FastQCFastQC Report
Thu 26 May 2016
SRR1926760_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926760_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792658
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12740.16072505418478084No Hit
GTCCTACAGTGTGCATTTCTCATTT11030.13915206810503394No Hit
CTGTAGGACGTGGAATATGGCAAGA9560.12060686954525154No Hit
GTATCAACGCAGAGTACTTTTTTTT9230.1164436617053004No Hit
GTCCTAAAGTGTGTATTTCTCATTT8940.11278508511867666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT402.7358686E-416.643338
TCCAACG851.0459189E-915.61588518
CCGTGTC456.850257E-414.7511279
TACCGTA400.00520691714.2783627
TAAGACC551.9141234E-413.855344
GGCGAGG2050.012.94975819
CCTCTAT751.4055895E-512.727351
CCAACGA751.5070007E-512.64143119
TAGGACC2800.012.2471294
TAACACT550.0030050912.1234234
GTCCTAC6650.012.0574881
GTAGGAC6500.011.8823453
AGGACGT4850.011.7759695
GCATAGG657.700645E-411.74832251
TAGGACG4950.011.7385514
CCTACAC1302.4574547E-911.7356513
ACTGTTC1552.5465852E-1111.6588
GTAAGAC752.0013635E-411.4422593
AGTATTG752.0334493E-411.4226895
TGTAGGA7200.011.3892852
Produced by FastQC (version 0.11.5)