##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926761_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 740740 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.692219942219943 31.0 30.0 31.0 23.0 34.0 2 28.64930474930475 31.0 30.0 31.0 23.0 34.0 3 28.352092502092503 31.0 28.0 31.0 16.0 34.0 4 32.3036558036558 35.0 33.0 37.0 24.0 37.0 5 32.33222318222318 35.0 33.0 37.0 24.0 37.0 6 31.86854361854362 35.0 32.0 37.0 17.0 37.0 7 31.74946134946135 35.0 32.0 37.0 17.0 37.0 8 31.451412101412103 35.0 32.0 37.0 17.0 37.0 9 33.70814050814051 37.0 34.0 39.0 22.0 39.0 10 33.879145179145176 38.0 34.0 39.0 23.0 39.0 11 34.02734427734428 38.0 35.0 39.0 25.0 39.0 12 33.92201582201582 38.0 34.0 39.0 23.0 39.0 13 34.01697896697897 38.0 35.0 39.0 25.0 39.0 14 35.07830277830278 39.0 36.0 41.0 25.0 41.0 15 34.65846720846721 39.0 34.0 41.0 19.0 41.0 16 34.7546575046575 39.0 34.0 41.0 22.0 41.0 17 34.97360747360747 39.0 35.0 41.0 25.0 41.0 18 35.02568512568513 39.0 36.0 41.0 25.0 41.0 19 34.392946242946245 39.0 34.0 41.0 18.0 41.0 20 34.6992871992872 39.0 34.0 41.0 19.0 41.0 21 34.69799794799795 39.0 35.0 41.0 19.0 41.0 22 34.718512568512566 39.0 35.0 41.0 19.0 41.0 23 34.71918621918622 39.0 35.0 41.0 19.0 41.0 24 33.41837081837082 39.0 33.0 41.0 2.0 41.0 25 33.99832194832195 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.005244379527528764 1101 2 -0.4717462734814042 1101 3 -1.3812465938131169 1101 4 -0.4237894854863775 1101 5 -0.5789981479839952 1101 6 -2.30883097023759 1101 7 -0.9928466486848002 1101 8 -0.371370665532492 1101 9 -0.42454095386111845 1101 10 -9.514432743332879 1101 11 -4.097517414965555 1101 12 -1.7195639018240385 1101 13 -0.9364232729189368 1101 14 -0.8074156612641517 1101 15 -2.2197828895913148 1101 16 -0.5499733436244227 1101 17 -0.5435066146921415 1101 18 -0.45935512677522894 1101 19 -1.0334560061958058 1101 20 -0.30089802188685866 1101 21 -0.41583050221937157 1101 22 -0.1795663597423811 1101 23 -0.2817410224135912 1101 24 -0.5660940033416608 1101 25 -0.4476737936968007 1102 1 -0.14771358173880245 1102 2 -0.35071859471068123 1102 3 -0.5249485751723597 1102 4 -0.3631419569995593 1102 5 -0.25099055728244934 1102 6 -1.1982444497271558 1102 7 -0.2588784958738195 1102 8 0.3020627466471737 1102 9 0.0603860929032507 1102 10 0.1873143642107351 1102 11 -0.13106588653236884 1102 12 -0.0942640872535847 1102 13 -0.1284528785479182 1102 14 -0.3189172619840974 1102 15 0.1965817606440794 1102 16 0.22318740588637098 1102 17 -0.028323239779787457 1102 18 -0.4377069570539618 1102 19 0.34513023352807437 1102 20 -0.11262196454014628 1102 21 -0.17486437564615898 1102 22 -0.19281696094897427 1102 23 -0.24835976081438105 1102 24 -3.815633584386042 1102 25 -2.2712396724522215 1103 1 -0.14751884007775118 1103 2 -0.4062804322553042 1103 3 -0.18343844782073404 1103 4 0.008965383347614875 1103 5 -0.04339594660086732 1103 6 0.5389139867485824 1103 7 0.3444209618334746 1103 8 0.30832824549207416 1103 9 0.24449705795811383 1103 10 0.40733239351935424 1103 11 0.09610026108494907 1103 12 -0.03615097689944946 1103 13 