##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926762_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 633230 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.694772831356694 31.0 30.0 33.0 25.0 34.0 2 29.983963172938743 31.0 30.0 33.0 26.0 34.0 3 30.014381820191716 31.0 30.0 34.0 26.0 34.0 4 33.643426559070164 35.0 35.0 37.0 28.0 37.0 5 33.59793124141307 35.0 35.0 37.0 28.0 37.0 6 33.5635014133885 37.0 35.0 37.0 28.0 37.0 7 33.53733398607141 37.0 35.0 37.0 28.0 37.0 8 33.59362632850623 37.0 35.0 37.0 28.0 37.0 9 35.10132337381362 39.0 35.0 39.0 29.0 39.0 10 34.98008148697946 39.0 35.0 39.0 28.0 39.0 11 35.033395448731106 39.0 35.0 39.0 29.0 39.0 12 34.92801509720007 39.0 35.0 39.0 27.0 39.0 13 34.926197432212625 39.0 35.0 39.0 27.0 39.0 14 35.996517852912845 40.0 36.0 41.0 27.0 41.0 15 35.98354784201633 40.0 36.0 41.0 27.0 41.0 16 35.955177423684916 40.0 36.0 41.0 27.0 41.0 17 35.93683021966742 40.0 36.0 41.0 27.0 41.0 18 35.91251204143834 40.0 36.0 41.0 27.0 41.0 19 36.001869778753374 40.0 36.0 41.0 27.0 41.0 20 35.95709142017908 40.0 36.0 41.0 27.0 41.0 21 35.903799567297824 40.0 36.0 41.0 27.0 41.0 22 35.81314056503956 40.0 36.0 41.0 27.0 41.0 23 35.74361132605846 40.0 36.0 41.0 27.0 41.0 24 35.674543214945594 40.0 36.0 41.0 27.0 41.0 25 35.55479367686307 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.055214411454894474 1101 2 -0.23657398354893644 1101 3 0.04951519719948294 1101 4 -0.035661861913325765 1101 5 -0.06959503354747909 1101 6 -0.19918306055325985 1101 7 0.017767559464665794 1101 8 -0.2562601177811601 1101 9 -0.1274913787722909 1101 10 -0.09974379335574213 1101 11 -0.05351691780759893 1101 12 -0.006192453301736123 1101 13 -0.18129998823470572 1101 14 -0.11060653328311787 1101 15 -0.12421933181092015 1101 16 -0.17140402327055426 1101 17 -0.0055698770395338215 1101 18 -0.17619652198732183 1101 19 -0.34697772419067974 1101 20 -0.08798678828597417 1101 21 -0.19581098050775836 1101 22 -0.21623096094751304 1101 23 -0.12446309701849145 1101 24 -0.11700355685537289 1101 25 -0.028776565474245785 1102 1 -0.7699951220273142 1102 2 -0.8360581435857029 1102 3 -0.5723603233795451 1102 4 -0.7942556523030788 1102 5 -1.0223859115668432 1102 6 -1.1070165901508702 1102 7 -1.0357809944026997 1102 8 -0.9073845684805306 1102 9 -0.8467262353078908 1102 10 -1.07554756737418 1102 11 -0.9088393177846186 1102 12 -1.0230956902262278 1102 13 -1.0633874742329255 1102 14 -1.2136837656695931 1102 15 -1.1078296371215401 1102 16 -1.235275120722946 1102 17 -1.086639469480012 1102 18 -1.210532622168266 1102 19 -1.301574616513392 1102 20 -1.2639008532542348 1102 21 -1.3239104721354096 1102 22 -1.3060215804724606 1102 23 -1.3793282490993732 1102 24 -1.2052386144421874 1102 25 -1.2797987672722186 1103 1 -0.6160024656738585 1103 2 -0.5329605693968134 1103 3 -0.5417361134234859 1103 4 -0.47219220801245854 1103 5 -0.588961484913014 1103 6 -0.5175348589764894 1103 7 -0.43016491061289486 1103 8 -0.7510732460222158 1103 9 -0.6090870370896297 1103 10 -0.3703865488946789 1103 11 -0.2919946780446594 1103 12 -0.5263293024576114 1103 13 -0.46220406365085154 1103 14 -0.6165152831558984 1103 15 -0.37659265403600983 1103 16 -0.4987615470156115 1103 17 -0.4291182511570071 1103 18 -0.690444739185061 1103 19 -0.32930591128255315 1103 20 -0.3645027914953758 1103 21 -0.3080156909399605 1103 22 -0.19948693892668246 1103 23 -0.3696093542579817 1103 24 -0.3313374527013835 1103 25 -0.4230143213563693 1104 1 -0.2895186156383218 1104 2 -0.3466387775814894 1104 3 0.02197121199788299 1104 4 0.026584868046519716 1104 5 -0.2895340842859824 1104 6 -0.24687409974157504 1104 7 -0.10590704361092662 1104 8 -0.33611340403967205 