FastQCFastQC Report
Thu 26 May 2016
SRR1926761_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926761_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740740
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12770.17239517239517238No Hit
GTCCTACAGTGGACATTTCTAAATT11840.15984015984015984No Hit
CTGTAGGACGTGGAATATGGCAAGA11490.1551151551151551No Hit
GTCCTAAAGTGTGTATTTCTCATTT9720.1312201312201312No Hit
GTCCTACAGTGTGCATTTCTCATTT9300.12555012555012557No Hit
GGTATCAACGCAGAGTACTTTTTTT8610.11623511623511623No Hit
CTTTAGGACGTGAAATATGGCGAGG8440.11394011394011394No Hit
CTGTAGGACCTGGAATATGGCGAGA8280.1117801117801118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGGT402.7895928E-416.60072110
GCGCCAC456.8351906E-414.755213
GTACCGT400.005178493414.2899696
GTGCGCC551.982394E-413.79800111
TAGGACC3700.013.3897074
CGTGCGC500.001514845113.28057710
GTCCTAG1403.6379788E-1212.9430611
GGCGTGC604.135877E-412.6490228
TTAGGAC4100.012.548143
TGGCGAG7050.012.37822218
TGCGCCA701.1050734E-412.19562412
GTCCTAC6150.012.0956971
GTAGCAC802.7787863E-511.9091153
GGCGAGG3600.011.85685719
ACGTGAA3450.011.8240868
TTTAGGA4600.011.8159732
AGGACCT5350.011.7532955
GACGTGA3500.011.6591067
ACTATAC907.211567E-611.6444683
AGGACGT5900.011.6265265
Produced by FastQC (version 0.11.5)