0.10564701450121561 1103 14 0.13851048336290717 1103 15 0.463508187558638 1103 16 0.5452210113868858 1103 17 0.1539133367874541 1103 18 0.0029384545027042464 1103 19 -1.7387413877433673 1103 20 -0.4120341274132784 1103 21 -0.021066363581596192 1103 22 -0.10161190269138132 1103 23 0.029482407351324014 1103 24 1.4674399101745408 1103 25 0.5544312047176163 1104 1 -0.39389975839311475 1104 2 -0.30363694257640006 1104 3 -1.7757778711522576 1104 4 -0.4435541540416885 1104 5 -0.41679815436069845 1104 6 -1.7533563763441364 1104 7 -0.29405565601100747 1104 8 0.17257764895716576 1104 9 -0.13799291979086092 1104 10 -0.14782753667715554 1104 11 -0.2176887149808735 1104 12 -0.0619866290154647 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1107 11 -0.24234346089290426 1107 12 0.10172327141656012 1107 13 -0.2229371634805517 1107 14 0.08698161657299863 1107 15 -1.3498829431015693 1107 16 -0.5533531795567868 1107 17 -0.2130678153342629 1107 18 -0.20738431806906732 1107 19 0.83931687706189 1107 20 0.7039323841355625 1107 21 0.3097914592440958 1107 22 0.06900708118061516 1107 23 -0.04652532729095071 1107 24 -3.7074182526354313 1107 25 -1.5210346799852132 1108 1 0.37020168584877666 1108 2 0.07200159299899056 1108 3 0.6341027169190703 1108 4 0.3820606064711356 1108 5 0.6319138931119141 1108 6 -3.002413944788742 1108 7 -1.421265656145163 1108 8 0.47313692190233425 1108 9 0.269137512140162 1108 10 0.583326440764111 1108 11 0.20851990038631385 1108 12 0.4689798310044182 1108 13 0.21649312619621952 1108 14 0.16547604541619165 1108 15 -0.6739845885872242 1108 16 0.14333217939052645 1108 17 0.23252683588765422 1108 18 0.24519583031977987 1108 19 0.35569721773089213 1108 20 -0.9620845821043673 1108 21 -0.04665153319619719 1108 22 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-0.9858466996565411 2215 1 0.3943328255215448 2215 2 -0.17567756138706514 2215 3 0.2990251577135439 2215 4 0.24816667508072499 2215 5 0.47137316470222856 2215 6 1.0923004163873813 2215 7 0.5788950204342633 2215 8 -0.6211816094171851 2215 9 -0.023031814769780112 2215 10 -0.4503011674719062 2215 11 -0.17430549727097855 2215 12 -0.395111229171377 2215 13 -0.09073911283535807 2215 14 -0.1753465635025293 2215 15 -0.25294092179597527 2215 16 -0.013346104721527752 2215 17 -0.5072481341492718 2215 18 -1.2719344997637023 2215 19 -0.015251215431099752 2215 20 -3.91290306722075 2215 21 -2.1057446552731562 2215 22 -1.354247734531107 2215 23 -1.3420835611548014 2215 24 -1.3727876331399251 2215 25 -1.0011293653298168 2216 1 -1.2362319640582236 2216 2 -1.6385986028556125 2216 3 -0.7321816083386388 2216 4 -0.976350421649645 2216 5 -1.1533949835054784 2216 6 -2.6209914493229505 2216 7 -10.8268324466213 2216 8 -11.976237849938641 2216 9 -6.69354581182537 2216 10 -4.107246462925257 2216 11 -2.8485097153794037 2216 12 -2.6467685016239173 2216 13 -2.418090277154164 2216 14 -2.46300428510294 2216 15 -3.4829843394230835 2216 16 -2.664330487129348 2216 17 -2.823610424581588 2216 18 -2.709976353690834 2216 19 -2.040644705005043 2216 20 -2.958273239920878 2216 21 -5.493904063950584 