1104 9 -0.2985938980613625 1104 10 -0.10577860951557483 1104 11 -0.08157807575283726 1104 12 -0.2726373556910531 1104 13 -0.09830397023800685 1104 14 -0.10162746176697368 1104 15 -0.2338350021450495 1104 16 -0.12446719433337705 1104 17 -0.058395216689334006 1104 18 -0.06540700562322854 1104 19 -0.14260262424364356 1104 20 -0.08551093719089664 1104 21 -0.11936808556527723 1104 22 0.012409294372503155 1104 23 -0.054759325853822816 1104 24 -0.03472432213494159 1104 25 0.08856720809482255 1105 1 0.13136677374936312 1105 2 -0.007284071772616585 1105 3 0.23475327892913 1105 4 0.1725693657334446 1105 5 0.17700029188507216 1105 6 0.007350466844521009 1105 7 -0.08640533537611361 1105 8 -0.03625409509132993 1105 9 -0.15247572196086878 1105 10 0.16350121446721033 1105 11 0.10399824256833767 1105 12 0.243741537144885 1105 13 0.23571236482584368 1105 14 0.24857163141126648 1105 15 0.13230688543797697 1105 16 0.09083065651219613 1105 17 0.0879651145701601 1105 18 0.17988083029857194 1105 19 0.1585452569535022 1105 20 0.36951942534057736 1105 21 0.23368317901060465 1105 22 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0.6486178942467618 1107 10 0.7794989366196958 1107 11 0.7323599381514754 1107 12 0.7293761338245446 1107 13 0.7078544559563724 1107 14 0.5360459640601363 1107 15 0.41863605934415205 1107 16 0.45307699657654155 1107 17 0.5124906322816685 1107 18 0.628444289966545 1107 19 0.4927300120017577 1107 20 0.5263770764973756 1107 21 0.5654570735479254 1107 22 0.541590849771552 1107 23 0.5747564428800587 1107 24 0.6647537260764693 1107 25 0.5775740306268986 1108 1 0.5461324630476376 1108 2 0.4651323586574314 1108 3 0.5927868480834171 1108 4 0.5085477178687157 1108 5 0.5371599906106397 1108 6 0.4936979300373352 1108 7 0.48753118417263863 1108 8 0.35423048787136935 1108 9 0.20732234768805569 1108 10 0.37362849127556075 1108 11 0.2930886830907511 1108 12 0.5593356479945868 1108 13 0.34348198632074656 1108 14 0.258718028837464 1108 15 0.4894862617733011 1108 16 0.5077328670218861 1108 17 0.5276728185164856 1108 18 0.60212850049286 1108 19 0.3115559229329321 1108 20 0.5182799105054698 1108 21 0.44804816666533753 1108 22 0.4919957080711441 1108 23 0.36018154409463676 1108 24 0.5058468434853793 1108 25 0.2719060144325667 1109 1 0.49423589470369933 1109 2 0.3338024312748722 1109 3 0.3776857941665739 1109 4 0.35427180372438016 1109 5 0.4590641098831796 1109 6 0.44466509153080835 1109 7 0.35461475849305657 1109 8 0.25790393430797565 1109 9 0.23204360596194817 1109 10 0.23653093970433048 1109 11 0.151753775308471 1109 12 0.14265291552237613 1109 13 0.18442247857702654 1109 14 0.17295072596489547 1109 15 0.24372763443572865 1109 16 0.3247152267674025 1109 17 0.1078532375126997 1109 18 0.06679577766013267 1109 19 0.08271860527982966 1109 20 0.1762911244377392 1109 21 0.23275833953340452 1109 22 0.0759797065288268 1109 23 0.3629046981335762 1109 24 0.15273650353292823 1109 25 0.12630161882817248 1110 1 0.5369262759409423 1110 2 0.5047281461936102 1110 3 0.5011952261879706 1110 4 0.8616059520729848 1110 5 0.8210179309596342 1110 6 0.7855036868559466 1110 7 0.6709959719954028 1110 8 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6 0.06067816342623189 2210 7 0.003996969004376183 2210 8 0.07050877850554116 2210 9 0.005118231292307485 2210 10 0.15364269966254795 2210 11 0.05071660902952857 2210 12 -0.018941665812981512 2210 13 0.22259565745309828 2210 14 0.19457039077997962 2210 15 0.06820657855575263 2210 16 0.08264678922289193 2210 17 0.1307300436023624 2210 18 0.3177700374205372 2210 19 0.09096667852042373 2210 20 0.013251473549132697 2210 21 0.20925543554695025 2210 22 0.14104047659957075 