##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926761_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 740740 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.995121095121096 31.0 30.0 33.0 26.0 34.0 2 30.34236709236709 31.0 30.0 34.0 26.0 34.0 3 30.443919593919595 31.0 30.0 34.0 26.0 34.0 4 34.043964143964146 37.0 35.0 37.0 30.0 37.0 5 34.03500013500013 37.0 35.0 37.0 30.0 37.0 6 33.96988146988147 37.0 35.0 37.0 30.0 37.0 7 33.94939924939925 37.0 35.0 37.0 30.0 37.0 8 33.963801063801064 37.0 35.0 37.0 30.0 37.0 9 35.53748143748144 39.0 35.0 39.0 30.0 39.0 10 35.439150039150036 39.0 35.0 39.0 30.0 39.0 11 35.47223587223587 39.0 35.0 39.0 30.0 39.0 12 35.383100683100686 39.0 35.0 39.0 30.0 39.0 13 35.3998838998839 39.0 35.0 39.0 30.0 39.0 14 36.51673731673732 40.0 37.0 41.0 30.0 41.0 15 36.5054513054513 40.0 37.0 41.0 30.0 41.0 16 36.48425628425628 40.0 37.0 41.0 30.0 41.0 17 36.45604125604125 40.0 37.0 41.0 30.0 41.0 18 36.42068877068877 40.0 37.0 41.0 30.0 41.0 19 36.48344763344763 40.0 37.0 41.0 30.0 41.0 20 36.44702594702595 40.0 37.0 41.0 30.0 41.0 21 36.39511569511569 40.0 37.0 41.0 30.0 41.0 22 36.31412236412236 40.0 37.0 41.0 30.0 41.0 23 36.251938601938605 40.0 37.0 41.0 30.0 41.0 24 36.188708588708586 40.0 37.0 41.0 29.0 41.0 25 36.081201231201234 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2601871459298515 1101 2 0.2689636916420568 1101 3 0.5973404110467335 1101 4 0.42042774643465464 1101 5 0.5076412155828649 1101 6 0.45199233631096547 1101 7 0.3632899023954792 1101 8 0.434067793895089 1101 9 0.346135276263972 1101 10 0.473342506999856 1101 11 0.4740311016944645 1101 12 0.4936965258185353 1101 13 0.581191212732513 1101 14 0.6900749329780993 1101 15 0.5099476372179907 1101 16 0.6431360459658109 1101 17 0.7001312998020666 1101 18 0.6843592459248669 1101 19 0.7392322788653871 1101 20 0.6743415606574956 1101 21 0.733312567380267 1101 22 0.6627082800737085 1101 23 0.734417616880819 1101 24 0.8833605422023538 1101 25 0.8423397081061808 1102 1 -0.4544840301477677 1102 2 -0.4704245880434321 1102 3 -0.24043752622902304 1102 4 -0.3527834815569264 1102 5 -0.6419273117153423 1102 6 -0.5725969936424065 1102 7 -0.49899139367106926 1102 8 -0.5461209798485243 1102 9 -0.6109556488882717 1102 10 -0.5228156073764936 1102 11 -0.7062843414868851 1102 12 -0.6611548899845445 1102 13 -0.6113684169447282 1102 14 -0.6980627817821983 1102 15 -0.6289565222876945 1102 16 -0.8006463065995604 1102 17 -0.7078665422428756 1102 18 -0.6971705202099088 1102 19 -0.5865156110946756 1102 20 -0.6028941430874539 1102 21 -0.5483092431093155 1102 22 -0.4425472396298531 1102 23 -0.735022000997084 1102 24 -0.7157377859345857 1102 25 -0.6346448651968544 1103 1 -0.3440340983858725 1103 2 -0.31926893762983966 1103 3 -0.04740927769469039 1103 4 -0.17501889595618536 1103 5 -0.29088505120439834 1103 6 -0.16528146032509738 1103 7 -0.36632363707929727 1103 8 -0.537601817426129 1103 9 -0.3746075890020677 1103 10 -0.21310255293206382 1103 11 -0.11555278815514924 1103 12 -0.09294505270376874 1103 13 -0.19287958082455248 1103 14 -0.36806832563755165 1103 15 -0.3216298384397902 1103 16 -0.24709941704792726 1103 17 -0.3269251547713594 1103 18 -0.0982260514227562 1103 19 -0.12230881429660911 1103 20 -0.2406086926452815 1103 21 -0.46740664692028844 1103 22 -0.20839552017092444 1103 23 -0.5264725279774396 1103 24 -0.3716457610767563 1103 25 -0.32384659714123387 1104 1 -0.1325905380982988 1104 2 -0.03842138578433918 1104 3 -0.026270031504228797 1104 4 -0.08306038784316172 1104 5 -0.18512223751064738 1104 6 -0.1344668127413513 1104 7 -0.1334295949959099 1104 8 -0.3190021608691964 1104 9 -0.29358800593390555 1104 10 -0.06999146659592981 1104 11 -0.12840635412548806 1104 12 -0.17399715292472706 1104 13 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24 0.37790489420804363 1105 25 0.36223268059746516 1106 1 0.07797530990491097 1106 2 0.04396799624340275 1106 3 0.1092668713924354 1106 4 -0.03737862871872011 1106 5 0.11221665779339673 1106 6 0.2723337881482948 1106 7 0.11846279224887013 1106 8 0.06377822995508353 1106 9 -0.3703605765822573 1106 10 -0.10591722566501005 1106 11 -0.09245676243215684 1106 12 -0.18148213452747797 1106 13 -0.03393166618486987 1106 14 -0.10802452735673995 1106 15 0.006424844547758823 1106 16 -0.006049802676649563 1106 17 -0.11140394596352365 1106 18 -0.023324196018855048 1106 19 -0.1658182982614349 1106 20 -0.17367886564539958 1106 21 -0.13936740761426591 1106 22 -0.09610296742302893 1106 23 0.03686200996305189 1106 24 -0.03648235148251899 1106 25 0.02746781459349279 1107 1 0.6751325151582499 1107 2 0.5072009631372474 1107 3 0.5431540337521561 1107 4 0.695160233259088 1107 5 0.5079080046167732 1107 6 0.6696136757748619 1107 7 0.45010610304522203 1107 8 0.5086254195641615 1107 9 0.29748820156761724 1107 10 0.19087313819222373 1107 11 0.27499352407790667 1107 12 0.3912350539662981 1107 13 0.44551526841367206 1107 14 0.35344205450736865 1107 15 0.40232417868691783 1107 16 0.45425627025153403 1107 17 0.373538073804319 1107 18 0.4064062343340282 1107 19 0.45049031874349055 1107 20 0.1798031133086937 1107 21 0.3064081891435464 1107 22 0.3435264534377964 1107 23 0.09155513824299533 1107 24 0.10524593268240778 1107 25 0.0726281908998132 1108 1 0.394625573493947 1108 2 0.22316839824269508 1108 3 0.4976526004175561 1108 4 0.4510579448914527 1108 5 0.4382166756336048 1108 6 0.33527997911580343 1108 7 0.2906742938292197 1108 8 0.2028634436956125 1108 9 0.28131387535177055 1108 10 0.2938847480563709 1108 11 0.20326396699437055 1108 12 0.3318415254501019 1108 13 0.16692674502591842 1108 14 0.40471189253032946 1108 15 0.21700468095308167 1108 16 0.14214729591129327 1108 17 0.39564590849013825 1108 18 0.3087846054464194 1108 19 0.35155954836632475 1108 20 0.4685477109084033 1108 21 0.5796344923676031 1108 22 0.6021388803465015 1108 23 0.4012050890040797 1108 24 0.561852860027976 1108 25 0.4292143706905307 1109 1 0.3385085938268979 1109 2 0.2038784581000357 1109 3 0.3658925744581296 1109 4 0.353675327277287 1109 5 0.30734465908447106 1109 6 0.33159901351783816 1109 7 0.22431116316870003 1109 8 0.24455352986004897 1109 9 0.3255100892157259 1109 10 0.25995786324553194 1109 11 0.23046631724962197 1109 12 0.2884377115003858 1109 13 0.26869291317115085 1109 14 