2216 22 -4.093693452793406 2216 23 -4.142512885519963 2216 24 -4.721523919574274 2216 25 -4.005243088460656 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34747.0 3 0.0 4 305.0 5 1885.0 6 2343.0 7 1940.0 8 1410.0 9 1351.0 10 1250.0 11 1123.0 12 1198.0 13 1174.0 14 1175.0 15 1215.0 16 1334.0 17 1473.0 18 1637.0 19 2003.0 20 2601.0 21 3483.0 22 4361.0 23 5365.0 24 6171.0 25 7061.0 26 8150.0 27 9033.0 28 10276.0 29 12111.0 30 14729.0 31 17908.0 32 23024.0 33 31001.0 34 42429.0 35 59491.0 36 87890.0 37 141821.0 38 196272.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.792945735804736 18.23443128276325 12.024400079024215 25.948222902407803 2 14.468424503369452 20.011220234273082 41.805511433148354 23.71484382920911 3 19.04600967400914 23.981295473656033 29.02473117988844 27.94796367244638 4 11.583620301833673 16.34957153992994 39.198118369271384 32.868689788965014 5 12.60712988456236 37.72632812700436 37.08182717976486 12.584714808668423 6 32.29446149566758 37.23964627676453 18.256796371487717 12.209095856080175 7 27.966527355263032 31.54475903459499 22.359547975787706 18.129165634354276 8 24.637271158336436 37.61578288382796 20.60884572434097 17.138100233494637 9 26.087390950347217 15.556242683326966 19.94469371579645 38.41167265052937 10 13.765199682366477 29.69184686606993 35.44962969765394 21.093323753909647 11 34.68211682675819 22.627724413935386 24.271081718240712 18.419077041065705 12 22.695916468743967 24.95338295915929 32.3949614438928 19.955739128203952 13 28.860837430109516 22.11354494796948 25.67136046274555 23.35425715917545 14 20.16472907468168 22.236264700719676 27.984094192624998 29.614912031973645 15 22.897043574067272 31.353426299984875 23.05920413057098 22.690325995376874 16 21.759560568948707 28.601771010837247 28.159153328274332 21.479515091939714 17 21.380685738513076 28.967071426931838 28.149089323159643 21.503153511395446 18 21.168571123811994 28.194066951018794 29.70143026689404 20.935931658275173 19 23.316292359378206 27.431782091583756 27.340095710319645 21.91182983871839 20 22.90567061883023 27.85741965711909 28.343912493400687 20.892997230649993 21 22.525765470421526 27.245980748639997 27.24409044817674 22.984163332761742 22 22.46559583477359 28.46715131351538 27.60164621500173 21.465606636709296 23 21.866852100647442 28.194111570945374 27.814962091803324 22.124074236603857 24 22.800842005203798 27.37070507809072 28.3182984905747 21.510154426130786 25 22.176989788271705 27.740444141994736 28.219232830915765 21.863333238817802 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 199.0 1 199.0 2 273.0 3 347.0 4 347.0 5 347.0 6 546.5 7 746.0 8 746.0 9 746.0 10 1086.0 11 1426.0 12 1426.0 13 1426.0 14 2378.0 15 3330.0 16 3330.0 17 3330.0 18 6004.5 19 8679.0 20 8679.0 21 8679.0 22 13544.0 23 18409.0 24 18409.0 25 18409.0 26 26151.5 27 33894.0 28 33894.0 29 33894.0 30 44740.5 31 55587.0 32 55587.0 33 55587.0 34 64361.0 35 73135.0 36 73135.0 37 73135.0 38 79634.5 39 86134.0 40 86134.0 41 86134.0 42 90534.5 43 94935.0 44 94935.0 45 94935.0 46 97183.0 47 99431.0 48 99431.0 49 