2210 23 0.16582332321072357 2210 24 -0.04071488832324377 2210 25 0.10358405722725905 2211 1 0.10090851959271419 2211 2 0.19723257598568722 2211 3 0.2617526119306426 2211 4 0.3437874742385816 2211 5 0.11844850479260316 2211 6 0.24575003319475286 2211 7 0.21867090597658034 2211 8 0.171648930180055 2211 9 0.28791557038395865 2211 10 0.33476567029921256 2211 11 0.1601429913154533 2211 12 0.06333456731296394 2211 13 0.050337163063836954 2211 14 0.16754743609153877 2211 15 0.19652916721763347 2211 16 0.22594016718517906 2211 17 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-0.43645731186379066 2215 24 -0.535941914427255 2215 25 -0.4861687836974795 2216 1 -0.21263006895195602 2216 2 0.021962885800231646 2216 3 -1.7392533445344043 2216 4 -1.1777754394997402 2216 5 -0.2582502996317828 2216 6 -0.04473447516680551 2216 7 -0.0769162801062322 2216 8 -0.062234125558497055 2216 9 -0.15814684812126245 2216 10 -0.19328402249225007 2216 11 -0.21788797647116098 2216 12 -0.12002560063803003 2216 13 -0.04024812318271387 2216 14 -0.22207845562417106 2216 15 -0.1668042663468654 2216 16 -0.33046947942705174 2216 17 -0.265635178280732 2216 18 -0.4153166819941063 2216 19 -0.38595816440668784 2216 20 -0.2568592712822948 2216 21 -0.1988915256122823 2216 22 -0.13268523577121272 2216 23 -0.3497395517669375 2216 24 -0.3400786355217633 2216 25 -0.3577811507699238 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17078.0 3 19.0 4 257.0 5 1669.0 6 2431.0 7 1776.0 8 1393.0 9 1151.0 10 1092.0 11 985.0 12 895.0 13 848.0 14 859.0 15 836.0 16 844.0 17 907.0 18 1013.0 19 1202.0 20 1570.0 21 2003.0 22 2571.0 23 3057.0 24 3701.0 25 4392.0 26 5306.0 27 5673.0 28 6670.0 29 8167.0 30 10271.0 31 13461.0 32 17104.0 33 22829.0 34 30802.0 35 43317.0 36 64099.0 37 111051.0 38 241931.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.543548542637936 20.44053643444677 10.9345234425268 28.08139158038849 2 13.818079499720204 21.425658308647087 40.40872201654743 24.347540175085282 3 18.296427330448243 25.222383675016584 29.692327131440123 26.788861863095047 4 12.010801762392811 18.267770004579695 38.615826792792504 31.105601440234988 5 12.65792598956425 38.79783578223901 35.6176801303837 12.92655809781304 6 30.6188032129405 38.98423795662308 17.97113804583622 12.425820784600198 7 27.27679093232413 32.60007013043629 21.914971584537188 18.208167352702393 8 24.348714644227233 38.67138577098446 20.013550841715745 16.966348743072565 9 25.60284158390822 16.77974881863897 20.447179895383215 37.17022970206959 10 14.184754456671426 30.56720698411896 34.22635216232265 21.021686396886963 11 33.20162222601247 23.95041724310017 24.515322076297686 18.332638454589674 12 22.620589238563287 26.04370649248923 31.566870491326927 19.768833777620557 13 28.004826103916 22.965527152361805 26.04122086531917 22.988425878403028 14 20.202959750737882 23.415857454412226 27.9759770951741 28.40520569967579 15 22.10405792752742 31.78528280730569 23.798295970499286 22.312363294667602 16 21.28886663939876 29.9812230446462 27.72214527899334 21.007765036961704 17 20.72852001408653 29.860761272778095 28.17132506565555 21.239393647479822 18 20.513812582217753 28.947439070033337 29.9469226675426 20.59182568020631 19 22.149806783959676 28.34961759489853 28.344248289323453 21.156327331818346 20 22.221976564227283 28.356495372856195 29.03793310381858 20.383594959097945 21 21.903493850500915 27.98570934436405 28.10005986028389 22.010736944851146 22 21.84309018839916 28.907979723007436 28.227500276360878 21.021429812232522 23 21.205440685755075 28.801902629222603 28.432368069055585 21.560288615966737 24 21.727176539330102 27.99867346777632 29.188446536013767 