##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926760_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 792658 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.759005775504694 31.0 30.0 33.0 23.0 34.0 2 28.7556436697794 31.0 30.0 31.0 23.0 34.0 3 28.46433266301482 31.0 28.0 33.0 16.0 34.0 4 32.40133702050569 35.0 33.0 37.0 25.0 37.0 5 32.448813990396864 35.0 33.0 37.0 25.0 37.0 6 31.96779317183451 35.0 32.0 37.0 18.0 37.0 7 31.837134047722977 35.0 32.0 37.0 17.0 37.0 8 31.54848623239783 35.0 32.0 37.0 17.0 37.0 9 33.774817134249574 37.0 34.0 39.0 23.0 39.0 10 33.93582856667062 38.0 34.0 39.0 24.0 39.0 11 34.08488149996594 38.0 35.0 39.0 25.0 39.0 12 33.96373972129216 38.0 35.0 39.0 24.0 39.0 13 34.069476369379984 38.0 35.0 39.0 25.0 39.0 14 35.13329834556644 39.0 36.0 41.0 25.0 41.0 15 34.70128731432724 39.0 34.0 41.0 19.0 41.0 16 34.796263962515994 39.0 34.0 41.0 22.0 41.0 17 35.04408837102508 39.0 35.0 41.0 25.0 41.0 18 35.08284026654623 39.0 36.0 41.0 25.0 41.0 19 34.47035039071075 39.0 34.0 41.0 18.0 41.0 20 34.74667637240777 39.0 34.0 41.0 19.0 41.0 21 34.72770854517333 39.0 35.0 41.0 19.0 41.0 22 34.74976219252187 39.0 35.0 41.0 19.0 41.0 23 34.76980740748217 39.0 35.0 41.0 19.0 41.0 24 33.47070741732248 39.0 33.0 41.0 2.0 41.0 25 34.05351488283724 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.13066095135454248 1101 2 -0.020822949711501337 1101 3 -1.1468027944790897 1101 4 -0.33097219125186683 1101 5 -0.12361252549327872 1101 6 -2.2511646833814467 1101 7 -0.5340120669173913 1101 8 -0.04994146245988418 1101 9 -0.14375580501413054 1101 10 -9.012650988174919 1101 11 -4.159393859486947 1101 12 -1.6049558722727113 1101 13 -0.6948963444217213 1101 14 -0.5237800047098062 1101 15 -1.8516973239969872 1101 16 -0.27418819910191417 1101 17 -0.21428906848932883 1101 18 -0.08401213419271159 1101 19 -0.7731937360627157 1101 20 -0.006511024146007571 1101 21 -0.06797540741334274 1101 22 0.11355589508777797 1101 23 0.11633329019988281 1101 24 -0.07498115597405075 1101 25 -0.051875641689107965 1102 1 -0.3904181128198907 1102 2 -0.4783656707734707 1102 3 -0.6104906286288241 1102 4 -0.3640010688624358 1102 5 -0.2815389845523839 1102 6 -1.0988012463622248 1102 7 -0.07966568884999248 1102 8 0.14613774907936872 1102 9 0.13397530723659656 1102 10 0.2011576851290613 1102 11 -0.1390530930719791 1102 12 -0.07662940614009273 1102 13 -0.21399335173691014 1102 14 -0.6115917549912737 1102 15 -0.0324962771172963 1102 16 -0.01570587993328587 1102 17 -0.28999185132663285 1102 18 -0.23180431442577998 1102 19 -0.11849751390730034 1102 20 -0.33267947982162127 1102 21 -0.37347299947214196 1102 22 -0.4089231348980178 1102 23 -0.6449592761290219 1102 24 -4.074567060823529 1102 25 -2.385714436362754 1103 1 -0.05200431878182954 1103 2 -0.29793478024731 1103 3 -0.02541549834226231 1103 4 0.04073125831682489 1103 5 -0.29417990560045837 1103 6 0.5316178238863642 1103 7 0.21174255041049506 1103 8 0.18598413064395203 1103 9 0.004787955084182727 1103 10 0.17957476174102283 1103 11 -0.08492224485789279 1103 12 -0.12407088070843031 1103 13 0.043311504514960575 