 15 4269.0 16 4269.0 17 4269.0 18 7062.5 19 9856.0 20 9856.0 21 9856.0 22 14896.5 23 19937.0 24 19937.0 25 19937.0 26 27354.0 27 34771.0 28 34771.0 29 34771.0 30 43753.5 31 52736.0 32 52736.0 33 52736.0 34 59734.5 35 66733.0 36 66733.0 37 66733.0 38 71773.0 39 76813.0 40 76813.0 41 76813.0 42 79006.5 43 81200.0 44 81200.0 45 81200.0 46 81623.0 47 82046.0 48 82046.0 49 82046.0 50 77336.5 51 72627.0 52 72627.0 53 72627.0 54 63170.5 55 53714.0 56 53714.0 57 53714.0 58 45036.0 59 36358.0 60 36358.0 61 36358.0 62 28717.0 63 21076.0 64 21076.0 65 21076.0 66 15907.5 67 10739.0 68 10739.0 69 10739.0 70 7615.0 71 4491.0 72 4491.0 73 4491.0 74 3076.0 75 1661.0 76 1661.0 77 1661.0 78 1105.0 79 549.0 80 549.0 81 549.0 82 358.0 83 167.0 84 167.0 85 167.0 86 111.0 87 55.0 88 55.0 89 55.0 90 31.0 91 7.0 92 7.0 93 7.0 94 6.0 95 5.0 96 5.0 97 5.0 98 4.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1934526159531292 2 0.12302007169590828 3 0.12649432275792366 4 0.06806373671493769 5 0.02289847290873774 6 0.021793029389005573 7 0.2908895661923787 8 0.28504650758808014 9 0.01800293732135243 10 0.0390063641962636 11 0.016423732293163622 12 0.017371255310076907 13 0.015476209276250335 14 0.017371255310076907 15 0.015002447767793694 16 0.018950460338265716 17 0.018160857824171314 18 0.014844527264974811 19 0.023372234417194385 20 0.017055414304439145 21 0.015002447767793694 22 0.017055414304439145 23 0.01800293732135243 24 0.01610789128752586 25 0.015318288773431455 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 633230.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.83603837261744 #Duplication Level Percentage of deduplicated Percentage of total 1 93.46729873779867 82.09797238518182 2 5.146883969364636 9.041637956650435 3 0.7435859317227632 1.9594092729641732 4 0.23017221326008092 0.8086966142489099 5 0.12050038835989178 0.5292138367947379 6 0.0625662779458489 0.32973443942900593 7 0.042811066676664095 0.2632248146768905 8 0.029471551776943224 0.20709314822241331 9 0.02628210070407076 0.2077664045360179 >10 0.11044097033058467 1.7897086808409153 >50 0.009363294302161452 0.5518197328175395 >100 0.009183023486527511 1.316814697284951 >500 0.0012604149872609957 0.7216701249541477 >1k 1.8005928389442795E-4 0.17523789139802665 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1108 0.1749759171233201 No Hit GTCCTACAGTGGACATTTCTAAATT 884 0.1396017244918908 No Hit CTGTAGGACGTGGAATATGGCAAGA 708 0.11180771599576773 No Hit GGTATCAACGCAGAGTACTTTTTTT 659 0.10406961135764256 No Hit GTCCTACAGTGTGCATTTCTCATTT 644 0.10170080381535936 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.5792050281888097E-4 0.0 6 0.0 0.0 0.0 1.5792050281888097E-4 0.0 7 0.0 0.0 0.0 1.5792050281888097E-4 0.0 8 0.0 0.0 0.0 1.5792050281888097E-4 0.0 9 0.0 0.0 0.0 1.5792050281888097E-4 0.0 10 0.0 0.0 0.0 1.5792050281888097E-4 0.0 11 0.0 0.0 0.0 1.5792050281888097E-4 0.0 12 0.0 0.0 0.0 1.5792050281888097E-4 0.0 13 0.0 0.0 0.0 1.5792050281888097E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 175 0.0 14.696074 4 TGTAGGA 455 0.0 13.410898 2 GTCCTAG 60 3.94506E-4 12.719484 1 GTCTAGG 60 3.94506E-4 12.719484 1 CTACACT 120 7.057679E-10 12.700309 4 GTAGGAC 415 0.0 12.635833 3 CTGTAGG 455 0.0 12.57971 1 CTAAGAC 100 1.3711906E-7 12.394603 3 ATACTGT 125 1.36788E-9 12.179733 6 GTCCTAT 95 9.856885E-7 12.050039 1 GTATTGG 80 2.7407677E-5 11.924517 1 TCTACAC 120 9.444193E-9 11.917888 3 AATACTG 105 2.653378E-7 11.7809925 5 TTGGACA 65 7.835498E-4 11.723362 4 GGACGTG 365 0.0 11.470639 6 TGGACAG 100 1.8862593E-6 11.418501 5 GGACCTG 275 0.0 11.418501 6 AGGACCT 295 0.0 11.289478 5 GACGTGG 220 0.0 11.245493 7 TAGGACG 385 0.0 11.133388 4 >>END_MODULE