0.3478937765589052 1109 15 0.3981078986472113 1109 16 0.37902996277883716 1109 17 0.3000755254147549 1109 18 0.3440138074837691 1109 19 0.41969869784742286 1109 20 0.46294151773086867 1109 21 0.5914234329564394 1109 22 0.37384078732135606 1109 23 0.501734128215567 1109 24 0.44008112914843167 1109 25 0.4589648588784101 1110 1 0.16248331387275528 1110 2 0.022841933187113028 1110 3 0.2047862401392173 1110 4 0.355305773185961 1110 5 0.30094132039918264 1110 6 0.45875112900312587 1110 7 0.23775320264449817 1110 8 0.3370349076818897 1110 9 0.2665720286128561 1110 10 0.17800565651376132 1110 11 0.12367137908957915 1110 12 0.3040548974238604 1110 13 0.2311897327049195 1110 14 0.113295275691776 1110 15 0.004320116901766369 1110 16 0.11528230608787027 1110 17 0.1969750460782791 1110 18 -0.0829356355325146 1110 19 0.11240643712815768 1110 20 0.2962719264975462 1110 21 0.16717848295190407 1110 22 0.05626355682748141 1110 23 0.09015401409897095 1110 24 0.0938606928615755 1110 25 0.2589120704503358 1111 1 0.46030893512382676 1111 2 0.3976682376406657 1111 3 0.3586515450327994 1111 4 0.4562373603000651 1111 5 0.4013771514744704 1111 6 0.4993966106029788 1111 7 0.4239323927600651 1111 8 0.3921702165125751 1111 9 0.5728696501418398 1111 10 0.41699480633196373 1111 11 0.31569542434306186 1111 12 0.427170300744919 1111 13 0.35250955831458697 1111 14 0.26669739246150215 1111 15 0.40851399587071313 1111 16 0.2531046560728143 1111 17 0.20393010525056354 1111 18 0.3693516453216077 1111 19 0.1912055929677976 1111 20 0.5095700575334661 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0.0615084686308478 2214 8 0.11272234164837158 2214 9 0.2741062641143017 2214 10 -0.058828586790447446 2214 11 0.10193400603385783 2214 12 -0.1580901032193367 2214 13 0.12890816262029148 2214 14 0.08562850569121849 2214 15 3.828848581406419E-4 2214 16 -0.11115950041345002 2214 17 -0.22626439400669085 2214 18 -0.10409940006339724 2214 19 -0.11046156331892121 2214 20 -0.15499770957736558 2214 21 -0.12729498167873032 2214 22 -0.1469478073696422 2214 23 -0.20183311063908604 2214 24 -0.2989107150029042 2214 25 -0.17668807098353057 2215 1 0.35830852894998344 2215 2 0.21344918918522637 2215 3 0.09288760887893588 2215 4 0.2931942400067413 2215 5 0.2041584817486921 2215 6 0.10222377265536409 2215 7 -0.026789526356452598 2215 8 0.21374600635603258 2215 9 0.021864644323670746 2215 10 0.15137220769758386 2215 11 -0.02767310157521763 2215 12 -0.15758830026063464 2215 13 -0.08643194716326974 2215 14 -0.13938313973601169 2215 15 -0.17500824489157907 2215 16 -0.026848606491128635 2215 17 -0.06584205317918901 2215 18 0.06355076930342562 2215 19 -0.1536572684720312 2215 20 -0.07244412561799862 2215 21 -0.11279467546563637 2215 22 -0.08023800208651721 2215 23 -0.10549160740269059 2215 24 -0.2373655484049877 2215 25 0.13162686401865642 2216 1 -0.7468156382550113 2216 2 -0.5179645453129353 2216 3 -2.3644828521630323 2216 4 -1.991034570333639 2216 5 -1.0527236003011922 2216 6 -0.6598685632739745 2216 7 -0.860159826592394 2216 8 -0.6351393029044559 2216 9 -0.6779028800675491 2216 10 -0.8415779729319439 2216 11 -0.776359621316935 2216 12 -0.6586612646088312 2216 13 -0.935232001504616 2216 14 -0.9609799435776125 2216 15 -0.9286839102265105 2216 16 -0.8556116754464185 2216 17 -0.9908801059845587 2216 18 -0.8445527454172534 2216 19 -0.9156255342344863 2216 20 -0.9722120658868789 2216 21 -0.7669383417875864 2216 22 -0.8770124549994662 2216 23 -0.8472419809994491 2216 24 -1.0428540244351865 2216 25 -1.052127291292237 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15856.0 3 15.0 4 237.0 5 1815.0 6 2342.0 7 1710.0 8 1380.0 9 1210.0 10 1042.0 11 1015.0 12 948.0 13 868.0 14 855.0 15 879.0 16 917.0 17 938.0 18 1055.0 19 1237.0 20 1582.0 21 2060.0 22 2560.0 23 3158.0 24 3819.0 25 4620.0 26 5553.0 27 6210.0 28 7047.0 29 8839.0 30 11460.0 31 14498.0 32 19185.0 33 25513.0 34 34717.0 35 48819.0 36 72405.0 37 126501.0 38 307875.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.21481580594108 19.072062538265357 10.208150031288055 28.504971624505508 2 14.430396513878527 20.759669553746175 39.74275797190774 25.06717596046756 3 18.5353557174567 24.273065062297135 29.27641349960305 27.915165720643113 4 12.006777006777007 17.134217134217135 37.85187785187785 33.007128007128 5 12.762656946132036 38.54043472390982 35.75806669366815 12.938841636289997 6 32.02826098737332 38.16655828320598 17.4666720858397 12.338508643580989 7 28.460337736200643 31.619896891347025 21.542632916451744 18.377132456000584 8 24.976777844984014 38.55903827875227 19.553864598634753 16.910319277628965 9 26.018464263630154 15.649935059148829 19.583514474654166 38.74808620256685 10 13.915841002704138 30.116900246652907 34.56070267052059 21.406556080122368 11 34.702873320363814 22.827661530610456 24.069306382651263 18.40015876637447 12 22.901588818733725 25.113569016887187 31.98535516558464 19.999486998794445 13 29.061152526264344 22.125096862858392 25.45731419206251 23.35643641881475 14 20.339850257445953 22.48581819752733 27.547526925849624 29.62680461917709 15 22.610642049243847 31.537472898064323 23.018082357483642 22.83380269520819 16 21.768425543174512 28.84812173805043 27.684552432844 21.698900285931057 17 21.3024813024813 29.26141426141426 27.73078273078273 21.705321705321705 18 21.261064963502665 28.290396482431735 29.499324323412164 20.949214230653443 19 23.0990630990631 27.49331749331749 27.52855252855253 21.87906687906688 20 22.975091024209675 27.90639592729943 28.12118201196916 20.997331036521736 21 22.466944888119603 27.156295019151916 27.35490247172443 23.021857621004056 22 22.495882495882498 28.51391851391851 27.363987363987363 21.626211626211624 23 21.815241265816976 28.251381390746268 27.711515122060536 22.22186222137622 24 22.51869751869752 27.503847503847506 28.417258417258417 21.56019656019656 25 22.330976588710037 27.825022174640313 27.962187681835427 21.881813554814226 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 224.0 1 224.0 2 221.0 3 218.0 4 218.0 5 218.0 6 397.5 7 577.0 8 577.0 9 577.0 10 906.5 11 1236.0 12 1236.0 13 1236.0 14 2157.5 15 3079.0 16 3079.0 17 3079.0 18 5526.5 19 7974.0 20 7974.0 21 7974.0 22 12822.5 23 17671.0 24 17671.0 25 17671.0 26 25533.5 27 33396.0 28 33396.0 29 33396.0 30 43894.0 31 54392.0 32 54392.0 