1103 14 -0.09148147255439909 1103 15 0.33555328673705276 1103 16 0.3152415642516644 1103 17 -0.09899423495713222 1103 18 -0.2097802010595302 1103 19 -2.2934044950987555 1103 20 -0.6774897756598293 1103 21 -0.42325970745707764 1103 22 -0.2086989260660843 1103 23 -0.3569149222314181 1103 24 1.3897085970840877 1103 25 0.506062264289092 1104 1 -0.018656454788803956 1104 2 -0.001169452864157705 1104 3 -1.0682553310672773 1104 4 -0.13726883034482995 1104 5 0.0404934500953118 1104 6 -1.1608193371675313 1104 7 0.1589016832995327 1104 8 0.6669787666139868 1104 9 0.580039532522747 1104 10 0.7371841480546166 1104 11 0.5311365969253856 1104 12 0.6020407147580045 1104 13 0.49002585144801003 1104 14 0.41626164508483754 1104 15 0.7553802487455314 1104 16 0.2426047667105422 1104 17 0.4412382063414668 1104 18 0.4152098245045792 1104 19 -0.6988054674215434 1104 20 0.11985850208757398 1104 21 0.41399370232230126 1104 22 0.5153867506439767 1104 23 0.5704867002768168 1104 24 1.8330190754137021 1104 25 1.160272215081875 1105 1 -0.11545780159014996 1105 2 -0.26208367988008163 1105 3 0.17505916987434134 1105 4 -0.14651096924798424 1105 5 -0.15872186691661483 1105 6 -0.39829008989251946 1105 7 -0.012735593422490865 1105 8 0.19242532256901512 1105 9 -0.7336909930721873 1105 10 -0.2882310205808878 1105 11 -0.44504042755671946 1105 12 -0.34863268591539054 1105 13 -0.46222262198690345 1105 14 -0.5629228552861036 1105 15 -0.07248598420544283 1105 16 -0.6229833175216086 1105 17 -0.5778880848187242 1105 18 -0.4744466270162775 1105 19 -1.3787860643512886 1105 20 -0.5601877228739056 1105 21 -0.6122118560866454 1105 22 -0.4678238868039486 1105 23 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1110 7 0.6679412313221356 1110 8 1.218996522869027 1110 9 0.7115972403516366 1110 10 0.7700332198867414 1110 11 0.44263780757913906 1110 12 0.5338809084626064 1110 13 0.39519130746091946 1110 14 0.2681583359645785 1110 15 0.7990848492297928 1110 16 0.4707322889058645 1110 17 0.15677492950531047 1110 18 0.236154134431942 1110 19 -2.043357525532919 1110 20 -0.27146514601573557 1110 21 0.03195173299219789 1110 22 0.3425457498368729 1110 23 0.19344876645922682 1110 24 -0.6249712393562774 1110 25 -0.11014747821917581 1111 1 0.6725432295048037 1111 2 0.55930303653426 1111 3 1.0982811290079013 1111 4 0.97517883061861 1111 5 1.2205350537767714 1111 6 1.25551579319513 1111 7 1.3586113939465996 1111 8 1.468681039432468 1111 9 0.8527376182182991 1111 10 0.700502008207387 1111 11 0.6502415157915209 1111 12 0.9005016460918682 1111 13 0.8764116821170091 1111 14 0.7994904118232924 1111 15 -0.03634650469579981 1111 16 0.17751774679161514 1111 17 0.4250763131732995 1111 18 0.6815543190399111 1111 19 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-2.6591239378185776 2216 12 -2.283415760825857 2216 13 -2.0226410311314282 2216 14 -2.399306865236994 2216 15 -3.374899718147841 2216 16 -2.3980749986642422 2216 17 -2.513754176735773 2216 18 -2.119774641771045 2216 19 -1.5886983506966956 2216 20 -2.500675402215087 2216 21 -5.024771636480825 2216 22 -3.5970192373368803 2216 23 -3.6549736137563116 2216 24 -3.9851805719287725 2216 25 -3.576945748860691 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36359.0 3 3.0 4 287.0 5 1885.0 6 2515.0 7 2122.0 8 1566.0 9 1381.0 10 1272.0 11 1291.0 12 1316.0 13 1298.0 14 1277.0 15 1301.0 16 1393.0 17 1627.0 18 1742.0 19 2180.0 20 2821.0 21 3730.0 22 4756.0 23 5646.0 24 6598.0 25 7710.0 26 8707.0 27 9688.0 28 10894.0 29 12739.0 30 15579.0 31 19006.0 32 24679.0 33 32891.0 34 44642.0 35 62304.0 36 91101.0 37 148362.0 38 219990.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.683821158260145 18.340573249615876 12.012823187965932 25.96278240415805 2 14.525117079968114 20.19826846717781 41.49955846292036 23.77705598993371 3 18.909020159767135 24.19285760308871 29.208858320688996 27.689263916455154 4 11.52389934010274 16.486321681923712 39.22531823914616 32.76446073882738 5 12.508501824027702 37.83264372341406 37.02353506789471 12.635319384663529 6 32.0311731051209 37.53530066449794 18.199874318277605 12.233651912103554 7 27.542856564337363 31.81054272748211 22.64266749684255 18.003933211337973 8 24.46924687913242 37.565691652588754 20.824301324272852 17.140760144005974 9 25.913181510755784 15.589574311296852 20.108163491919285 38.38908068602808 10 13.691258227161148 29.688582624992897 35.4728054067357 21.147353741110248 11 34.476549783604405 22.78639294411568 24.350750129332642 18.38630714294727 12 22.63619317765995 24.98807592042538 32.34908153042092 20.02664937149375 13 28.789119525979164 22.187299300097536 25.739805864443266 23.28377530948004 14 20.216350036340145 22.29164984252604 27.986579383025116 29.505420738108697 15 22.775787689943332 31.453594081805434 23.191413924109728 22.579204304141502 16 21.717470608499024 28.675025962439953 28.00183219746344 21.605671231597583 17 21.28401909915003 29.045824836970784 28.14853324180816 21.521622822071027 18 21.179877356347944 28.275670628611806 29.575163398692812 20.96928861634744 19 23.16148913134005 27.453051376595976 27.5269981399739 21.85846135209007 20 22.913732971952616 27.943410606221196 28.31526429750314 20.82759212432304 21 22.50309132662074 27.409594468417996 27.294647588765237 22.792666616196026 22 22.275932816752658 28.574078350172044 27.69283764805639 21.45715118501891 23 21.823908179990838 28.083789796178454 27.891993383023745 22.20030864080696 24 22.787012665830524 27.42244418479646 28.383169763515646 21.407373385857372 25 22.196299712440997 27.798590090254915 28.202737299267035 21.802372898037053 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 275.0 1 275.0 2 352.5 3 430.0 4 430.0 5 430.0 6 637.5 7 845.0 8 845.0 9 845.0 10 1310.0 11 1775.0 12 1775.0 13 1775.0 14 2898.0 15 4021.0 16 4021.0 17 4021.0 18 6818.0 19 9615.0 20 9615.0 21 9615.0 22 15049.5 23 20484.0 24 20484.0 25 20484.0 26 28982.0 27 37480.0 28 37480.0 29 37480.0 30 48490.5 31 59501.0 32 59501.0 33 59501.0 34 68721.0 35 77941.0 36 77941.0 37 77941.0 38 85150.0 39 92359.0 40 92359.0 41 92359.0 42 96847.0 43 101335.0 44 101335.0 45 101335.0 46 103517.0 47 105699.0 48 105699.0 49 105699.0 50 100979.5 51 96260.0 52 96260.0 53 96260.0 54 84996.5 55 73733.0 56 73733.0 57 73733.0 58 62395.0 59 51057.0 60 51057.0 61 51057.0 62 41240.0 63 31423.0 64 31423.0 65 31423.0 66 23978.0 67 16533.0 68 16533.0 69 16533.0 70 12040.5 71 7548.0 72 7548.0 73 7548.0 74 5241.5 75 2935.0 76 2935.0 77 2935.0 78 1968.0 79 1001.0 80 1001.0 81 1001.0 82 645.5 83 290.0 84 290.0 85 290.0 86 188.5 87 87.0 88 87.0 89 87.0 90 54.0 91 21.0 92 21.0 93 21.0 94 14.5 95 8.0 96 8.0 97 8.0 98 5.