FastQCFastQC Report
Thu 26 May 2016
SRR1926762_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926762_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633230
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18540.29278461222620533No Hit
GGTATCAACGCAGAGTACTTTTTTT12380.19550558248977462No Hit
TATCAACGCAGAGTACTTTTTTTTT10050.1587101053329754No Hit
GTCCTACAGTGGACATTTCTAAATT8790.13881212197779638No Hit
CTGTAGGACGTGGAATATGGCAAGA8620.13612747342987538No Hit
CTTTAGGACGTGAAATATGGCGAGG6760.10675425990556354No Hit
GTCCTACAGTGTGCATTTCTCATTT6650.10501713437455584No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6490.10249040632945375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTA400.00517163414.2923645
TAGGACC2200.014.2923644
TGTAGGA5450.013.475822
GTAGGAC5400.012.8817943
TAAGACT751.4309358E-512.7043244
ATAGGCA701.05915526E-412.2505974
CTGTAGG6000.012.2415081
GGCGAGG2250.012.22652719
GGACGTG5150.012.2109526
AGGACGT5100.011.957015
GTCTAGG802.7430577E-511.9235471
TTAGACA802.774449E-511.9103044
GTCCTAT1052.570032E-711.8099891
TAGGACG5300.011.6855814
GACGTGG2200.011.6411117
GGACCTG3450.011.59966
TGTACTG1406.4392225E-1011.5700085
GGTATCA4750.011.4466051
TGTAGGG751.994572E-411.4456962
TATAGTG1251.7444108E-811.4338915
Produced by FastQC (version 0.11.5)