33 54392.0 34 62966.0 35 71540.0 36 71540.0 37 71540.0 38 78573.0 39 85606.0 40 85606.0 41 85606.0 42 90110.5 43 94615.0 44 94615.0 45 94615.0 46 97694.5 47 100774.0 48 100774.0 49 100774.0 50 96281.0 51 91788.0 52 91788.0 53 91788.0 54 81195.5 55 70603.0 56 70603.0 57 70603.0 58 59961.0 59 49319.0 60 49319.0 61 49319.0 62 39893.5 63 30468.0 64 30468.0 65 30468.0 66 23321.5 67 16175.0 68 16175.0 69 16175.0 70 11606.5 71 7038.0 72 7038.0 73 7038.0 74 4889.5 75 2741.0 76 2741.0 77 2741.0 78 1839.0 79 937.0 80 937.0 81 937.0 82 612.0 83 287.0 84 287.0 85 287.0 86 177.0 87 67.0 88 67.0 89 67.0 90 40.0 91 13.0 92 13.0 93 13.0 94 7.0 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11353511353511354 2 0.12042012042012042 3 0.01296001296001296 4 0.0 5 0.0054000054000054 6 0.3537003537003537 7 0.02187002187002187 8 0.00918000918000918 9 0.008910008910008909 10 0.003105003105003105 11 0.003915003915003915 12 1.35000135000135E-4 13 2.7000027000027E-4 14 2.7000027000027E-4 15 0.00243000243000243 16 2.7000027000027E-4 17 0.0 18 1.35000135000135E-4 19 0.0 20 4.05000405000405E-4 21 0.011205011205011205 22 0.0 23 1.35000135000135E-4 24 0.0 25 0.003915003915003915 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 740740.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.06954853433622 #Duplication Level Percentage of deduplicated Percentage of total 1 91.83939179526398 78.12735597691129 2 6.356203534564011 10.81438730155425 3 0.9964130464121973 2.5429322403602472 4 0.3068334717565734 1.0440873967021882 5 0.15039110485362225 0.6396851696738833 6 0.08552990801492764 0.436559439660792 7 0.05463247159956987 0.32532917860032484 8 0.03380256135177431 0.23004549067997143 9 0.02921662917043782 0.22369009079018376 >10 0.12455942382237008 1.9494007370194923 >50 0.010957723364950574 0.6218421232564899 >100 0.010004010273958013 1.563070614982694 >500 0.001587938116653533 0.993956744758198 >1k 4.763814349960599E-4 0.48765749504989053 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1277 0.17239517239517238 No Hit GTCCTACAGTGGACATTTCTAAATT 1184 0.15984015984015984 No Hit CTGTAGGACGTGGAATATGGCAAGA 1149 0.1551151551151551 No Hit GTCCTAAAGTGTGTATTTCTCATTT 972 0.1312201312201312 No Hit GTCCTACAGTGTGCATTTCTCATTT 930 0.12555012555012557 No Hit GGTATCAACGCAGAGTACTTTTTTT 861 0.11623511623511623 No Hit CTTTAGGACGTGAAATATGGCGAGG 844 0.11394011394011394 No Hit CTGTAGGACCTGGAATATGGCGAGA 828 0.1117801117801118 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGGT 40 2.7895928E-4 16.600721 10 GCGCCAC 45 6.8351906E-4 14.7552 13 GTACCGT 40 0.0051784934 14.289969 6 GTGCGCC 55 1.982394E-4 13.798001 11 TAGGACC 370 0.0 13.389707 4 CGTGCGC 50 0.0015148451 13.280577 10 GTCCTAG 140 3.6379788E-12 12.943061 1 GGCGTGC 60 4.135877E-4 12.649022 8 TTAGGAC 410 0.0 12.54814 3 TGGCGAG 705 0.0 12.378222 18 TGCGCCA 70 1.1050734E-4 12.195624 12 GTCCTAC 615 0.0 12.095697 1 GTAGCAC 80 2.7787863E-5 11.909115 3 GGCGAGG 360 0.0 11.856857 19 ACGTGAA 345 0.0 11.824086 8 TTTAGGA 460 0.0 11.815973 2 AGGACCT 535 0.0 11.753295 5 GACGTGA 350 0.0 11.659106 7 ACTATAC 90 7.211567E-6 11.644468 3 AGGACGT 590 0.0 11.626526 5 >>END_MODULE