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24007831877051639 2 0.13940438373169767 3 0.14268448687832583 4 0.071279164532497 5 0.022834564213065407 6 0.022708406399733556 7 0.310852852049686 8 0.3044188035697615 9 0.019428303253105374 10 0.03948739557287001 11 0.016148200106477197 12 0.018166725119786842 13 0.015012779786490517 14 0.018419040746450552 15 0.015012779786490517 16 0.021068354826419465 17 0.0204375657597602 18 0.014886621973158662 19 0.024726931413043204 20 0.017914409493123135 21 0.014886621973158662 22 0.017535936053127577 23 0.019049829813109813 24 0.016652831359804606 25 0.015769726666481635 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 792658.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.51575856107262 #Duplication Level Percentage of deduplicated Percentage of total 1 92.8440776370577 80.32475804673166 2 5.550323519924126 9.603808991711967 3 0.8561765811306746 2.2221829913623816 4 0.27251207803470995 0.9430635659290859 5 0.1321602873503541 0.5716973755882606 6 0.08371409909917636 0.43455532714932293 7 0.04800982056396225 0.2907524231130524 8 0.03753706713962616 0.25980382701941357 9 0.02329728803162795 0.18140242918248817 >10 0.13117814807906708 2.0715127619324614 >50 0.010069962230319019 0.5756125300264395 >100 0.009630290039985948 1.5619447129533588 >500 0.0010213943589598407 0.6588369253935581 >1k 2.9182695970281165E-4 0.30006809190658895 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1274 0.16072505418478084 No Hit GTCCTACAGTGTGCATTTCTCATTT 1103 0.13915206810503394 No Hit CTGTAGGACGTGGAATATGGCAAGA 956 0.12060686954525154 No Hit GTATCAACGCAGAGTACTTTTTTTT 923 0.1164436617053004 No Hit GTCCTAAAGTGTGTATTTCTCATTT 894 0.11278508511867666 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 1.2615781333185307E-4 0.0 12 0.0 1.2615781333185307E-4 0.0 1.2615781333185307E-4 1.2615781333185307E-4 13 0.0 1.2615781333185307E-4 0.0 1.2615781333185307E-4 1.2615781333185307E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTAT 40 2.7358686E-4 16.64333 8 TCCAACG 85 1.0459189E-9 15.615885 18 CCGTGTC 45 6.850257E-4 14.751127 9 TACCGTA 40 0.005206917 14.278362 7 TAAGACC 55 1.9141234E-4 13.85534 4 GGCGAGG 205 0.0 12.949758 19 CCTCTAT 75 1.4055895E-5 12.72735 1 CCAACGA 75 1.5070007E-5 12.641431 19 TAGGACC 280 0.0 12.247129 4 TAACACT 55 0.00300509 12.123423 4 GTCCTAC 665 0.0 12.057488 1 GTAGGAC 650 0.0 11.882345 3 AGGACGT 485 0.0 11.775969 5 GCATAGG 65 7.700645E-4 11.7483225 1 TAGGACG 495 0.0 11.738551 4 CCTACAC 130 2.4574547E-9 11.735651 3 ACTGTTC 155 2.5465852E-11 11.658 8 GTAAGAC 75 2.0013635E-4 11.442259 3 AGTATTG 75 2.0334493E-4 11.422689 5 TGTAGGA 720 0.0 11.389285 2 >>END_MODULE