FastQCFastQC Report
Thu 26 May 2016
SRR1926761_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926761_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740740
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12590.16996516996516997No Hit
GTCCTACAGTGTGCATTTCTCATTT10100.13635013635013635No Hit
CTGTAGGACGTGGAATATGGCAAGA9310.1256851256851257No Hit
GTCCTAAAGTGTGTATTTCTCATTT9160.12366012366012366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG902.3374014E-914.75172318
CCAACGA602.6077048E-514.22487719
TAGGACC2750.012.8129344
GTCCTAA3400.012.3449761
ACTAGGG550.002981549612.1359813
TTAAGGT550.003010492512.1203414
GTATAGG802.7431724E-511.9241261
AGGACCT4900.011.6514885
GTCCTAT1156.657501E-811.6130621
AATCCCG907.5762728E-611.5929899
CTAAATT2950.011.57278119
TGTAGGA7100.011.2813352
GGACCTG4050.011.2774916
GTCCTAC6350.011.266891
TAGGACA2200.011.2546034
TCTAAAT3550.011.21962218
TGGCGAG5450.011.13629418
ATTACTC600.00571030811.124653
AACCATA600.00580042611.101287
GTGTAGG1303.061359E-811.0068861
Produced by FastQC (version 0.11.5)