21.085703456879806 25 21.5638493100061 28.45415465086529 28.718528602608362 21.263467436520244 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 339.0 1 339.0 2 339.0 3 339.0 4 339.0 5 339.0 6 511.5 7 684.0 8 684.0 9 684.0 10 1127.5 11 1571.0 12 1571.0 13 1571.0 14 2685.0 15 3799.0 16 3799.0 17 3799.0 18 6436.5 19 9074.0 20 9074.0 21 9074.0 22 13990.5 23 18907.0 24 18907.0 25 18907.0 26 26218.5 27 33530.0 28 33530.0 29 33530.0 30 42565.5 31 51601.0 32 51601.0 33 51601.0 34 58741.5 35 65882.0 36 65882.0 37 65882.0 38 70779.5 39 75677.0 40 75677.0 41 75677.0 42 78567.5 43 81458.0 44 81458.0 45 81458.0 46 82288.5 47 83119.0 48 83119.0 49 83119.0 50 78779.5 51 74440.0 52 74440.0 53 74440.0 54 64862.5 55 55285.0 56 55285.0 57 55285.0 58 46318.0 59 37351.0 60 37351.0 61 37351.0 62 29645.5 63 21940.0 64 21940.0 65 21940.0 66 16476.5 67 11013.0 68 11013.0 69 11013.0 70 7917.0 71 4821.0 72 4821.0 73 4821.0 74 3238.0 75 1655.0 76 1655.0 77 1655.0 78 1099.5 79 544.0 80 544.0 81 544.0 82 348.5 83 153.0 84 153.0 85 153.0 86 96.5 87 40.0 88 40.0 89 40.0 90 24.0 91 8.0 92 8.0 93 8.0 94 4.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10785970342529569 2 0.09854239375898172 3 0.014212845253699287 4 0.0 5 0.0034742510620153814 6 0.3406345245803263 7 0.01958214234954124 8 0.009317309666313977 9 0.00979107117477062 10 0.0034742510620153814 11 0.0041059330732909054 12 9.475230169132858E-4 13 0.0011054435197321668 14 1.5792050281888097E-4 15 0.0039480125704720245 16 0.0011054435197321668 17 1.5792050281888097E-4 18 4.737615084566429E-4 19 1.5792050281888097E-4 20 0.0015792050281888096 21 0.014054924750880408 22 0.0 23 1.5792050281888097E-4 24 0.0 25 0.0056851381014797146 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 633230.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.49075245937189 #Duplication Level Percentage of deduplicated Percentage of total 1 91.96033259788476 78.61758030207272 2 6.359381063675984 10.873365446190812 3 0.941546480538524 2.4148055129013533 4 0.28043916643136063 0.9589982142918417 5 0.12643735981294596 0.5404612514692549 6 0.07150299195419164 0.36677067511561545 7 0.04674105331945488 0.27971494733166735 8 0.03702898256685828 0.2532508465956542 9 0.02458529698259256 0.18916339846310667 >10 0.1303533593586282 2.050679841279778 >50 0.00925294033254593 0.5280970562926096 >100 0.01054815383795074 1.4966211398056741 >500 0.0012953873129791286 0.7823246865404827 >1k 5.551659912767694E-4 0.648166681649415 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1854 0.29278461222620533 No Hit GGTATCAACGCAGAGTACTTTTTTT 1238 0.19550558248977462 No Hit TATCAACGCAGAGTACTTTTTTTTT 1005 0.1587101053329754 No Hit GTCCTACAGTGGACATTTCTAAATT 879 0.13881212197779638 No Hit CTGTAGGACGTGGAATATGGCAAGA 862 0.13612747342987538 No Hit CTTTAGGACGTGAAATATGGCGAGG 676 0.10675425990556354 No Hit GTCCTACAGTGTGCATTTCTCATTT 665 0.10501713437455584 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 649 0.10249040632945375 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACTA 40 0.005171634 14.292364 5 TAGGACC 220 0.0 14.292364 4 TGTAGGA 545 0.0 13.47582 2 GTAGGAC 540 0.0 12.881794 3 TAAGACT 75 1.4309358E-5 12.704324 4 ATAGGCA 70 1.05915526E-4 12.250597 4 CTGTAGG 600 0.0 12.241508 1 GGCGAGG 225 0.0 12.226527 19 GGACGTG 515 0.0 12.210952 6 AGGACGT 510 0.0 11.95701 5 GTCTAGG 80 2.7430577E-5 11.923547 1 TTAGACA 80 2.774449E-5 11.910304 4 GTCCTAT 105 2.570032E-7 11.809989 1 TAGGACG 530 0.0 11.685581 4 GACGTGG 220 0.0 11.641111 7 GGACCTG 345 0.0 11.5996 6 TGTACTG 140 6.4392225E-10 11.570008 5 GGTATCA 475 0.0 11.446605 1 TGTAGGG 75 1.994572E-4 11.445696 2 TATAGTG 125 1.7444108E-8 11.433891 5 >>END_MODULE