99431.0 50 95030.5 51 90630.0 52 90630.0 53 90630.0 54 79720.5 55 68811.0 56 68811.0 57 68811.0 58 58723.0 59 48635.0 60 48635.0 61 48635.0 62 39165.5 63 29696.0 64 29696.0 65 29696.0 66 22765.0 67 15834.0 68 15834.0 69 15834.0 70 11374.0 71 6914.0 72 6914.0 73 6914.0 74 4803.0 75 2692.0 76 2692.0 77 2692.0 78 1789.5 79 887.0 80 887.0 81 887.0 82 584.0 83 281.0 84 281.0 85 281.0 86 179.5 87 78.0 88 78.0 89 78.0 90 51.5 91 25.0 92 25.0 93 25.0 94 14.0 95 3.0 96 3.0 97 3.0 98 2.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23301023301023302 2 0.13567513567513567 3 0.13851013851013852 4 0.07168507168507168 5 0.022545022545022546 6 0.021735021735021735 7 0.28444528444528444 8 0.2652752652752653 9 0.018495018495018493 10 0.035640035640035636 11 0.01647001647001647 12 0.017685017685017686 13 0.015795015795015795 14 0.01755001755001755 15 0.01566001566001566 16 0.019710019710019712 17 0.018495018495018493 18 0.014715014715014716 19 0.023220023220023218 20 0.017955017955017954 21 0.015795015795015795 22 0.017820017820017818 23 0.018225018225018225 24 0.017145017145017146 25 0.016335016335016335 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 740740.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.53396922667159 #Duplication Level Percentage of deduplicated Percentage of total 1 93.48148773031514 81.82805670248884 2 5.057477226743686 8.854021118607482 3 0.7778914230631403 2.042757716643021 4 0.26574167038445945 0.9304569279071031 5 0.11638865453460544 0.5093980452182931 6 0.06790539674844533 0.35664173455820025 7 0.04550972568021409 0.2788552849044288 8 0.030870701500501446 0.21617880281205262 9 0.022177391641360008 0.17471476057163854 >10 0.11543803765655622 1.8408652424642207 >50 0.008785821968187993 0.5321240940179185 >100 0.00909323830675188 1.5279872811045734 >500 9.247360927626984E-4 0.6018321768054848 >1k 3.0824536425423275E-4 0.30611011189680454 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1259 0.16996516996516997 No Hit GTCCTACAGTGTGCATTTCTCATTT 1010 0.13635013635013635 No Hit CTGTAGGACGTGGAATATGGCAAGA 931 0.1256851256851257 No Hit GTCCTAAAGTGTGTATTTCTCATTT 916 0.12366012366012366 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.35000135000135E-4 0.0 11 0.0 0.0 0.0 2.7000027000027E-4 0.0 12 0.0 0.0 0.0 2.7000027000027E-4 1.35000135000135E-4 13 0.0 0.0 0.0 2.7000027000027E-4 1.35000135000135E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 90 2.3374014E-9 14.751723 18 CCAACGA 60 2.6077048E-5 14.224877 19 TAGGACC 275 0.0 12.812934 4 GTCCTAA 340 0.0 12.344976 1 ACTAGGG 55 0.0029815496 12.135981 3 TTAAGGT 55 0.0030104925 12.120341 4 GTATAGG 80 2.7431724E-5 11.924126 1 AGGACCT 490 0.0 11.651488 5 GTCCTAT 115 6.657501E-8 11.613062 1 AATCCCG 90 7.5762728E-6 11.592989 9 CTAAATT 295 0.0 11.572781 19 TGTAGGA 710 0.0 11.281335 2 GGACCTG 405 0.0 11.277491 6 GTCCTAC 635 0.0 11.26689 1 TAGGACA 220 0.0 11.254603 4 TCTAAAT 355 0.0 11.219622 18 TGGCGAG 545 0.0 11.136294 18 ATTACTC 60 0.005710308 11.12465 3 AACCATA 60 0.005800426 11.10128 7 GTGTAGG 130 3.061359E-8 11.006886 1 >>END_MODULE